A5040453415- Pancreatic function and diabetes
- Liver physiology and pathology
- Pluripotent Stem Cells Research
- Organ Transplantation Techniques and Outcomes
- Diabetes Management and Research
- Tissue Engineering and Regenerative Medicine
- Diabetes and associated disorders
- Xenotransplantation and immune response
- CRISPR and Genetic Engineering
- Estrogen and related hormone effects
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Pain Mechanisms and Treatments
- Supramolecular Self-Assembly in Materials
- Pancreatitis Pathology and Treatment
- Cannabis and Cannabinoid Research
- Genetics and Neurodevelopmental Disorders
- 3D Printing in Biomedical Research
- Metabolism, Diabetes, and Cancer
- Apelin-related biomedical research
- Acute Kidney Injury Research
- Ion channel regulation and function
- Histone Deacetylase Inhibitors Research
- Vitamin K Research Studies
- Breast Cancer Treatment Studies
Universidad de Guadalajara
2016-2025
Autonomous University of Aguascalientes
2017
Oklahoma State University Center for Health Sciences
2011
Okayama University
2005-2010
Artificial bones have often used for bone regeneration due to their strength, but they cannot provide an adequate environment cell penetration and settlement. We therefore attempted explore various materials that may allow the cells penetrate engraft in defects. PuraMatrix is a self-assembling peptide scaffold produces nanoscale allowing both cellular engraftment. The objective of this study was investigate effect on mouse defect model calvaria. Matrigel as control. expression bone-related...
Bone-derived proteins, including carboxylated osteocalcin (cOC), are thought to play a role in cardiovascular and metabolic health. cOC is recognized for its strong affinity calcium hydroxyapatite possible involvement vascular calcification lipid metabolism. Although the undercarboxylated form of (ucOC) has been widely researched, connections between risk markers, such as mean arterial pressure (MAP), pulse (PP), atherogenic index, still not well understood. This cross-sectional study...
Human embryonic stem (hES) cells have recently been studied as an attractive source for the development of a bioartificial liver (BAL). Here we evaluate differentiation capacity hES into hepatocytes. were subjected to suspension culture 5 days, and then cultured onto poly-amino-urethane (PAU)-coated, nonwoven polytetrafluoroethylene (PTFE) fabric in presence fibroblast growth factor-2 (bFGF) (100 ng/ml) 3 with deleted variant hepatocyte factor (dHGF) 1% dimethyl sulfoxide (DMSO) 8 finally...
Islet transplantation is associated with an elevated rate of early graft failure. The isolation process leads to structural and functional abnormalities. reestablishment the cell–matrix relationship important modulate survival function islets. Thus, we evaluated effect human fibronectin (hFN) self-assembling peptide nanofiber (SAPNF) in ability support islet vitro after into streptozotocin (STZ)-induced diabetic severe combined immunodeficiency (SCID) mice. Human isolated islets were...
AIMTo determine a potential relationship between serum undercarboxylated (ucOC) concentration and cardiovascular risk factors in type 2 diabetes (T2D) patients healthy subjects (HS).
Human embryonic stem (hES) cells have the ability to differentiate into a variety of different cell lineages and potentially provide source differentiated for many therapeutic uses. Here we investigated an efficient method hepatic differentiation from hES cells. A human ES line, KhES-1, was used maintained by nonfeeder method. KhES-1 were cultured 5 days in presence activin (50 ng/ml) then treated with deleted variant hepatocyte growth factor (dHGF) at 0, 100, or 500 ng/ml 7 days. The...
Freshly isolated porcine hepatocytes are a very attractive cell source in the cell-based therapies to treat liver failure because of unlimited availability. However, due loss hepatocyte functions vitro, there is need develop functional culture system keep cells metabolically active. Here we compared effect self-assembling peptide nanofiber (SAPNF) as an extracellular matrix (ECM) with collagen type I on metabolic and secretion activities following isolation. Isolated were cultured SAPNF I....
