A5114860151- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Research in Cotton Cultivation
- Insect Resistance and Genetics
- Pluripotent Stem Cells Research
- RNA and protein synthesis mechanisms
- Plant tissue culture and regeneration
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced biosensing and bioanalysis techniques
- Cancer Genomics and Diagnostics
- Plant Molecular Biology Research
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- Cancer-related molecular mechanisms research
- Plant Gene Expression Analysis
- RNA Interference and Gene Delivery
- Connexins and lens biology
- Polysaccharides and Plant Cell Walls
- Genomics and Phylogenetic Studies
- RNA regulation and disease
- Circular RNAs in diseases
- Acute Myeloid Leukemia Research
- RNA modifications and cancer
- Plant Reproductive Biology
- Redox biology and oxidative stress
Xinjiang Academy of Agricultural Sciences
2014-2024
Nanfang Hospital
2021-2024
Southern Medical University
2021-2024
Shandong Academy of Agricultural Sciences
2024
Huazhong Agricultural University
2011-2024
National Health and Family Planning Commission
2024
China Pharmaceutical University
2024
University of Auckland
2017-2024
Tongji Hospital
2024
Qingdao University
2024
Summary The CRISPR /Cas9 system has been extensively applied for crop improvement. However, our understanding of Cas9 specificity is very limited in Cas9‐edited plants. To identify on‐ and off‐target mutation an edited crop, we described whole genome sequencing ( WGS ) 14 cotton plants targeted to three genes, negative (Ne) control wild‐type WT In total, 4188–6404 unique single‐nucleotide polymorphisms SNP s) 312–745 insertions/deletions (indels) were detected compared , reference sequences....
Summary The base‐editing technique using CRISPR / nC as9 (Cas9 nickase) or dC (deactivated Cas9) fused with cytidine deaminase is a powerful tool to create point mutations. In this study, novel G. hirsutum ‐Base Editor 3 (Gh BE 3) system has been developed single‐base mutations in the allotetraploid genome of cotton ( Gossypium ). A sequence APOBEC ) and uracil glycosylase inhibitor UGI was inserted into our /Cas9 plasmid pRGEB 32‐GhU6.7). Three target sites were chosen for two genes, Gh CLA...
Autosomal dominant osteopetrosis type II (ADO2) is a rare human genetic disease that has been broadly studied as an important model; however, there are no disease-specific induced pluripotent stem cells (ADO2-iPSCs) may be valuable for understanding the pathogenesis and potential source of autologous cell-based therapies. To generate first ADO2-iPSCs from Chinese family with ADO2 to identify their characteristics, blood samples were collected proband his parents used genotyping by...
Summary The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for genome editing. A new effector with single nuclease domain, relatively small size, low‐frequency off‐target effects cleavage capability under high temperature has recently established designated CRISPR/Cas12b (C2c1). Cas12b also shown inducibility in mammalian systems. Therefore, this system is potentially valuable editing the genomes of plant species, such as cotton, that are resistant to temperatures. Using...
Abstract Background Base editors (BEs) display diverse applications in a variety of plant species such as Arabidopsis, rice, wheat, maize, soybean, and cotton, where they have been used to mediate precise base pair conversions without the collateral generation undesirable double-stranded breaks (DSB). Studies single-nucleotide polymorphisms (SNPs) underpinning traits are still challenging, particularly polyploidy SNPs present multiple copies, simultaneous modification all alleles would be...
Abstract Background Somatic embryogenesis is a major process for plant regeneration. However, cell communication and the gene regulatory network responsible reprogramming during somatic are still largely unclear. Recent advances in single-cell technologies enable us to explore mechanism of regeneration at resolution. Results We generate high-resolution transcriptomic landscape hypocotyl tissue from highly regenerable cotton genotype Jin668 recalcitrant TM-1. identify nine putative clusters...
