A5082617727- Occupational and environmental lung diseases
- Medical Imaging and Pathology Studies
- Tissue Engineering and Regenerative Medicine
- RNA modifications and cancer
- Cancer Research and Treatments
- Lung Cancer Treatments and Mutations
- Virus-based gene therapy research
- Cancer-related Molecular Pathways
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- PI3K/AKT/mTOR signaling in cancer
- Lung Cancer Research Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- RNA Interference and Gene Delivery
- Neonatal Respiratory Health Research
- Congenital Diaphragmatic Hernia Studies
- Cancer Genomics and Diagnostics
- FOXO transcription factor regulation
- Sarcoma Diagnosis and Treatment
- Cell death mechanisms and regulation
- Epigenetics and DNA Methylation
- Heat shock proteins research
- Cancer Cells and Metastasis
Kawasaki Medical School
2016-2025
Okayama University
2005-2023
Kawasaki Medical School Hospital
2014
Okayama Rosai Hospital
2011
NYU Langone Health
2011
Okayama Shoka University
2010-2011
University of California, Riverside
2003-2009
Catholic University of Korea
2009
Shigei Medical Research Institute
2009
Cincinnati Children's Hospital Medical Center
2007-2008
Mucinous adenocarcinoma of the lung is a subtype highly invasive pulmonary tumors and associated with decreased or absent expression transcription factor NK2 homeobox 1 (NKX2-1; also known as TTF-1). Here, we show that haploinsufficiency Nkx2-1 in combination oncogenic KrasG12D, but not EGFRL858R, caused transgenic mice were phenotypically similar to human mucinous adenocarcinomas. Gene patterns distinguished tumor goblet (mucous) cells from nontumorigenic airway intestinal cells. Expression...
Malignant pleural mesothelioma (MPM) is an aggressive tumor with a dismal prognosis. Unlike other malignancies, TP53 mutations are rare in MPM. Recent studies have showed that altered expression of microRNA (miRNA) observed human malignant tumors. In this study, we investigated the alterations miR-34s, direct transcriptional target TP53, and role miR-34s on pathogenesis MPM.Aberrant methylation were examined MPM cell lines miR-34b/c was transfected to cells estimate protein expression,...
Research Article2 March 2017Open Access Source DataTransparent process Gene signature driving invasive mucinous adenocarcinoma of the lung Minzhe Guo Perinatal Institute, Divisions Neonatology, and Pulmonary Biology, Cincinnati Children's Hospital Medical Center University College Medicine, Cincinnati, OH, USA Department Electrical Engineering Computing Systems, Search for more papers by this author Koichi Tomoshige Michael Meister Translational Unit, Thoraxklinik at Heidelberg, Lung...
Despite high expectations for lung tumoroids, they have not been applied in the clinic due to difficulty of their long-term culture. Here, however, using AO (airway organoid) media developed by Clevers laboratory, we succeeded generating 3 tumoroid lines culture (>13 months) from 41 cancer cases (primary or metastatic). Use nutlin-3a was key selecting tumoroids that harbor mutant p53 order eliminate normal epithelial organoids. Next-generation sequencing (NGS) analysis indicated each carried BRAF
Background. Cholangiocytes perform an essential role in important pathophysiologic functions the liver. Establishment of a human cholangiocyte line facilitates advances research and clinical applications for cell therapies. Here, we describe immortalization cholangiocytes using serial transfection simian virus 40 large T (SV40T) followed by telomerase reverse transcriptase (hTERT). Methods. SV40T-transduced liver OUMS-21 cells were superinfected with retroviral vector SSR#197 encoding hTERT...
Midkine (MDK) is a heparin-binding growth factor that highly expressed in many malignant tumors, including lung cancers. MDK activates the PI3K pathway and induces anti-apoptotic activity, turn enhancing survival of tumors. Therefore, inhibition considered potential strategy for cancer therapy. In present study, we demonstrate novel small molecule compound (iMDK) targets MDK. iMDK inhibited cell MDK-positive H441 adenocarcinoma cells harbor an oncogenic KRAS mutation H520 squamous cells,...
Background and Aims. Liver endothelial cells (LECs) perform an essential role in important pathophysiologic functions the liver. Establishment of a human LEC line facilitates advances research. Here, we present immortalization LECs using retroviral gene transfer simian virus 40 large T antigen (SV40T) telomerase reverse transcriptase (hTERT). We also demonstrate excision SV40T hTERT with TAT-mediated Cre/loxP recombination subsequent cell sorting. Methods. First, were transduced vector...
Abstract Since the SOX2 amplification was identified in lung squamous cell carcinoma (lung SCC), transcriptional downstream targets have been actively investigated; however, such are often line specific. Here, order to identify highly consensus genes SCC cells, we used RNA-seq data from 178 specimens (containing tumor and tumor-associated cells) analyzed correlation between previously-reported -controlled SCC. In addition, another dataset 105 non-small cancer lines (NSCLC; including 4 lines)...
In the past few years, several types of artificial transcriptional activator, based on CRISPR-Cas9, have been developed and refined. Of these, in synergistic activation mediator SunTag systems, effector proteins, expressed trans, can be recruited to target sites via MS2 RNA-binding system GCN4-scFv antibody system, respectively. Here, we report a strong achieved by fusing GCN4 repeat coat protein accumulate numbers activators, fused scFv antibodies. By targeting CDH1 gene, show that our...
Pulmonary large-cell neuroendocrine carcinoma (LCNEC), a disease with poor prognosis, is classified as pulmonary high-grade carcinoma, along small-cell lung cancer. However, given its infrequent occurrence, only limited number of preclinical models have been established. Here, we established three LCNEC tumoroids for long-term culture. Whole-exome sequencing revealed that these inherited genetic mutations from their parental tumors; two were (S-LCNEC) and one non-small cell (N-LCNEC)....
TP63 encodes TAp63, which is functionally similar to the tumor suppressor TP53, and ΔNp63, lacks transcription-activating domain of TAp63 appears potently oncogenic in squamous cell carcinomas (SCCs). In this study, we developed an integrated CRISPR interference (CRISPRi) system selectively suppress ΔNp63 (CRISPRiΔNp63). We engineered CRISPRi using tandemized guide RNA expression cassettes that targeted 50 100 bp downstream transcription start site combination with inactivated Cas9 linked...
The liver is exposed to a wide variety of toxic agents, many which damage DNA and result in increased levels the tumour suppressor protein p53. We have previously shown that p53 inhibits transactivation function HNF (hepatocyte nuclear factor) 4α1, receptor known be critical for early development differentiation. In present study we demonstrate also down-regulates expression human HNF4α gene via proximal P1 promoter. Overexpression wild-type down-regulated endogenous both mRNA Hep3B cells....
Esophageal squamous cell carcinoma (ESCC) remains one of the most aggressive cancers with poor prognosis regardless a several reports that indicate better therapeutic efficacy using some new chemotherapeutic agents. Recent drug development has contributed to an improved specificity suppress mTOR activity by which many types malignancies can be explosively progressed. Temsirolimus (CCI-779, TricelTM) is recently synthesized analogs rapamycin and provided outcomes for patients renal carcinoma....
The microRNA-34s (miR-34s) have p53 response elements in their 5ʼ-flanking regions and demonstrate tumor-suppressive functions. In malignant pleural mesothelioma (MPM), we previously reported that expression of miR-34b miR-34c (miR-34b/c) was frequently downregulated by methylation MPM cell lines primary tumors. forced overexpression miR-34b/c showed significant antitumor effects with the induction apoptosis cells. this study, examined vivo using adenovirus vector on MPM. We subcutaneously...