Background: Acute lung injury (ALI) is a type of severe pulmonary inflammatory disease with high rates morbidity and mortality. Now, an increasing number studies suggest that lncRNAs may act as key regulators the response play crucial role in pathogenesis many diseases. Our study firstly explored function underlying mechanism lncRNA metastasis-associated adenocarcinoma transcription 1 (MALAT1) regulating lipopolysaccharide (LPS)-induced ALI rats. Methods: The rats were constructed by...

Activation of nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome received substantial attention recently in inflammatory diseases. Macrophages contribute to allergic inflammation asthma. The present study was aimed investigate the effect NLRP3 on polarization macrophages. We utilized human primary monocytes and monocyte-derived macrophages expression components (NLRP3, apoptosis-associated specklike protein, caspase-1) its downstream cytokine interleukin-1β...

Noncoding RNAs (ncRNAs), primarily microRNAs and long ncRNAs, play important roles in lung cancer. However, the role of ncRNA (lncRNA)-X-inactive specific transcript (XIST) non-small-cell cancer (NSCLC) is unclear. The purpose this study was to explore biologic function potential mechanism XIST NSCLC progression.XIST, miR-137, Notch-1 expression were detected by quantitative real-time PCR (qRT-PCR). Levels proliferation- epithelial-mesenchymal transition (EMT)-related proteins assessed...

Long noncoding RNAs (lncRNAs) are involved in various human diseases. Recently, H19 was reported to be upregulated fibrotic rat lung and play a stimulative role bleomycin (BLM)-induced pulmonary fibrosis mice. However, its expression tissues mechanism of action remain unclear. Here, our observations showed that significantly microRNA 140 (miR-140) markedly reduced from idiopathic (IPF) patients transforming growth factor β1 (TGF-β1)-induced HBE A549 cells. Moreover, the negatively correlated...

Recently, several studies have been clarified that circular RNA (circRNA) was a vital regulatory gene of pulmonary hypertension (PH). Nevertheless, the action circRNA in PH not yet explored. This study to figure out biological function and potential molecular mechanism circSirtuin1 (SIRT1) PH. Construction rat model hypoxia artery smooth muscle cells (PASMC) performed, test circSIRT1/microRNA (miR)-145-5p/protein kinase-B3 (Akt3) conducted. The influence circSIRT1/miR-145-5p/Akt3 axis on...

This study aims to explore how microRNA-145 (miR-145) affects airway remodeling and cytokine expression by targeting epidermal growth factor receptor (EGFR) regulate mucin 5AC (MUC5AC).Mice alveolar epithelial cells (AECs) were divided into a control, blank, miR-145 mimics, mimic inhibitors, inhibitor si-EGFR inhibitors + group. Asthma mice models with induced an Ovalbumin (OVA) solution randomly normal, asthma, asthma or NC Airway degree histomorphology was measured using hematoxylin-eosin...

Background/Aims: Thymic stromal lymphopoietin (TSLP) is a cytokine that plays diverse roles in the regulation of immune responses. However, detailed understanding TSLP signaling pathway asthma remains elusive. In this study, we aimed to investigate role and its effect on airway inflammation remodeling. Methods: Forty Sprague Dawley (SD) rats were evenly classified into control, asthma, IgG2a mAb anti-TSLP groups. Ovalbumin (OVA)-induced models successfully established. Blood, bronchoalveolar...

Injury to airway smooth muscle (ASM) cells hallmarks the pathological progression of asthma, a chronic inflammatory disease. MicroRNAs (miRNAs) play essential roles in development asthma as well remodeling. Here we studied involvement miRNAs regulation autophagic survival ASM and disorder. We analyzed autophagy-associated factors LC3 Beclin-1 by RT-qPCR protein blotting purified from ovalbumin (OVA)-induced asthmatic mice. The biological activity miRNA targeting was explored bioinformatics...

Background Chronic kidney disease (CKD) is a growing public health problem with well-established risk factors. Other contributing factors, however, remain to be identified. Systemic inflammation in asthma plays significant role the development of other diseases. We therefore initiated study assess whether prevalence associated an increase CKD. Methods conducted retrospective cohort using data from 3015 patients aged 14 years and older who were registered followed up Asthma Control Study at...

