Xiaoxue Ren
A5091087629- Liver Disease Diagnosis and Treatment
- Immune cells in cancer
- Cancer Mechanisms and Therapy
- Phagocytosis and Immune Regulation
- FOXO transcription factor regulation
- Cancer Immunotherapy and Biomarkers
- Ferroptosis and cancer prognosis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pancreatic and Hepatic Oncology Research
- Diabetes and associated disorders
- Cytokine Signaling Pathways and Interactions
- Mechanisms of cancer metastasis
- RNA modifications and cancer
- interferon and immune responses
- Immune responses and vaccinations
- Cancer-related molecular mechanisms research
- Viral-associated cancers and disorders
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Colorectal Cancer Treatments and Studies
- Pediatric Hepatobiliary Diseases and Treatments
- 3D Printing in Biomedical Research
- Chemokine receptors and signaling
- Hepatocellular Carcinoma Treatment and Prognosis
The First Affiliated Hospital, Sun Yat-sen University
2021-2025
Sun Yat-sen University
2021-2025
Sun Yat-sen University Cancer Center
2022-2023
Objective Revealing the single-cell immune ecosystems in true versus de novo hepatocellular carcinoma (HCC) recurrences could help optimal development of immunotherapies. Design We performed 5’and VDJ RNA-sequencing on 34 samples from 20 recurrent HCC patients. Bulk RNA-sequencing, flow cytometry, multiplexed immunofluorescence, and vitro functional analyses were two validation cohorts. Results Analyses mutational profiles evolutionary trajectories paired primary using whole-exome sequencing...
Background and Aims: NASH-HCC is inherently resistant to immune checkpoint blockade, but its tumor microenvironment largely unknown. Approach Results: We applied the imaging mass cytometry construct a spatially resolved single-cell atlas from formalin-fixed paraffin-embedded tissue sections patients with NASH-HCC, virus-HCC (HBV-HCC HCV-HCC), healthy donors. Based on 35 biomarkers, over 750,000 individual cells were categorized into 13 distinct cell types, together expression of key...
The tumor microenvironment is distinctive in primary and secondary liver cancer. B cells represent an important component of immune infiltrates. Here, we demonstrated that are regulator hepatocellular carcinoma (HCC) colorectal cancer metastasis (CRLM) microenvironments. displayed distinct developmental trajectories HCC CRLM. Single-cell analysis revealed IgG+ plasma preferentially accumulated HCC, whereas IgA+ were enriched Mechanistically, recruited by tumor-associated macrophages via the...
Patients with biliary tract cancer (BTC) show different responses to chemotherapy, and there is no effective way predict chemotherapeutic response. We have generated 61 BTC patient-derived organoids (PDOs) from 82 tumors (74.4%) that similar histological genetic characteristics the corresponding primary tissues. tumor tissues enhanced stemness- proliferation-related gene expression by RNA sequencing can more easily form organoids. As expected, PDOs chemotherapies of gemcitabine, cisplatin,...
Liver metastasis is the leading cause of mortality in patients with colorectal cancer. Given significance both epithelial-mesenchymal transition (EMT) tumor cells and immune microenvironment cancer liver (CRLM), interplay between them could hold key for developing improved treatment options. We employed multiomics analysis 130 samples from 18 synchronous CRLM integrated external datasets to comprehensively evaluate interaction EMT metastasis. Single-cell RNA sequencing revealed distinct...
Targeted therapy and immunotherapy are transforming the treatment approach for intrahepatic cholangiocarcinoma (ICC). However, little is known about intertumor heterogeneity (ITH) of multifocal ICC its impacts on patient response to these treatments. We aimed characterize immunogenomic epigenomic guide decision making.We obtained 66 tumor samples from 16 patients with characterized immune using whole-exome sequencing, bulk single-cell RNA methylation microarray, multiplex immunostaining....
ABSTRACT Background Clonorchis sinensis infection is an important risk factor for intrahepatic cholangiocarcinoma (ICC). C. positive ( C.s +) ICC patients had much shorter overall survival (OS) compared with negative −) group. This study aims to explore the impact and underlying mechanism of on progression. Methods In this study, underwent surgery from two medical centers enrolled. RNA sequencing was used determine downstream activated pathways genes. Furthermore, we demonstrated potential...
Hepatitis B virus (HBV) infection is one of the most common risk factors for intrahepatic cholangiocarcinoma (ICC). However, there no direct evidence a causal relationship between HBV and ICC. In this study, we attempted to prove that ICC may originate from hepatocytes through pathological study involving tissue-derived organoids. The medical records tumor tissue samples 182 patients with after hepatectomy were collected. retrospectively analyzed explore prognostic factors. A microarray...
<p>Table S1. Clinical and pathological characteristics of CRLM patients</p>
<p>Table S2. CD45+ and CD45- cell numbers of each sample from CRLM patients</p>
<p>Figure S6. Pseudotime analysis of tumor cells by Palantir and CellRank.</p>
<p>Table S1. Clinical and pathological characteristics of CRLM patients</p>
<p>Figure S8. Fn14 expression is positively correlated with the metastatic ability of colon cancer cells.</p>
<p>Figure S9. Macrophage subclusters are important in the metastasis of CRLM.</p>
<div>Abstract<p>Liver metastasis is the leading cause of mortality in patients with colorectal cancer. Given significance both epithelial–mesenchymal transition (EMT) tumor cells and immune microenvironment cancer liver (CRLM), interplay between them could hold key for developing improved treatment options. We employed multiomics analysis 130 samples from 18 synchronous CRLM integrated external datasets to comprehensively evaluate interaction EMT metastasis. Single-cell RNA...
<p>Figure S5. Dynamic change of tumor cells from primary to metastatic lesions.</p>
<p>Figure S1. Cell annotations of mCRC scRNA-seq dataset.</p>
<p>Figure S4. Extraction and validation of tumor cell analysis in mCRC cohort.</p>
<div>Abstract<p>Liver metastasis is the leading cause of mortality in patients with colorectal cancer. Given significance both epithelial–mesenchymal transition (EMT) tumor cells and immune microenvironment cancer liver (CRLM), interplay between them could hold key for developing improved treatment options. We employed multiomics analysis 130 samples from 18 synchronous CRLM integrated external datasets to comprehensively evaluate interaction EMT metastasis. Single-cell RNA...
<p>Table S3. Marker genes of subcluster for each major cell type</p>
<p>Figure S8. Fn14 expression is positively correlated with the metastatic ability of colon cancer cells.</p>