A5068826381- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Allergic Rhinitis and Sensitization
- Renal Diseases and Glomerulopathies
- Asthma and respiratory diseases
- Systemic Lupus Erythematosus Research
- Immune Response and Inflammation
- Food Allergy and Anaphylaxis Research
- Contact Dermatitis and Allergies
- Immune cells in cancer
- Carbohydrate Chemistry and Synthesis
- Dermatology and Skin Diseases
- Reproductive System and Pregnancy
- Neuroinflammation and Neurodegeneration Mechanisms
- Phagocytosis and Immune Regulation
- Adenosine and Purinergic Signaling
- Acute Kidney Injury Research
- Endometriosis Research and Treatment
- Genomics, phytochemicals, and oxidative stress
- IL-33, ST2, and ILC Pathways
- Viral gastroenteritis research and epidemiology
- Toxoplasma gondii Research Studies
- Insects and Parasite Interactions
- Cell Adhesion Molecules Research
University of Virginia
2016-2025
Center for Neuroscience and Regenerative Medicine
2021
Immunité et Cancer
2008-2020
Virginia Commonwealth University Medical Center
1991-2019
Center for Rheumatology
2001-2018
Carter Center
2015-2016
Pediatric Nephrology of Alabama
2016
Duke University
2013
Durham VA Medical Center
2013
University of Southern Denmark
2013
Although CD103-expressing dendritic cells (DCs) are widely present in nonlymphoid tissues, the transcription factors controlling their development and relationship to other DC subsets remain unclear. Mice lacking factor Batf3 have a defect of CD8α+ conventional DCs (cDCs) within lymphoid tissues. We demonstrate that Batf3−/− mice also lack CD103+CD11b− lung, intestine, mesenteric lymph nodes (MLNs), dermis, skin-draining nodes. Notably, displayed reduced priming CD8 T after pulmonary Sendai...
Dendritic cells (DC) mediate airway Ag presentation and play key roles in asthma infections. Although DC subsets are known to perform different functions, their occurrence mouse lungs has not been clearly defined. In this study, three major lung populations have found. Two of them the myeloid plasmacytoid (PDC) well-characterized other lymphoid organs. The third largest population is integrin alpha(E) (CD103) beta(7)-positive I-A(high)CD11c(high)-DC population. This was found reside mucosa...
Abstract Previous work has shown that ischemia-reperfusion (IR) injury (IRI) is dependent on CD4+ T cells from naive mice acting within 24 h. We hypothesize NKT are key participants in the early innate response IRI. Kidneys C57BL/6 were subjected to IRI (0.5, 1, 3, and h of reperfusion). After 30 min reperfusion, we observed a significant increase (145% control) single-cell kidney suspensions as measured by flow cytometry. A fraction expressed activation marker, CD69+, adhesion molecule,...
The nervous and immune systems interact in complex ways to maintain homeostasis respond stress or injury, rapid nerve conduction can provide instantaneous input for modulating inflammation. inflammatory reflex referred as the cholinergic antiinflammatory pathway regulates innate adaptive immunity, modulation of this by vagus stimulation (VNS) is effective various disease models, such rheumatoid arthritis bowel disease. Effectiveness VNS these models necessitates integration neural signals α7...
Development of proteinuria in lupus nephritis (LN) is associated with podocyte dysfunction. The NLRP3 inflammasome has been implicated the pathogenesis LN. purpose this study was to investigate whether activation involved development injury LN.A fluorescence-labeled caspase 1 inhibitor probe used detect inflammasomes podocytes derived from lupus-prone NZM2328 mice and renal biopsy tissues obtained patients MCC950, a selective NLRP3, treat mice. Proteinuria, ultrastructure, pathology were...
Abstract Lung CD11chigh dendritic cells (DC) are comprised of two major phenotypically distinct populations, the CD11bhigh DC and integrin αEβ7+ (CD103+ DC). To examine whether they functionally distinguishable, global microarray studies real-time PCR analysis were performed. Significant differences between types in chemokine mRNA expression found. is a secretory cell type highly expressed at least 16 homeostatic state, whereas CD103+ only 6. Intracellular staining lung including...
Abstract Although high dose exposure to inhaled cat allergen (Fel d 1) can cause a form of tolerance (modified Th2 response), the T cell mechanism for this phenomenon has not been studied. responses Fel 1 were characterized in both allergic (IgEpos) and modified (IgEnegIgGpos) responders as well serum Ab-negative controls (IgEnegIgGneg). stimulated levels IL-10 PBMC cultures from all individuals, with evidence Th1 cytokine skewing control subjects, respectively. Using overlapping peptides,...
Epithelial and endothelial injury a cascade of immune interstitial cell activation in the kidney lead to AKI. After mild moderate AKI, epithelium can regenerate restore function, yet little is known about endothelium during these repair processes. Sphingosine 1-phosphate receptor 1 (S1P1), G protein–coupled receptor, necessary for vascular homeostasis. Here, we used an inducible genetic approach mouse model ischemia–reperfusion (IRI), determine temporal effects S1P1 Deletion before IRI...