The efficiency and accuracy of the CRISPR/Mb2Cas12a system were demonstrated in cotton, achieving an over 90% at target sites. Notably, Mb2Cas12a exhibited significant tolerance under different temperatures ranging from 22°C to 32°C. Additionally, revealed effective editing more relaxed VTTV PAM sites cotton genome, which expanded genome range by approximately 2.6-fold than wide-type LbCas12a. Finally, a multiplex was also developed based on Mb2Cas12a, enabling simultaneous eight using...
Cotton (Gossypium hirsutum) is an allotetraploid species and a typical thermophilic crop that can survive grow well under temperatures up to 45 °C. CRISPR/LbCpf1 (LbCas12a) temperature-sensitive system for plant genome editing (Malzahn et al., 2019) has been successfully applied in such as rice, soya bean, tobacco, maize cotton (Lee 2019; Li 2018; Tang 2017; Xu 2019). However, the temperature sensitivity of LbCpf1 not tested yet, high temperature-resistant crop. In order improve efficiency...
Abstract Key message The transcriptomic, phenotypic and metabolomic analysis of transgenic plants overexpressing GhMPK31 in upland cotton revealed the regulation H 2 O burst synthesis defensive metabolites by . Mitogen-activated protein kinases (MAPKs) are a crucial class kinases, which play an essential role various biological processes plants. Upland ( G. hirsutum ) is most widely cultivated species with high economic value. To gain better understanding MAPK gene family, we conducted...
Calcium-dependent protein kinases (CDPKs) act as key signal transduction enzymes in plants, especially response to diverse stresses, including herbivory. In this study, a comprehensive analysis of the CDPK gene family upland cotton revealed that GhCPKs are widely expressed multiple tissues and respond positively various biotic abiotic stresses. We developed strategy for screening insect-resistance genes from CRISPR-Cas9 mutant library GhCPKs. The was created using 246 single-guide RNAs...
Functional divergence after gene duplication plays a central role in plant evolution. Among cereals, only Hordeum vulgare (barley), Triticum aestivum (wheat) and Secale cereale (rye) accumulate delphinidin-derived (blue) anthocyanins the aleurone layer of grains, whereas Oryza sativa (rice), Zea mays (maize) Sorghum bicolor (sorghum) do not. The underlying genetic basis for this natural occurrence remains elusive. Here, we mapped barley Blx1 locus involved blue to an approximately 1.13 Mb...
Upland cotton (Gossypium hirsutum), an allotetraploid, contains At- and Dt- subgenome most genes have multiple homologous copies, which pose a huge challenge to investigate genes' function due the functional redundancy. Therefore, it is of great significance establish effective techniques for genomics in cotton. In this study, we tested two novel genome editing vectors compared them with CRISPR/Cas9 system (pRGEB32-GhU6.7) developed our laboratory previously. first new vector, sgRNA...
20-30% of patients with nasopharyngeal carcinoma (NPC) develop distant metastasis or recurrence leading to poor survival, which the underlying key molecular events have yet be addressed. Here alternative splicing in 85 NPC samples are profiled using transcriptome analysis and it is revealed that long isoform GOLIM4 (-L) exon-7 highly expressed associated prognosis. Lines evidence demonstrate pro-tumorigenic function GOLIM4-L cells. It further RBFOX2 binds a GGAA motif promotes its inclusion...
T-2 toxin causes kidney fibrosis. Wnt/β-catenin signaling promotes fibrosis when sustained and activated. However, whether T-2-induced involves activation has not been explored yet. renal mitochondrial damage, leading to reactive oxygen species (mtROS) overproduction NLRP3-inflammasome activation. The activated can mediate the be mediated by mtROS further regulate through is unclear. In this study, first, we confirmed that caused in mice kidneys HK-2 cells. Second, T-2-exposed Third,...
TiO2 thin films are highly stable and can be deposited onto a wide variety of substrate materials under moderate conditions. We demonstrate that organic alkenes will graft to the surface when illuminated with UV light at 254 nm resulting layers provide starting point for preparation DNA-modified exhibiting excellent stability biomolecular selectivity. By using protected amino group distal end, grafted deprotected yield molecular exposed primary groups then used covalently link DNA...