A5085898289- Plant biochemistry and biosynthesis
- Natural product bioactivities and synthesis
- Cancer-related molecular mechanisms research
- Ginseng Biological Effects and Applications
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Plant and Fungal Interactions Research
- Gut microbiota and health
- Biological Activity of Diterpenoids and Biflavonoids
- Pharmacological Effects of Natural Compounds
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Synthetic Organic Chemistry Methods
- Oxidative Organic Chemistry Reactions
- Melanoma and MAPK Pathways
- interferon and immune responses
- Asymmetric Synthesis and Catalysis
- Computational Drug Discovery Methods
- Fungal Biology and Applications
- Protein Kinase Regulation and GTPase Signaling
- Cyclopropane Reaction Mechanisms
- Sesquiterpenes and Asteraceae Studies
- Viral Infections and Vectors
- Bone and Dental Protein Studies
- Plant Ecology and Soil Science
Changchun University of Chinese Medicine
2022-2025
Shanghai Public Security Bureau
2025
Takeda (United States)
2016-2024
National Chung Hsing University
2024
National Taiwan University
2012-2023
Agricultural Biotechnology Research Center, Academia Sinica
2022
Ningxia Medical University
2021
Institute of Zoology
2021
Chinese Academy of Sciences
2021
Fudan University
2021
Aminoacyl transfer RNA (tRNA) synthetases (aaRSs) are attractive drug targets, and we present class I II aaRSs as previously unrecognized targets for adenosine 5'-monophosphate-mimicking nucleoside sulfamates. The target enzyme catalyzes the formation of an inhibitory amino acid-sulfamate conjugate through a reaction-hijacking mechanism. We identified 5'-sulfamate broad-specificity compound that hijacks range ML901 specific reagent single aaRS in malaria parasite Plasmodium falciparum,...
Abstract Our recent studies demonstrate that the focal adhesion protein Kindlin-2 is critical for chondrogenesis and early skeletal development. Here, we show deleting from osteoblasts using 2.3-kb mouse Col1a1-Cre transgene minimally impacts bone mass in mice, but 10-kb Dmp1-Cre transgene, which targets osteocytes mature osteoblasts, results striking osteopenia mice. loss reduces osteoblastic population increases osteoclastic adipocytic populations microenvironment. upregulates sclerostin...
Abstract β-Cell dysfunction and reduction in β-cell mass are hallmark events of diabetes mellitus. Here we show that β-cells express abundant Kindlin-2 deleting its expression causes severe diabetes-like phenotypes without markedly causing peripheral insulin resistance. Kindlin-2, through C-terminal region, binds to stabilizes MafA, which activates expression. loss impairs secretion primary human mouse islets vitro mice by reducing, at least part, Ca 2+ release β-cells. GSK-3β downregulates...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTFormation of Tetrahydrofuran from Homoallylic Alcohol via a Tandem Sequence: 2-Oxonia [3,3]-Sigmatropic Rearrangement/Cyclization Catalyzed by In(OTf)3Teck-Peng Loh, Qi-Ying Hu, and Li-Ting MaView Author Information Department Chemistry, National University Singapore 3 Science Drive 3, 117543, Cite this: J. Am. Chem. Soc. 2001, 123, 10, 2450–2451Publication Date (Web):February 16, 2001Publication History Received28 November 2000Published...
Stimulator of Interferon Genes (STING) plays an important role in innate immunity by inducing type I interferon production upon infection with intracellular pathogens. STING activation can promote increased T-cell and inflammation the tumor microenvironment, resulting antitumor immunity. Natural synthetic cyclic dinucleotides (CDNs) are known to activate STING, several CDN molecules being investigated clinic using intratumoral administration route. Here, we describe identification agonist...
As a major staple food, maize (Zea mays) is critical to food security. Shifting environmental pressures increasingly hamper crop defense capacities, causing expanded harvest loss. Specialized labdane-type diterpenoids are key components of chemical and ecological adaptation. Labdane diterpenoid biosynthesis most commonly requires the pairwise activity class II I diterpene synthases (diTPSs) that convert central precursor geranylgeranyl diphosphate into distinct scaffolds. Two diTPSs, ANTHER...
Mammalian focal adhesion proteins Pinch1 and Pinch2 regulate integrin activation cell–extracellular matrix migration. Here, we show that deleting in osteocytes mature osteoblasts using the 10-kb mouse Dmp1-Cre globally (double KO; dKO) results severe osteopenia throughout life, while ablating either gene does not cause bone loss, suggesting a functional redundancy of both factors bone. Pinch deletion generates signals inhibit osteoblast formation. Pinch-deficient conditioned media from dKO...
Antrodia cinnamomea, a precious, host-specific brown-rot fungus that has been used as folk medicine in Taiwan for centuries is known to have diverse bioactive compounds with potent pharmaceutical activity. In this study, different fermentation states of A. cinnamomea (wild-type fruiting bodies and liquid cultured mycelium) were sequenced using the next-generation sequencing (NGS) technique. A 45.58 Mb genome encoding 6,522 predicted genes was obtained. High quality reads assembled into total...
