A5101970038- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Autophagy in Disease and Therapy
- Vitamin D Research Studies
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Exercise and Physiological Responses
- Immune cells in cancer
- Peroxisome Proliferator-Activated Receptors
- Lipid metabolism and biosynthesis
- Sphingolipid Metabolism and Signaling
- Axon Guidance and Neuronal Signaling
- GDF15 and Related Biomarkers
- Muscle Physiology and Disorders
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Pharmacological Effects and Assays
- Receptor Mechanisms and Signaling
- MicroRNA in disease regulation
- Vitamin C and Antioxidants Research
- Phagocytosis and Immune Regulation
- RNA Research and Splicing
- Protease and Inhibitor Mechanisms
Xi'an Jiaotong University
2016-2023
University of Liverpool
2010-2014
Beijing Hospital
2013
Chinese Academy of Medical Sciences & Peking Union Medical College
2013
Adipose tissue expansion during obesity is associated with increased macrophage infiltration. Macrophage-derived factors significantly alter adipocyte function, inducing inflammatory responses and decreasing insulin sensitivity. Identification of the major that mediate detrimental effects macrophages on adipocytes may offer potential therapeutic targets. IL-1β, a proinflammatory cytokine, suggested to be involved in development resistance. This study investigated role IL-1β...
Recent studies have demonstrated brain insulin signaling impairment and mitochondrial dysfunction in diabetes. Hyperinsulinemia hyperlipidemia arising from diabetes been linked to neuronal resistance, hyperglycemia induces peripheral sensory dysfunction. However, how glucose at diabetic conditions elicits cortical remains unknown. In the present study, we cultured primary neurons with high levels investigated function response. We found that was declined presence of 10 mmol/L glucose, prior...
Obesity is associated with a chronic low-grade inflammation and increased macrophage infiltration in adipose tissue. Matrix metalloproteinases (MMPs) are involved tissue remodeling inflammatory responses obesity. This study investigated whether macrophage-derived factors modulate expression secretion of MMP1 MMP3 human preadipocytes. The potential mediators signaling pathways were also explored. primarily expressed secreted by preadipocytes dramatically reduced post-differentiation....
Obesity is associated with skeletal muscle loss and impaired myogenesis. Increased infiltration of proinflammatory macrophages in noted obesity insulin resistance. However, whether the infiltrated can contribute to obesity‐induced unclear. In this study, we investigate macrophage differentiation markers quadriceps (QC), gastrocnemius, tibia anterior, soleus muscles from obese mice that were fed a high‐fat diet for 16 weeks. Then, examined effect mediator macrophage‐secreted factors on...
Previous studies by Tisdale et al. have reported that zinc-α(2)-glycoprotein (ZAG (AZGP1)) reduces body fat content and improves glucose homeostasis the plasma lipid profile in Aston (ob/ob) mice. It has been suggested this might be mediated via agonism of β(3)- possibly β(2)-adrenoceptors. We compared effects dosing recombinant human ZAG (100 μg, i.v.) BRL35135 (0.5 mg/kg, i.p.), which is rodents a 20-fold selective relative to β(2)-adrenoceptor agonist, given once daily for 10 days male...
Oxysterols are critical regulators of inflammation and cholesterol metabolism in cells. They oxidation products may be differentially metabolised subcellular compartments biological fluids. New analytical methods needed to improve our understanding oxysterol trafficking the molecular interplay between cellular required maintain cholesterol/oxysterol homeostasis. Here we describe a method for isolation oxysterols using solid phase extraction quantification by liquid chromatography-mass...
Aims: To study the expression and regulatory role of SOD3 in adipocytes adipose tissue. Results: was determined various tissues adult C57BL/6J mice, human tissue epididymal tissue, subcutaneous brown high-fat diet (HFD)-induced obese mice. release were evaluated differentiated from primary preadipocytes murine bone marrow-derived mesenchymal stem cells (BM-MSCs). The for by lentivirus knockdown global sod3 knockout (KO) expressed at high levels white main expressing significantly elevated...
Growing evidence has suggested that autophagy-related protein 7 (ATG7) plays an important role in insulin signaling, but the mechanism of ATG7 hepatic sensitivity is not fully understood. The purpose present study to clarify underlying molecular mechanisms obesity development. Serum and liver samples from mice fed a high fat diet (HFD) were evaluated for metabolic profile data ATG expressions during We found compared with other ATGs, expression increased earlier lower 4-wk HFD-fed mice. For...
目的 探讨硫化氢（H2S）对Aβ25-35诱导的小鼠成神经细胞瘤细胞Neuro-2a自噬的影响及其潜在机制。 方法 将Neuro-2a细胞随机分为空白对照组、Aβ25-35处理组、Aβ25-35+NaHS组、Aβ25-35+3-甲基腺嘌呤（3-MA）组、NaHS组及Aβ25-35+NaHS+LY294002组。Aβ25-35+NaHS组和Aβ25-35+3-MA组细胞分别用NaHS和3-MA预处理2 h后均加用Aβ25-35继续处理24 h；Aβ25-35+NaHS+LY294002组除加入NaHS预处理外需在Aβ25-35处理前0.5 h加入特异性PI3K/Akt通路抑制剂LY294002。采用MTT法观察各组细胞存活率，蛋白质印迹法检测自噬蛋白Beclin-1、微管相关蛋白（LC3）及P62的表达，免疫荧光法检测LC3的表达及分布，透射电镜法观察自噬体的形成。 结果 （1）与空白对照组相比，Aβ25-35作用细胞后细胞存活率降低（P < 0.05），Beclin-1及LC3-Ⅱ表达增加，P62表达降低（P...