Treatment of diabetic patients by pancreatic islet transplantation often requires the use islets from two to four donors produce insulin independence in a single recipient. Following isolation and transplantation, are susceptible apoptosis, which limits their function probably long-term graft survival.To address this issue, we examined effect cell-permeable apoptosis inhibitor pentapeptide Val-Pro-Met-Leu-Lys, V5, on mouse model.V5 treatment upregulated expression anti-apoptotic proteins...
Islet transplantation has become a widely accepted therapeutic option for selected patients with type 1 diabetes mellitus. However, in order to achieve insulin independence great number of islets are often pooled from 2 4 pancreata donors. Mostly, it is due the massive loss immediately after transplant. The endothelium plays key role function native and during revascularization process islet transplantation. if delayed occurs, even remaining will also undergo cell death late graft...
Construction of a safe and functional bioartificial pancreas (BAP) that provides an adequate environment for islet cells may be important approach to treating diabetic patients. Various types BAP devices have been developed, but most them involve extravascular implantation islets in microcapsules or diffusion chambers. These poor diffusive exchange between the blood, often rupture. To overcome these problems, we developed new type composed polyethylene-vinyl alcohol (EVAL) hollow fibers are...
Development of an efficient preculture system islets is ideal. Toward that goal, we constructed a human pancreatic islet-derived fibroblast cell line MNNK-1 for source as coculture freshly isolated to maintain islet functions. Human cells were nucleofected with plasmid vector pYK-1 expressing simian virus 40 large T antigen gene (SV40T) and hygromycin resistance (HygroR). One the transduced lines, MNNK-1, was established served feeder in mouse, rat, pig islets. Morphology, viability,...
Considering the scarcity of donor livers, it is extremely important to establish a functional culture method for isolated hepatocytes. As tool maintaining hepatocyte functions in vitro, dHGF, variant HGF (hepatocyte growth factor) with deletion five amino acids, attracted our attention because less cytotoxic compared HGF.We evaluated growth, albumin production, metabolizing abilities ammonia, lidocaine, and diazepam human hepatocytes presence dHGF (10-1000 ng/ml). The gene expression liver...
Fibroblast growth factor (FGF)-2 has been recognized to be a key element involved in angiogenesis and putative stem cell-mediated islet regeneration. However, the usefulness of FGF-2 an transplantation setting not yet explored. We therefore evaluated effect on both culture transplantation. Isolated islets were cultured presence 100 ng/ml for week then glucose-responding insulin secretion contents measured. Gelatinized (100 ng), which allowed controlled release FGF-2, was used...
Background. Acute liver failure (ALF) is a serious condition that has high mortality rate. Construction of an efficient culture and transplantation engineering system hepatic tissue important approach to treat patients suffering from ALF provide short-term support until the damaged spontaneously recovers or donor becomes available for transplantation. Here, we evaluate construction engineered (EHT) using primary isolated hepatocytes cultured onto polyaminourethane (PAU)-coated, nonwoven...
Background. Because hepatocyte transplantation has been considered to be an attractive method treat acute liver failure (ALF), efficient recovery of hepatocytes and maintenance differentiated functions is extreme importance. We here report the usefulness antiapoptotic pentapeptide V5, composed Val-Pro-Met-Leu-Lys, in monkey cultures. Methods. evaluated albumin production, metabolizing abilities ammonia, lidocaine, diazepam cultured with V5. The protein expression apoptosis-associated...
Locomotion speed changes appear following hippocampal injury. We used a penetrating brain injury mouse model to analyze other kinematic changes. found significant decrease in locomotion both open-field and tunnel walk tests. described new quantitative method that allows us compare the displacement curves between mice steps. In walk, we marked with indelible ink on knee, ankle, metatarsus of left right hindlimbs evaluate every step. Animals damage exhibit slower hindlimbs. contrast, cortical...