A5100443125- Renal Transplantation Outcomes and Treatments
- Retinoids in leukemia and cellular processes
- Acute Myeloid Leukemia Research
- Pharmacological Effects and Toxicity Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Hepatitis C virus research
- HIV/AIDS drug development and treatment
- Advanced biosensing and bioanalysis techniques
- Gut microbiota and health
- Cancer, Lipids, and Metabolism
- Liver Disease Diagnosis and Treatment
- Adipose Tissue and Metabolism
- Glycosylation and Glycoproteins Research
- Hepatitis B Virus Studies
- Cancer therapeutics and mechanisms
- Carbohydrate Chemistry and Synthesis
- Organ and Tissue Transplantation Research
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Sepsis Diagnosis and Treatment
- Protein Degradation and Inhibitors
- Lymphoma Diagnosis and Treatment
- Liver Disease and Transplantation
Second Hospital of Tianjin Medical University
2018-2025
Tianjin Medical University
2018-2025
Xichang University
2025
Wenzhou City People's Hospital
2024
Albert Einstein College of Medicine
2010-2024
United Nations Economic and Social Commission for Asia and the Pacific
2021-2024
Weifang People's Hospital
2023
Northwest University
2023
China National Center for Food Safety Risk Assessment
2023
Guangdong Academy of Agricultural Sciences
2023
Abstract Background More extensive use of metagenomic shotgun sequencing in microbiome research relies on the development high-throughput, cost-effective sequencing. Here we present a comprehensive evaluation performance new high-throughput platform BGISEQ-500 for and compare its with that 2 Illumina platforms. Findings Using fecal samples from 20 healthy individuals, evaluated intra-platform reproducibility setup comprising 8 library replicates replicates. Cross-platform consistency was by...
Association mapping can quickly and efficiently dissect complex agronomic traits. Rapeseed is one of the most economically important polyploid oil crops, although its genome sequence not yet published. In this study, a recently developed 60K Brassica Infinium(®) SNP array was used to analyse an association panel with 472 accessions. The single-nucleotide polymorphisms (SNPs) were in silico mapped using 'pseudomolecules' representative rapeseed establish their hypothetical order perform seed...
ABSTRACT Specialized transduction has proven to be useful for generating deletion mutants in most mycobacteria, including virulent Mycobacterium tuberculosis . We have improved this system by developing (i) a single-step strategy the construction of allelic exchange substrates (AES), (ii) temperature-sensitive shuttle phasmid with greater cloning capacity than phAE87, and (iii) bacteriophage-mediated transient expression site-specific recombinase precisely excise antibiotic markers. The...
Adipose tissue remodeling occurs in obesity, characterized by adipocyte hypertrophy and increased infiltration of macrophages which also shift to a proinflammatory phenotype. Factors derived from these significantly alter function, such as repressing adipogenesis, inducing inflammatory response desensitizing insulin action. As produce cocktail signals, identifying the key factors that mediate detrimental effects may offer effective therapeutic targets. IL-1β, major cytokine produced largely...
Increased accumulation of macrophages in adipose tissue obesity is linked to low-grade chronic inflammation, and associated with features metabolic syndrome. Vitamin D3 may have immunoregulatory effects reduce although the molecular mechanisms remain be established. This study investigated vitamin on macrophage-elicited inflammatory responses cultured human adipocytes, particularly signalling pathways involved. Macrophage-conditioned (MC) medium (25% adipocyte maintenance media) markedly...
Abstract Circular RNAs (circRNAs) are a novel class of powerful regulators in gene expression and participate the pathogenesis many diseases, including cancer. However, little is known about roles circRNAs development treatment acute promyelocytic leukemia (APL). Here we report profiling function APL, their dynamic regulation during all- trans retinoic acid (ATRA)-induced differentiation. We performed two independent ribosomal RNA-minus RNA-sequencing (Ribo-minus RNA-seq) experiments with...
The human tubercle bacillus Mycobacterium tuberculosis can synthesize NAD(+) using the de novo biosynthesis pathway or salvage pathway. of bovine bovis was reported defective due to a mutation in nicotinamidase PncA. This defect prevents nicotinic acid secretion, which is basis for niacin test that clinically distinguishes M. from tuberculosis. Surprisingly, we found NAD(+)de (nadABC) be deleted bovis, demonstrating functioning bovisDeltanadABC fails grow mice, whereas...