Long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) plays a crucial role in non-small cell lung cancer (NSCLC). Nonetheless, regulatory effects of PVT1 on functions NSCLC cells remain blurry.Relative expression levels PVT1, miR-551b and FGFR1 mRNA tumor tissues were examined employing quantitative real-time polymerase chain reaction (qRT-PCR); CCK-8 BrdU assays utilized for measuring viability proliferation H1299 A549 cells; migration invasion detected deploying...

It has recently been shown that expression levels of tissue factor (TF) are high in the serum and peripheral blood mononuclear cells patients with asthma. However, whether TF impacts airway inflammation remodelling asthma remains unknown. The aim this study was to investigate effect using a house dust mite (HDM)-induced chronic model human bronchial epithelial (16HBE) cells. A constructed BALB/c mice by intranasal instillation HDM. Mice were treated short hairpin (shTF), features...

To explore the diagnostic value of joint detection soluble B7-H4 (sB7-H4) and carcinoembryonic antigen (CEA) in identifying malignant pleural effusion (MPE) from benign (BPE).A total 97 patients with specimens were enrolled The First Affiliated Hospital Zhengzhou University between June 2014 December 2015. All cases categorized into group (n = 55) 42) according to etiologies. Enzyme-linked immunosorbent assay was applied examine levels sB7-H4 meanwhile CEA concentrations detected by...

The aim of this study was to assess the incidence pulmonary thromboembolism (PTE) in patients with acute exacerbation chronic obstructive disease (AECOPD),and evaluate efficacy and safety low-dose urokinase (UK) thrombolysis therapy when treating hemodynamically stable AECOPD PTE (AECOPD-PTE). A total 419 patients, including 96 AECOPD-PTE, were enrolled. 30 AECOPD-PTE collected retrospectively, 66 prospectively divided into anticoagulation-only, UK standard-dose groups. Follow-up 1 year, we...

Long-chain polyunsaturated fatty acids (LCPUFAs) and the gut microbiota individually exert notable effects on asthma. However, it remains unknown whether LCPUFAs modulate microbial composition. Twenty-four male BALB/c mice were randomly assigned to control (saline), asthma (ovalbumin (OVA)), groups (OVA plus LCPUFAs). The enhanced pause value was observed decrease upon administration of in challenged with methacholine (12.5, 25, 50 g/mL). treatment decreased number eosinophils BALF compared...

Background Previous studies have suggested that pregnant women with pulmonary hypertension (PH) high maternal mortality. However, indexes or factors can predict death are lacking. Methods We retrospectively reviewed PH admitted for delivery from 2012 to 2020 and followed them over 6 months. The patients were divided into two groups according 10-day survival status after delivery. Predictive models predictors identified using four machine learning algorithms: naïve Bayes, random forest,...

Objective To study the effect of cigarette smoke extract on expression S100A2 in lung adenocarcinoma A549 cell. Methods Lung cells were treated by medium containing 3% extract, control group used without extract. cultured for 1, 2, 3 weeks collected separately, and expressions mRNA protein measured real-time PCR method western-blot separately. Results After one week, two three weeks, increased gradually, (1.729 ± 0.067) times, (2.420 0.106) times (3.368 0.105) to , difference...

Objective To study the expression of S100A2 lung cancer xenografts in nude mice. Methods Thirty mice were randomly divided into control group and treatment group. The model was established by intratracheal injection A549 cells group, treated with same volume normal saline. expressions mRNA protein measured real-time PCR method western-blot separately. Results One mouse died three successfully mice. relative tissues (2.126±0.076) times that difference significant(P<0.05)....

Objective To study the effect of 4-N-methylamine-nitroso-1-(3-pyridyl) acetone on proliferation, apoptosis lung adenocarcinoma A549 cell. Methods Lung cells were treated by medium containing 3% acetone. cultured for 1 weeks, 2 3 weeks collected separately, and cell proliferation was detected MTT method, observed Hoechst 33258 fluorescence staining. Results There no significant difference in absorbance control group (F=0.554, P=0.650). After one week, two three , 0.516±0.007,...

Objective To investigate the clinical characteristics of interstitial lung disease associated with cancer (ILD-LC). Methods Sixty-eight patients firstly detected on basis diagnosis (ILD) from January 2015 to 2018 in First Affiliated Hospital Zhengzhou University were allocated ILD-LC group; and 192 ILD same period set as group. The differences general data, tumor markers function between two groups retrospectively analyzed compared, group high-resolution computed tomography (HRCT)...