A5074911655- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Insect Resistance and Genetics
- Research in Cotton Cultivation
- Plant-Microbe Interactions and Immunity
- Chromosomal and Genetic Variations
- Insect symbiosis and bacterial influences
- RNA regulation and disease
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogenic Bacteria Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Soil Carbon and Nitrogen Dynamics
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Insect-Plant Interactions and Control
- Plant and Biological Electrophysiology Studies
- Plant and Fungal Interactions Research
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Plant pathogens and resistance mechanisms
- RNA modifications and cancer
- Biochemical and biochemical processes
- Genetically Modified Organisms Research
- Signaling Pathways in Disease
Huazhong Agricultural University
2017-2024
Sana'a University
2018-2023
University of Oxford
2023
John Wiley & Sons (United Kingdom)
2023
Hudson Institute
2023
John Wiley & Sons (United States)
2023
Zero to Three
2020
Summary The base‐editing technique using CRISPR / nC as9 (Cas9 nickase) or dC (deactivated Cas9) fused with cytidine deaminase is a powerful tool to create point mutations. In this study, novel G. hirsutum ‐Base Editor 3 (Gh BE 3) system has been developed single‐base mutations in the allotetraploid genome of cotton ( Gossypium ). A sequence APOBEC ) and uracil glycosylase inhibitor UGI was inserted into our /Cas9 plasmid pRGEB 32‐GhU6.7). Three target sites were chosen for two genes, Gh CLA...
Summary The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for genome editing. A new effector with single nuclease domain, relatively small size, low‐frequency off‐target effects cleavage capability under high temperature has recently established designated CRISPR/Cas12b (C2c1). Cas12b also shown inducibility in mammalian systems. Therefore, this system is potentially valuable editing the genomes of plant species, such as cotton, that are resistant to temperatures. Using...
Abstract Insects pose significant challenges in cotton‐producing regions. Here, they describe a high‐throughput CRISPR/Cas9‐mediated large‐scale mutagenesis library targeting endogenous insect‐resistance‐related genes cotton. This targeted 502 previously identified using 968 sgRNAs, generated ≈2000 T0 plants and achieved 97.29% genome editing with efficient heredity, reaching upto 84.78%. Several potential resistance‐related mutants (10% of 200 lines) their that may contribute to...
Lignins and their antimicrobial-related polymers cooperatively enhance plant resistance to pathogens. Several isoforms of 4-coumarate-coenzyme A ligases (4CLs) have been identified as indispensable enzymes involved in lignin flavonoid biosynthetic pathways. However, roles plant-pathogen interaction are still poorly understood. This study uncovers the role Gh4CL3 cotton vascular pathogen Verticillium dahliae. The 4CL3-CRISPR/Cas9 mutant (CR4cl) exhibited high susceptibility V. was most...
The efficiency and accuracy of the CRISPR/Mb2Cas12a system were demonstrated in cotton, achieving an over 90% at target sites. Notably, Mb2Cas12a exhibited significant tolerance under different temperatures ranging from 22°C to 32°C. Additionally, revealed effective editing more relaxed VTTV PAM sites cotton genome, which expanded genome range by approximately 2.6-fold than wide-type LbCas12a. Finally, a multiplex was also developed based on Mb2Cas12a, enabling simultaneous eight using...
Cotton (Gossypium hirsutum) is an allotetraploid species and a typical thermophilic crop that can survive grow well under temperatures up to 45 °C. CRISPR/LbCpf1 (LbCas12a) temperature-sensitive system for plant genome editing (Malzahn et al., 2019) has been successfully applied in such as rice, soya bean, tobacco, maize cotton (Lee 2019; Li 2018; Tang 2017; Xu 2019). However, the temperature sensitivity of LbCpf1 not tested yet, high temperature-resistant crop. In order improve efficiency...
Abstract Background Upland cotton ( Gossypium hirsutum ), harboring a complex allotetraploid genome, consists of A and D sub-genomes. Every gene has multiple copies with high sequence similarity that makes genetic, genomic functional analyses extremely challenging. The recent accessibility CRISPR/Cas9 tool provides the ability to modify targeted locus efficiently in various complicated plant genomes. However, current transformation method targeting one requires complicated, long laborious...
Abstract Key message The transcriptomic, phenotypic and metabolomic analysis of transgenic plants overexpressing GhMPK31 in upland cotton revealed the regulation H 2 O burst synthesis defensive metabolites by . Mitogen-activated protein kinases (MAPKs) are a crucial class kinases, which play an essential role various biological processes plants. Upland ( G. hirsutum ) is most widely cultivated species with high economic value. To gain better understanding MAPK gene family, we conducted...
