A5082362753- Genetic Mapping and Diversity in Plants and Animals
- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Genetics and Plant Breeding
- Research in Cotton Cultivation
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Genetic and phenotypic traits in livestock
- Transgenic Plants and Applications
- Insect-Plant Interactions and Control
- Plant Pathogenic Bacteria Studies
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Insect and Pesticide Research
- Insect Resistance and Genetics
- Plant and Fungal Interactions Research
- Viral Infections and Immunology Research
- Wheat and Barley Genetics and Pathology
- Biochemical and biochemical processes
- Bioinformatics and Genomic Networks
- Plant and animal studies
- Plant biochemistry and biosynthesis
Tobacco Research Institute
2022-2025
Chinese Academy of Agricultural Sciences
2022-2025
Huazhong Agricultural University
2020-2023
Chengdu Women's and Children's Central Hospital
2020
Chongqing Normal University
2016
Abstract Background Millennia of directional human selection has reshaped the genomic architecture cultivated cotton relative to wild counterparts, but we have limited understanding selective retention and fractionation components. Results We construct a comprehensive variome based on 1961 cottons identify 456 Mb 357 sequence with domestication improvement signals 162 loci, 84 which are novel, including 47 loci associated 16 agronomic traits. Using pan-genome analyses, 32,569 8851...
Summary Despite the established significance of WRKY proteins and phenylpropanoid metabolism in plant immunity, how modulate aspects pathway remains undetermined. To understand better role defence, we identified a cotton ( Gossypium hirsutum ) protein, GhWRKY41, that is, universally rapidly induced three disease‐resistant cultivars following inoculation with pathogenic fungus, Verticillium dahliae . We show overexpression GhWRKY41 transgenic Arabidopsis enhances resistance to V. , while...
Abstract Background Base editors (BEs) display diverse applications in a variety of plant species such as Arabidopsis, rice, wheat, maize, soybean, and cotton, where they have been used to mediate precise base pair conversions without the collateral generation undesirable double-stranded breaks (DSB). Studies single-nucleotide polymorphisms (SNPs) underpinning traits are still challenging, particularly polyploidy SNPs present multiple copies, simultaneous modification all alleles would be...
Nicotiana tabacum is an allotetraploid hybrid of sylvestris and tomentosiformis a model organism in genetics. However, features subgenome evolution, expression coordination, genetic diversity complex traits regulation N. remain unresolved. Here we present chromosome-scale assemblies for all three species, genotype phenotypic data 5,196 germplasms. Chromosome rearrangements epigenetic modifications are associated with genome evolution coordination following polyploidization. Two subgenomes...
Cotton (Gossypium hirsutum) is an allotetraploid species and a typical thermophilic crop that can survive grow well under temperatures up to 45 °C. CRISPR/LbCpf1 (LbCas12a) temperature-sensitive system for plant genome editing (Malzahn et al., 2019) has been successfully applied in such as rice, soya bean, tobacco, maize cotton (Lee 2019; Li 2018; Tang 2017; Xu 2019). However, the temperature sensitivity of LbCpf1 not tested yet, high temperature-resistant crop. In order improve efficiency...
Abstract Salicylic acid (SA) and brassinosteroids (BRs) are well known to regulate diverse processes of plant development stress responses, but the mechanisms by which these phytohormones mediate growth defense trade-off largely unclear. In addition, little is about roles DEHYDRATION RESPONSIVE ELEMENT BINDING transcription factors, especially in biotic growth. Here, we identified a cotton (Gossypium hirsutum) APETALA2/ETHYLENE FACTOR gene GhTINY2 that strongly induced Verticillium dahliae....
Sap-sucking insects cause severe damage to cotton production. Long non-coding RNAs (lncRNAs) play vital regulatory roles in various development processes and stress response, however, the function of lncRNAs during sap-sucking insect infection is largely unknown. In this study, transcriptome profiles between resistant (HR) susceptible (ZS) cultivars under whitefly infestation at different time points (0, 4, 12, 24, 48 h) were compared. A total 6,651 transcript 606 differentially expressed...
Callose plays an important role in plant development and response to a wide range of biotic abiotic stresses. However, the systematic identification callose synthase (CalS), major enzyme for biosynthesis, has been delayed crops, especially Solanaceae. In current research, 18 CalS genes (NtCalS1–NtCalS18) were identified Nicotiana tabacum classified into four subfamilies. A comprehensive analysis their physicochemical properties, gene structure, evolutionary history highlighted conservation....