Purpose: The ganglioside fucosyl-GM1 (FucGM1) is a tumor-associated antigen expressed in large percentage of human small cell lung cancer (SCLC) tumors, but absent most normal adult tissues, making it promising target immuno-oncology. This study was undertaken to evaluate the preclinical efficacy BMS-986012, novel, nonfucosylated, fully IgG1 antibody that binds specifically FucGM1.Experimental Design: antitumor activity BMS-986012 evaluated vitro assays using SCLC cells and mouse xenograft...
The standard treatment for Glioblastoma multiforme (GBM) is surgical resection and subsequent radiotherapy chemotherapy. Surgical of GBM typically restricted because its invasive growth, which results in residual tumor cells including glioma stem (GSCs) differentiated cells. Recurrence has been previously thought to occur as a result these GSCs, hypoxic microenvironment maintains the GSCs stemness also plays an important role. Summarizing traditional studies we find many researchers ignored...
1. The global diversity of domestic chicken breeds, each exhibiting unique and specialised traits, offers a compelling context to explore how selection influences genetic variation patterns. China, with its myriad local contributes significantly this diversity.
Sepsis is a life-threatening syndrome caused by an imbalance in the inflammatory response to infection that can lead high mortality rate. Escherichia coli common pathogen causes sepsis. The role of immunoglobulin G N-glycome estimating patients with sepsis remains unknown. This study aims reveal clinical application as potentially novel biomarker predict risk coli-induced serum levels 100 adult septic patient samples on day intensive care unit (ICU) admission, and healthy volunteers were...
Obesity is associated with a chronic low-grade inflammation and increased macrophage infiltration in adipose tissue. Matrix metalloproteinases (MMPs) are involved tissue remodeling inflammatory responses obesity. This study investigated whether macrophage-derived factors modulate expression secretion of MMP1 MMP3 human preadipocytes. The potential mediators signaling pathways were also explored. primarily expressed secreted by preadipocytes dramatically reduced post-differentiation....
The Arabidopsis genome encodes one potential isopropylmalate isomerase (IPMI) large subunit and three IPMI small subunits, which in bacteria archaea form heterodimers to catalyze the isomerization of 2-isopropylmalate 3-isopropylmalate leucine biosynthesis. We demonstrate here that AtLeuC physically interacts with AtLeuD proteins functional IPMIs. IPMIs are localized chloroplast stroma. Tissue-specific expression analysis revealed patterns AtLeuD1 AtLeuD2 similar, but distinct from AtLeuD3....
Adipose tissue has been identified as an endocrine organ secreting adipokines involved in metabolic and inflammatory pathways. Adiponectin, anti-inflammatory adipokine, is reduced sepsis. High Molecular Weight (HMW) adiponectin, the biologically most relevant molecule, investigated very little human Zinc-alpha2-glycoprotein (ZAG) a novel adipokine its expression adipose positively correlated with adiponectin expression. It not yet known whether ZAG role In this study we assessed levels of...
Over 10 million people developed tuberculosis (TB) in 2016, and over 1.8 individuals succumbed to the disease. These numbers make TB ninth leading cause of death worldwide from a single infectious agent. Therefore, finding novel therapeutic targets Mycobacterium , pathogen that causes most cases human TB, is critical. In this study, we reveal virulence factor M. nrp gene. The lack highly attenuates course infection mouse model, which particularly relevant immune-deficient hosts. This very as...
The objective of this study is to investigate the correlation between methods high-performance liquid chromatography (HPLC) and enzyme multiplied immunoassay technology (EMIT) for determination total mycophenolic acid (tMPA) free (fMPA) concentration pharmacokinetics fMPA in Chinese liver transplant recipients. An HPLC method with fluorometric detection an EMIT assay were established determine plasma ultrafiltrates. Pharmacokinetic parameters tMPA 51 patients estimated. calibration range was...
Summary Aims Acute cerebral ischemia may lead to ischemic stroke, which is a major cause of death and disability worldwide. Hydrogen sulfide (H 2 S) functions importantly in mammalian systems. The present work was designed study the effect sodium sulfide, donor H S, on acute ischemia. Methods focal produced by middle artery occlusion ( MCAO ) Sprague‐Dawley SD rats. Bilateral vertebral arteries common carotid were blocked establish global anoxia hypobaric C57BL/6 mice hypoxic Nimodipine...