A5023372047- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Parasitic infections in humans and animals
- Urinary Tract Infections Management
- Escherichia coli research studies
- Reproductive Biology and Fertility
- Vibrio bacteria research studies
- Parasitic Infections and Diagnostics
- Reproductive Physiology in Livestock
- Ovarian function and disorders
- Animal Behavior and Welfare Studies
- Genetic Syndromes and Imprinting
- Pluripotent Stem Cells Research
- Genetic and Kidney Cyst Diseases
- Reproductive System and Pregnancy
- Hedgehog Signaling Pathway Studies
- Sperm and Testicular Function
- Bacterial biofilms and quorum sensing
- Wnt/β-catenin signaling in development and cancer
- Indoor Air Quality and Microbial Exposure
- Clostridium difficile and Clostridium perfringens research
- MicroRNA in disease regulation
- Enterobacteriaceae and Cronobacter Research
- Neuroendocrine regulation and behavior
- Virus-based gene therapy research
Institut Pasteur de Montevideo
2013-2025
Instituto de Investigaciones Biológicas Clemente Estable
2003-2016
Institut Pasteur
2010-2015
Infertility affects a considerable number of couples at reproductive age, with an incidence 10-15%. Approximately 25% cases are classified as idiopathic infertility. Often, errors during the meiotic stage appear to be related A crucial component first prophase is synaptonemal complex (SC), which plays fundamental role in homologous chromosome pairing and recombination. In many studies infertile patients, mutations affecting SC-coding genes have been identified. The generation humanized...
Proteus mirabilis is one of the most common etiological agents complicated urinary tract infections, especially those associated with catheterization. This related to ability P. form biofilms on different surfaces. pathogen encodes 17 putative fimbrial operons, highest number found in any sequenced bacterial species so far. The present study analyzed role four fimbriae (MR/P, UCA, ATF and PMF) biofilm formation using isogenic mutants. Experimental approaches included migration over catheter,...
Proteus mirabilis expresses different types of fimbriae simultaneously. Several fimbrial have been described and their role in the colonization urinary tract is under study. Previously, P. (PMF) shown to be associated with bacterial lower but not kidneys. In this study, a pmfA mutant was generated used several vivo vitro studies. Two infection models mouse two assays adhesion uroepithelial cells were performed. Expression PmfA collection strains sources also assessed. The results here...
Proteus mirabilis has been described as an aetiological agent in a wide range of infections, playing important role urinary tract infections (UTIs). In this study, collection P. isolates obtained from clinical and non-clinical sources was analysed order to determine possible correlation between origin, virulence factors vivo infectivity. Isolates were characterized vitro, assessing several properties that had previously associated with uropathogenicity. Swarming motility, urease production,...
Proteus mirabilis is an important uropathogen that can cause complicated urinary tract infections (UTI). It produces several types of fimbriae, including mannose-resistant Proteus-like (MR/P) fimbriae and P. (PMF). Previously, we determined these affect the ability to colonize tract. The objective this study was analyse effect simultaneous lack MR/P PMF in UTI pathogenesis. A double mutant lacking both generated by allelic replacement mutagenesis. This characterized genetically...
Cystic echinococcosis is caused by the larval stages (hydatids) of cestode parasites belonging to species cluster Echinococcus granulosus sensu lato, with E. stricto being main infecting species. Hydatids are bladderlike structures that attain large sizes within various internal organs livestock ungulates and humans. protected massive acellular laminated layer (LL), composed mainly mucins. Parasite growth requires LL turnover, abundant LL-derived particles found at infection sites in...
Emerging evidence suggests that immune receptors may participate in many aging-related processes such as energy metabolism, inflammation, and cognitive decline. CD300f, a TREM2-like lipid-sensing receptor, is an exceptional receptor it integrates activating inhibitory cell-signaling pathways modulate efferocytosis, microglial metabolic fitness. We hypothesize CD300f can regulate systemic ultimately healthy lifespan. closely followed several cohorts of two strains CD300f-/- WT mice both sexes...