Effective clinical application of antiviral immunotherapies necessitates enhancing the functional state natural killer (NK) and CD8(+) T cells. An important mechanism for establishment viral persistence in liver is activation PD-1/PD-L1 inhibitory pathway. To examine role hepatic myeloid PD-L1 expression during infection, we determined magnitude quality immune responses by administering short-interfering RNA (siRNA) encapsulated lipidoid nanoparticles (LNP) mice. Our studies indicate that...
NLRP3 inflammasome regulates T cell responses. This study examined the roles of activation in regulation follicular helper (Tfh) cells during humoral response to dependent antigens and systemic lupus erythematosus (SLE).NLRP3 Tfh was studied B6, MRL/lpr NZM2328 mice SLE patients healthy controls using a fluorescence-labelled caspase-1 inhibitor probe. MCC950, selective NLRP3, used investigate relation between germinal centre (GC) reaction, Ab responses immunisation, autoantibody...
Abstract The liver maintains a tolerogenic environment to avoid unwarranted activation of its resident immune cells upon continuous exposure food and bacterially derived Ags. However, in response hepatotropic viral infection, the liver’s ability switch from hyporesponsive proinflammatory is mediated by select sentinels within parenchyma. To determine contribution hepatic dendritic (DCs) naive CD8+ T cells, we first characterized DC subsets murine liver. Liver DCs exhibit unique properties,...
Abstract The liver contains 2 transcriptionally distinct group 1 ILC subsets: CD49a+ ILC1s and CD49b+ NK cells. However, little is known about how ILCs contribute to hepatic immune responses. Therefore, we characterized murine liver-resident found that express high levels of the inhibitory receptor NKG2A localize near DCs in perivascular spaces surrounding portal triads. Upon viral infection, signaling ILCs, especially ILC1s, inhibits CXCL9 expression required for robust accumulation...
Abstract Glomerular damage mediated by glomerulus-infiltrating myeloid-derived cells is a key pathogenic event in lupus nephritis (LN), but the process poorly understood. Confocal microscopy of kidney sections and flow cytometry analysis glomerular from magnetic bead–purified glomeruli have identified leukocyte populations NZM2328 (NZM) lupus-prone mice with spontaneous chronic glomerulonephritis (GN) anti–glomerular basement membrane-induced nephritis. The occurrence major CD11b+F4/80−I-A−...
Significance Statement GSTM1 encodes a member of superfamily antioxidant enzymes, and highly prevalent deletion variant is associated with kidney disease progression in two human study cohorts. In this study, the authors demonstrate that Gstm1 knockout mice exhibit increased oxidative stress, injury, inflammation models CKD hypertension, loss parenchyma but not bone marrow–derived cells drives renal inflammation. Importantly, consumption broccoli powder or cruciferous vegetables was...
Abstract Activation and clonal expansion of the Ag-specific adaptive immune response in draining lymph node is essential to clearing influenza A virus infections. sufficient for clearance dependent on node’s architectural organization that maintained by stromal cells, chiefly fibroblastic reticular cells. During an analysis leptin receptor knockout (DB/DB) mice, we observed DB/DB mice have markedly reduced numbers cells at steady state. The reduction resulted abnormal diminished nodes under...
Pannexin 1 (Panx1) is a membrane-associated channel that, when activated, facilitates the release of small metabolites into extracellular environment. These signal as damage-associated molecular patterns (DAMP) and initiate inflammation. Upregulation activation Panx1 one early events during inflammatory injury. Animal models show that lack protective against acute kidney injury (AKI). How modulates AKI poorly understood. We utilized both in vivo vitro PANX1 overexpression to study...
Abstract Dendritic cells (DC) are the primary APC responsible for capture of allergens in airways and shuttling processed to draining lymph nodes where Ag presentation T cell activation take place. The mechanism this handling asthma is poorly understood. In addition, feasibility induction by DC priming has not been directly tested. report an model using intratracheally (i.t.) injected splenic was used address these issues. pulsed with a OVA or major MHC class II-restricted epitope peptide...
A pentahexosylceramide isolated from canine kidney and intestine was shown by enzyme hydrolyses, partial acid hydrolysis, permethylation analysis of the products gas-liquid chromatography-mass spectrometry, serological tests to be a Forssman hapten identical in structure with glycolipid horse spleen. The assigned N-acetylgalactosaminyl(α1→3) N-acetylgalactosaminyl(β1→3)galactosyl(α1→4)galactosyl(β1→4)glucosyl-(1→1)ceramide. In course this work, mass spectrometry employed elucidate structures...
Allergic rhinitis (AR) is a public health problem with high prevalence worldwide. We evaluated levels of specific IgE, IgA, and IgG4 antibodies to the Dermatophagoides pteronyssinus (Dpt) house dust mite its major allergens (Der p1 Der p2) in serum saliva samples from allergic nonallergic children. A total 86 children were analyzed, which 72 had AR 14 healthy Serum IgE serum/salivary Dpt, p1, p2 higher whereas IgA all positively correlated children, while negatively Dpt In conclusion,...