A5045875574- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Cell Image Analysis Techniques
- HER2/EGFR in Cancer Research
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Cellular Mechanics and Interactions
- Advanced Thermoelectric Materials and Devices
- Neuroscience and Neural Engineering
- Barrier Structure and Function Studies
- Gene expression and cancer classification
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Phytochemistry Medicinal Plant Applications
- Radiopharmaceutical Chemistry and Applications
- Plant-Derived Bioactive Compounds
- Mathematical Biology Tumor Growth
- Nutrition, Genetics, and Disease
- BRCA gene mutations in cancer
- Nanofabrication and Lithography Techniques
- Electrowetting and Microfluidic Technologies
Terasaki Foundation
2022-2025
Massachusetts Institute of Technology
2020-2024
Phenikaa University
2023
Phenikaa (Vietnam)
2023
École Polytechnique Fédérale de Lausanne
2016-2019
Hanoi Medical University
2019
Icahn School of Medicine at Mount Sinai
2017
Nanyang Technological University
2015
Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics MVN is investigated a robust two-step strategy generate established.
Desmoplasia in breast cancer leads to heterogeneity physical properties of the tissue, resulting disparities drug delivery and treatment efficacy among patients, thus contributing high disease mortality. Personalized vitro models hold great promise for high-throughput testing therapeutic strategies normalize aberrant microenvironment a patient-specific manner. Here, tumoroids assembled from cell lines (MCF7, SKBR3, MDA-MB-468) patient-derived tumor cells (TCs) cultured microphysiological...
The application of three-dimensional (3D) cell cultures such as spheroids and organoids is growing in popularity both academia industry. However, morphology the 3D architecture remains remarkably understudied. Here, we introduce an open-access Spheroid Light Microscopy Image Atlas (SLiMIA) that can serve a training set for studies cultures. We provide images with variety metadata: 9 microscopes, 47 lines, 8 culture media, 4 spheroid formation methods multiple seeding densities; totalling...
ABSTRACT Background Embolization is a well-established treatment modality for liver cancer. However, traditional embolization agents are limited by inefficient delivery and aggregation in blood vessels. Novel shear-thinning hydrogels (STH) have been developed to address the need safer more effective local of embolic therapeutics. Objective We aim evaluate efficacy novel such as STH using human-relevant vitro model that recapitulates human hepatocellular carcinoma capillary networks. Methods...
Abstract Endothelial programmed death‐ligand 1 (PD‐L1) expression is higher in tumors than normal tissues. Also, tumoral vasculatures tend to be leakier vessels leading a trans‐endothelial or transmural fluid flow. However, it not clear whether such elevated flow can control endothelial PD‐L1 expression. Here, new microfluidic device developed investigate the relationship between and microvascular networks (MVNs). After treating MVNs with for 24 h, of cells upregulated. Additionally, CD8 T...
SignificanceAccurate cell segmentation and classification in three-dimensional (3D) images are vital for studying live behavior drug responses 3D tissue culture. Evaluating diverse populations culture over time necessitates non-toxic staining methods, as specific fluorescent tags may not be suitable, immunofluorescence can cytotoxic prolonged cultures.AimWe aim to perform machine learning-based within a heterogeneous population grown relying only on reflectance, transmittance, nuclei...
Immunohistochemistry (IHC) is one of the main clinical techniques for biomarker assessment on tissue biopsies. It consists in chromogenic labeling with specific antibodies, followed by optical imaging, and it used diagnosis therapeutic targeting. A well-known drawback IHC its limited robustness, which often precludes quantitative assessment. We combine microfluidic immunostaining, fluorescence image-based cell segmentation to create an ultrafast procedure accurate via IHC. The experimental...
Immunohistochemistry and fluorescence in situ hybridization are the two standard methods for human epidermal growth factor receptor 2 (HER2) assessment. However, they have severe limitations to assess quantitatively intratumoral heterogeneity (ITH) when multiple subclones of tumor cells co-exist. We develop here a high-content, quantitative analysis breast cancer tissues based on microfluidic experimentation image processing, characterize both HER2 protein overexpression gene amplification...
ABSTRACT Breast cancer desmoplasia heterogeneity contributes to high disease mortality due discrepancies in treatment efficacy between patients. Personalized vitro breast models can be used for throughput testing and ranking of therapeutic strategies normalize the aberrant microenvironment a patient-specific manner. Here, tumoroids assembled from patient-derived cells cultured microphysiological systems including perfusable microvasculature reproduce key aspects stromal vascular dysfunction....