Rodents used in biomedical research are maintained behind barriers to exclude microbial contaminants. Several check points have be monitored eliminate the potential of introducing adventitious agents into facility. Microbiological monitoring a mouse facility environment enables evaluate efficiency sanitization and cleaning procedures, air quality, technician good practices. At our SPF facility, we implemented an environmental microbiological program based sedimentation swabbing, inexpensive...
Background: Transgenesis by microinjection has been widely used for the generation of different mouse models.Different variables procedure may critically affect efficiency process.A DNA construction that carries CXCL2 promoter gene and firefly luciferase to optimize aspects procedure.Three concentrations (0.5, 1.0 4.0 ng/µl) microinject a total 1981 zygotes tested.Intact/injected embryos, pregnancy birth rate, survival pups 7 days after birth, number transgenic overall was registered...
The aim of this study was to evaluate the effect an environmental enrichment (EE) plan on reproductive performance Swiss Webster mice and their female offspring used as recipients for embryo transfer. A total 54 breeder 60 F1 females, foster mothers, were allocated in two experimental groups receive or not EE physical well-being. Reproductive outcomes trios such birth rate pup number, litter size, pups' weight at weaning, interlitter interval time first analyzed. Environmental significantly...
SPATS1 (spermatogenesis-associated, serine-rich 1) is an evolutionarily conserved, testis-specific protein that differentially expressed during rat male meiotic prophase. Some reports have suggested a link between underexpression/mutation and human pathologies such as infertility testicular cancer. Given the absence of functional studies, we generated Spats1 loss-of-function mouse model using CRISPR/Cas9 technology. The phenotypic analysis showed no overt phenotype in -/- mice, with both...
Abstract The protein Deleted in Breast Cancer 1 (DBC1), a regulator of several transcription factors and epigenetic modulators, plays determinant roles metabolism regulation, obesity aging-related processes. Knockout mice for Dbc1, develop morbid but are protected against liver steatosis, insulin resistance atherosclerosis. We have proposed that this healthy phenotype was mainly due to the expansion adipose tissue, avoiding free-fatty acid spillover metabolic damage peripheral tissues. To...
Microinjection of CRISPR/Cas9 requires the availability zygotes that implies animal breeding, superovulation schemes, and embryo collection. Vitrification may allow having ready-to-use embryos to temporally dissociate workload production from microinjection. In this study, fresh (F group) or vitrified (V were microinjected with system test hypothesis could be a suitable source for Experiment 1 ( in vitro evaluation), B6D2F1/J cultured until blastocyst stage. Embryo survival cleavage rates...
CCDC28B (coiled-coil domain-containing protein 28B) was identified as a modifier in the ciliopathy Bardet-Biedl syndrome (BBS). Our previous work cells and zebrafish showed that plays role regulating cilia length mechanism is not completely understood. Here we report generation of Ccdc28b mutant mouse using CRISPR/Cas9 ( mut ). Depletion resulted mild phenotype. animals i) do present clear structural affectation, although did observe defects density some tissues, ii) reproduce normally, iii)...
The objective was to evaluate the influence of colony aging in a Swiss Webster (SW) outbred stock used as recipients for embryo transfer. In first study, retrospective analysis performed throughout several generations during 38-month period 2,398 embryos transferred 108 SW recipients. A decrease percentage live pups from found at end period. Impairment occurred due incidence maternal cannibalism that increased 0% 67-100% (P<0.05), while pregnancy rate (pregnant/transferred recipients) and...
Abstract Cystic echinococcosis is caused by the larval stages (hydatids) of cestode parasites belonging to species cluster Echinococcus granulosus sensu lato , with E. stricto being main infecting species. Hydatids are bladder-like structures that attain large sizes within various internal organs livestock ungulates and humans. protected massive acellular laminated layer (LL), composed mainly mucins. Parasite growth requires LL turnover, abundant LL-derived particles found at infection sites...