Abstract N 6 ‐methyladenosine (m A) is the most prevalent internal modification of mRNA and plays an important role in regulating plant growth. However, there still a lack effective tools to precisely modify m A sites individual transcripts plants. Here, programmable editing are developed by combining CRISPR/dCas13(Rx) with methyltransferase GhMTA ( T argeted RNA M ethylation E ditor, TME ) or demethyltransferase GhALKBH10 D emethylation TDE ). These editors enable efficient deposition...
Abstract Verticillium wilt is a disastrous disease in cotton‐growing regions China. As common management method, cotton rotation with cereal crops used to minimize the loss caused by dahliae . However, correlation between soil microbiome and control of under crop system unclear. Therefore, three cropping systems (fallow, continuous cropping, cotton–maize rotation) were designed applied for generations greenhouse conditions investigate different responses microbial community. The this study...
Sap-sucking insects cause severe damage to cotton production. Long non-coding RNAs (lncRNAs) play vital regulatory roles in various development processes and stress response, however, the function of lncRNAs during sap-sucking insect infection is largely unknown. In this study, transcriptome profiles between resistant (HR) susceptible (ZS) cultivars under whitefly infestation at different time points (0, 4, 12, 24, 48 h) were compared. A total 6,651 transcript 606 differentially expressed...
Genes encoding reporter proteins are used as visual marker-assisted tools in genetic transformation well plant breeding. In this study, the red fluorescent protein identified Discosoma sp. coral (DsRed2) was successfully a marker for cotton engineering. DsRed2 expressed two cultivars, JIN668 and YZ1, driven by CaMV-35S promoter via Agrobacterium-mediated transformation. Our results suggest that expression provides an early-stage selection tool transgenic calli observation. Red fluorescence...
Abstract The cotton bollworm causes severe mechanical damage to plants during feeding and leaves oral secretions (OSs) at the wounds. role these OSs play in invasion of is still largely unknown. Here, a novel H. armigera effector peptidyl prolyl trans‐isomerase 5 (PPI5) was isolated characterized. PPI5 induces programmed cell death (PCD) due unfolded protein response (UPR) tobacco leaf. We reveal that important for growth development on plants, as it renders more susceptible feeding....
Abstract Plant bugs (Miridae species) have become major agricultural pests that cause increasing and severe economic damage. Plant‐mediated RNA interference (RNAi) is emerging as an eco‐friendly, efficient, reliable strategy for pest management. In this study, we isolated characterized a lethal gene of Apolygus lucorum named it LIM ( AlLIM ), which produced A. mortality rates ranging from 38% to 81%. Downregulation the expression in by injection double‐stranded (dsRNA) led muscle structural...
SUMMARY The glutamate receptor (GLR) serves as a ligand‐gated ion channel that plays vital role in plant growth, development, and stress response. Nevertheless, research on GLRs cotton is still very limited. present study conducted comprehensive analysis of gene family cotton. In total, 41 members the GLR were identified unveiling distinct subgroups comparison to Arabidopsis . Among these members, third subgroup highlights its pivotal cotton's defense against insect infestation. Furthermore,...
Abstract Mitogen activated protein kinases (MAPKs) are a crucial class of kinases, which play essential role in various biological processes plants. Upland cotton (G. hirsutum) is the most widely cultivated species with high economic value. To gain better understanding MAPK gene family, we conducted comprehensive analysis family cotton. In this study, total 55 GhMPK genes were identified from whole genome G. hirsutum. Through an investigation expression patterns under diverse stress...
Abstract Background : Upland cotton ( Gossypium hirsutum ), harboring a complex allotetraploid genome consists of A and D subgenomes. The genes in have multiple copies with high sequence similarity that makes genetic, genomic functional analysis extremely challenging. recent accessibility CRISPR/Cas9 tool offers the ability to modify targeted locus efficiently various complicated plant genomes. However, current transformation method targeting one gene requires complicated, long laborious...
Abstract Background Ralstonia solanacearum causing bacterial wilt disease provokes tremendous losses in global crop production including tobacco. Transcriptional changes response to R. infection have already been intensively studied various plant species. However, the genetic networks regulating defense responses of different tobacco varieties having resistance backgrounds are quite limited. Results In this study, we performed a comparative proteome analysis two cultivars, resistant (R) and...
Abstract Background : Upland cotton ( Gossypium hirsutum ), harboring a complex allotetraploid genome, consists of A and D sub-genomes. Every gene has multiple copies with high sequence similarity that makes genetic, genomic functional analysis extremely challenging. The recent accessibility CRISPR/Cas9 tool provides the ability to modify targeted locus efficiently in various complicated plant genomes. However, current transformation method targeting one requires complicated, long laborious...