Inhibition of mutant B-Raf signaling, through either direct inhibition the enzyme or MEK, substrate Raf, has been demonstrated preclinically to inhibit tumor growth. Very recently, treatment melanoma patients with a selective inhibitor resulted in promising preliminary evidence antitumor activity. This article describes design and optimization tetrahydronaphthalene-derived compounds as potent inhibitors Raf pathway vitro vivo. These possess good pharmacokinetic properties rodents growth...
Autophagy is postulated to be required by cancer cells survive periods of metabolic and/or hypoxic stress. ATG7 the E1 enzyme that for activation Ubl conjugation pathways involved in autophagosome formation. This article describes design and optimization pyrazolopyrimidine sulfamate compounds as potent selective inhibitors ATG7. Cellular levels autophagy markers, LC3B NBR1, are regulated following treatment with these compounds.
Antodia cinnamomea, a precious brown-rot fungus endemic to Taiwan, has pharmaceutical applications due its diverse array of metabolites. The terpenoids found in A. cinnamomea contribute most important bioactivities. We identified several terpenoid compounds and revealed that their content mycelium fruiting body were significantly different. Using next-generation sequencing an in-house transcriptome database, we terpene synthase (TPS) candidates. After sequence analysis functional...
Recent advances in sequencing technology have opened a new era RNA studies. Novel types of RNAs such as long non-coding (lncRNAs) been discovered by transcriptomic and some lncRNAs found to play essential roles biological processes. However, only limited information is available for Drosophila melanogaster, an important model organism. Therefore, the characterization identification D. melanogaster area research. Moreover, there increasing interest use ChIP-seq data (H3K4me3, H3K36me3 Pol II)...
Abstract Bacterial polyynes are highly active natural products with a broad spectrum of antimicrobial activities. However, their detailed mechanism action remains unclear. By integrating comparative genomics, transcriptomics, functional genetics, and metabolomics analysis, we identified unique polyyne resistance gene, masL (encoding acetyl-CoA acetyltransferase), in the biosynthesis gene cluster antifungal (massilin A 1 , massilin B 2 collimonin C 3 D 4 ) Massilia sp. YMA4. Crystallographic...
Patients with a spleen-qi deficiency often exhibit dysfunction in the metabolic system. Metabolites are considered most direct reflection of individual physiological and pathological conditions represent attractive candidates to provide deep insights into disease phenotypes. This study examines potential therapeutic mechanism wild ginseng on through analysis serum urine metabolomics using rapid-resolution liquid chromatography coupled quadrupole-time-of-flight mass spectrometry. The reasons...
Spleen-qi deficiency syndrome, a common weakness syndrome in traditional Chinese medicine, results from insufficient spleen-qi levels. For centuries, ginseng has been relied upon as medicine to treat syndrome. Until now, the mechanism feature of treating temper through intestinal bacteria and short-chain fatty acid (SCFA) metabolites not fully elucidated.This study established rat model via multi-factor compound modeling that involved fatigue injury controlled diet. The content SCFAs between...
Disease vectors such as mosquitoes and ticks play a major role in the emergence re-emergence of human animal viral pathogens. Compared to mosquitoes, however, much less is known about antiviral responses ticks. Here we showed that Asian longhorned ( Haemaphysalis longicornis ) produced predominantly 22-nucleotide virus-derived siRNAs (vsiRNAs) response severe fever with thrombocytopenia syndrome virus (SFTSV, an emerging tick-borne virus), Nodamura (NoV), or Sindbis (SINV) acquired by blood...
Taiwania cryptomerioides is a monotypic gymnosperm species, valued for the high decay resistance of its wood. This durability has been attributed to abundance terpenoids, especially major diterpenoid metabolite ferruginol, with antifungal and antitermite activity. Specialized metabolism in gymnosperms primarily recruits bifunctional class-I/II diterpene synthases (diTPSs), whereas monofunctional class-II class-I enzymes operate angiosperms. In this study, we identified previously...
The gut microbiota contribute significantly to the immune system. Low-medium polarity ginsenosides from wild ginseng (LWG) have potential immunomodulatory effects. However, how LWG regulates enhance immunity remains unclear. To explore interaction between microbes and metabolites mediating LWG's effects, this study examined impact on splenocytes CTX-induced immunosuppressed mice. Metabolomic metagenomic analyses were conducted in vivo mechanism by which immunity.
[reaction: see text] Poor stereoselectivity caused by the involvement of side reaction pathways was observed in In(OTf)(3)-catalyzed allyl-transfer (R = electron-withdrawing group). Subsequently, it found that employment triflic acid (TFA) as catalyst could successfully suppress and, hence, achieve high stereoselectivity.
Abstract Taiwania ( cryptomerioides Hayata) is an indigenous conifer species of Taiwan. Various secondary metabolites with diverse bioactivities have been identified, and lignans are especially abundant in the heartwood (hW). In present study, wood this was separated to cambium (Cam), sapwood (sW), transition zone (TZ) hW their transcriptomes were sequenced. Three pinoresinol-lariciresinol reductases PLR s; designated TcPLR1 , TcPLR2.2 TcPLR3 ), which responsible for lignan biosynthesis,...