A5024016018- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- NMR spectroscopy and applications
- Pregnancy and preeclampsia studies
- Molecular spectroscopy and chirality
- Computational Drug Discovery Methods
- Reproductive System and Pregnancy
- Food Quality and Safety Studies
- Fungal Biology and Applications
- Spectroscopy and Chemometric Analyses
- Advanced Chemical Sensor Technologies
- Natural Compound Pharmacology Studies
- Natural product bioactivities and synthesis
- Analytical Chemistry and Sensors
- Phytochemistry and Biological Activities
- Ion Transport and Channel Regulation
- Electrolyte and hormonal disorders
- Neurological Disease Mechanisms and Treatments
- Folate and B Vitamins Research
- Maternal and fetal healthcare
- Nutrition, Genetics, and Disease
- Birth, Development, and Health
- Ginkgo biloba and Cashew Applications
- Ginger and Zingiberaceae research
- Tea Polyphenols and Effects
United States Pharmacopeial Convention
2014-2025
Universidad San Pedro
2017
National Institute on Aging
2012
MedStar Health
2010
National Institutes of Health
2005
University of Maryland, Baltimore County
1998
Background Levels of marinobufagenin (MBG), an endogenous bufadienolide Na/K-ATPase (NKA) inhibitor, increase in preeclampsia and NaCl-sensitive hypertension. Methods We tested a 3E9 monoclonal anti-MBG antibody (mAb) for the ability to lower blood pressure (BP) hypertension reverse preeclampsia-induced inhibition erythrocyte NKA. Measurements MBG were performed via immunoassay based on 4G4 mAb. Results In hypertensive Dahl-S rats, intraperitoneal administration 50 μg/kg mAb lowered BP by 32...
Ganoderma lucidum is a well-known medicinal mushroom. At present, numerous G. products have emerged in the form of dietary supplements United States due to its various benefits. However, quality consistency these based on their label ingredients has seldom been evaluated lack suitable toolkit. In this study, 19 batches (Red Reishi, Reishi), herbal/mushroom purchased States, were bioactive components including triterpenes and polysaccharides by using chromatographic methods saccharide...
Objective The pathogenesis of pre-eclampsia (PE), a major cause maternal and fetal mortality, is not fully understood. Digitalis-like sodium pump ligands (SPLs) are believed to be implicated in PE, as illustrated by clinical observations that DIGIBIND, digoxin antibody binds SPLs, lowers blood pressure (BP) PE. We recently reported plasma levels marinobufagenin (MBG), vasoconstrictor SPL, increased four-fold patients with In the present study, we tested whether polyclonal MBG can lower BP...
NMR spectroscopy has recently been utilized to determine the absolute amounts of organic molecules with metrological traceability since signal intensity is directly proportional number each nucleus in a molecule. The methodology that uses hydrogen (1H) quantify chemicals called quantitative 1H-NMR (1H qNMR). method using 1H qNMR for determining purity or content adopted into some compendial guidelines and official standards. However, there are still few reports literature regarding...
Preeclampsia is a major cause of maternal and fetal mortality, its pathogenesis not fully understood. Endogenous digitalis-like cardiotonic steroids (CTS) have been implicated in the pathophysiology preeclampsia; this illustrated by clinical observations that Digibind, therapeutic digoxin antibody fragment which binds CTS, lowers blood pressure reverses Na/K-ATPase inhibition patients with preeclampsia. Recently we reported plasma levels marinobufagenin (MBG), bufadienolide vasoconstrictor...
The ICH guidelines recommend reporting thresholds for regular impurities in drug substances at the level of 0.05% or 0.03% (w/w) depending on maximum daily intake. Therefore, any instrumental method analysis applicable to impurity should be able detect and quantify analytes those levels. This investigation was designed verify suitability 1H NMR spectroscopy detection impurities, as a first step process before attempting quantification. In order minimize demand equipment, this study employed...
Hypericum perforatum L. is the most commonly used herb for treating depression. Due to popularity of this botanical, there a potential economically driven adulteration St. John's wort (SJW) products. The goal study was investigate SJW ingredients suspected be adulterated based on simple preliminary HPTLC tests. Commercial samples were analyzed by following United States Pharmacopeia (USP) monograph methodology, with additional visualization under white light. A number these presented odd...
The present study demonstrates the relationship between conventional and quantum mechanical (QM) NMR spectroscopic analyses, shown here to assist in building a convincingly orthogonal platform for solution documentation of demanding structures. Kaempferol-3-O-robinoside-7-O-glucoside, bisdesmosidic flavonol triglycoside botanical marker aerial parts Withania somnifera, served as an exemplary case. As demonstrated, QM-based 1H iterative full spin analysis (HiFSA) advances understanding both...
Hypericum perforatum L., known as St. John's Wort (SJW), is an important medicinal plant. Aside of being the most commonly used Herbal Medicinal Product for treating depression [1], products made with SJW powdered plant material and various extracts were 9th sold botanical dietary supplement (DS) in mass market channel USA 2012 [2]. Due to popularity this supplement, there a potential economically driven adulteration particularly other species [3].