Multiparent Advanced Generation Inter-Cross (MAGIC) population is an ideal genetic and breeding material for quantitative trait locus (QTL) mapping molecular breeding. In this study, a MAGIC derived from eight tobacco parents was developed. Eight 560 homozygous lines were genotyped by 430K single-nucleotide polymorphism (SNP) chip assay phenotyped nicotine content under different conditions. Four QTLs associated with detected genome-wide association (GWAS), one major QTL, named qNIC7-1,...
Cotton (Gossypium hirsutum) is an important fiber crop worldwide. Insect attack causes cotton yield and quality losses. However, little known about the mechanism of response to insect attack. We simulated feeding by applying oral secretions (OS) wounds, combined transcriptome metabolome analysis investigate how OS from two major pest species (Helicoverpa armigera Spodoptera litura) affect defense responses. found that respectively 12,668 13,379 genes were differentially expressed in...
Agronomic traits such as plant height (PH), leaf number (LN), length (LL), and width (LW), which are closely related to yield quality, important in tobacco (Nicotiana tabacum L.). To identify quantitative trait loci (QTLs) associated with agronomic tobacco, 209 recombinant inbred lines (RILs) 537 multiparent advanced generation intercross (MAGIC) were developed. The biparental RIL MAGIC genotyped using a 430 K single-nucleotide polymorphism (SNP) chip assay, their repeatedly evaluated under...
Abstract Nicotiana tabacum is a model organism in plant molecular and pathogenic research has significant potential the production of biofuels active pharmaceutical compounds synthetic biology. Because large allotetraploid genome tobacco, its genomic features, genetic diversity regulation many complex traits remain unknown. In this study, we present nearly complete chromosome-scale assembly N. provide evidence that homoeologous exchange between subgenomes epigenetic remodelling are likely...
Abstract Despite the importance in understanding impact of climate change, genetics rapid response to changing environments and its role adaptive evolution remains elusive. Here, we studied flowering time environment changes using 514 Arabidopsis thaliana worldwide accessions with re-sequencing genomes measurements from ten unique variable temperature, drought, daylight competition stresses. We revealed a polygenic basis mean plasticity, underpinned by 52 plasticity QTL. Widespread...
This study reports the assembly of a near-complete genome Catharanthus roseus, consisting 561.7 Mb scaffolded into 8 pseudochromosomes with contig N50 24.7 and scaffold 71.1 Mb. The enables construction gene regulatory network vinblastine biosynthetic pathway provides insights high susceptibility C. roseus to Huanglongbing pathogen.
Abstract Phenotypic plasticity is the property of a given genotype to produce multiple phenotypes in response changing environmental conditions. Understanding genetic basis phenotypic and establishing predictive model highly relevant for future agriculture under climate. Here, we investigated leaf width tobacco MAGIC population across four different environments. Environmental changes not only resulted differences mean but also plasticity. A total 45 QTL were identified, including 14 43...
Abstract Photosynthesis is the most important reaction underlying carbon fixation. Despite its potential in boosting assimilation, nature variations genes photosynthesis pathway and their role adaptive traits variation remains elusive. In this study, we investigated genetic, transcriptomic of 1103 associated pathways, including 82 core genes, 24 plastid-encoded RNA polymerase related 2 nucleus-encoded polymerase-related 34 photomorphogenesis-related 40 involved transcription translation...
ABSTRACT Understanding the genetic, and transcriptomic changes that drive phenotypic plasticity of fitness traits is a central question in evolutionary biology. In this study, we utilised 152 natural Swedish Arabidopsis thaliana accessions with re‐sequenced genomes, transcriptomes methylomes measured flowering times (FTs) under two temperature conditions (10°C 16°C) to address question. We revealed northern exhibited advanced response decreased temperature, whereas southern delayed their...
Genomic prediction is a powerful approach for improving genetic gain and shortening the breeding cycles in animal crop programs. A series of statistical machine learning models has been developed to increase performance continuously. However, application these requires advanced R programming skills command-line tools perform quality control, format input files, install packages dependencies, posing challenges breeders. Here, we present ShinyGS, stand-alone Shiny with user-friendly interface...
To analyze the epidemiological characteristics of hand, foot and mouth disease (HFMD) in Sichuan Province from 2013 to 2019. study correlation between meteorological factors incidence HFMD construct a prediction model.The HMFD surveillance data 2019 were collected through Chinese Center for Disease Control Prevention China Meteorological Network. Spearman was used relationship factors. Multiple regression model support vector (SVR) models respectively.A total 615 840 cases 81 deaths reported...