Prafulla Bhagat
A5014226372- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Multiple Myeloma Research and Treatments
- Chronic Myeloid Leukemia Treatments
- RNA Interference and Gene Delivery
- Platelet Disorders and Treatments
- Cancer-related gene regulation
- Click Chemistry and Applications
- Acute Lymphoblastic Leukemia research
- Chronic Lymphocytic Leukemia Research
- Pancreatic and Hepatic Oncology Research
- Protein Degradation and Inhibitors
- Advanced Combustion Engine Technologies
- Biodiesel Production and Applications
- Histone Deacetylase Inhibitors Research
- Hybrid Renewable Energy Systems
- Virus-based gene therapy research
Albert Einstein College of Medicine
2017-2022
Montefiore Medical Center
2017-2022
Montefiore Einstein Comprehensive Cancer Center
2022
Even though pancreatic ductal adenocarcinoma (PDAC) is associated with fibrotic stroma, the molecular pathways regulating formation of cancer fibroblasts (CAFs) are not well elucidated. An epigenomic analysis patient-derived and de-novo generated CAFs demonstrated widespread loss cytosine methylation that was overexpression various inflammatory transcripts including CXCR4. Co-culture neoplastic cells led to increased invasiveness abrogated by inhibition Metabolite tracing revealed lactate...
Abstract The bone marrow microenvironment influences malignant hematopoiesis, but how it promotes leukemogenesis has not been elucidated. In addition, the role of stroma in regulating clinical responses to DNA methyltransferase inhibitors (DNMTi) is also poorly understood. this study, we conducted a methylome analysis marrow–derived stromal cells from myelodysplastic syndrome (MDS) patients and observed widespread aberrant cytosine hypermethylation occurring preferentially outside CpG...
Abstract Purpose: Thrombocytopenia is a serious complication of myelodysplastic syndromes (MDS) associated with an increased bleeding risk and worse prognosis. Eltrombopag (ELT), thrombopoietin receptor agonist, can increase platelet counts reverse anti-megakaryopoietic effects lenalidomide (LEN) in preclinical studies. We hypothesized ELT would reduce the incidence thrombocytopenia MDS. Patients Methods: conducted Phase II multicenter trial LEN adult patients low- or intermediate-1–risk MDS...
<p>Supplementary Methods</p>
<p>Supp Fig 1 : SFRP1 methylation with coculture Supp 2 Validation of changes stroma and leukemic cell 3: FRZB upregulation in MDS after 5-Azacytidine treatment SuppFig 4: knockdown leads to partial inhibition erythroid differentiation induced by stromawith 5-Aza 5: The expression WNT signature genes control marrow derived CD34+ cells</p>
<p>Supp Table 1: MDS Patient Disease Characteristics Supp 2: Pathways affected by hypermethylation in stroma 3: Genes that are methylated and underexpressed 4. patient characteristics for primary MSC sample isolation table 5: WNT Targets</p>
<div>Abstract<p>The bone marrow microenvironment influences malignant hematopoiesis, but how it promotes leukemogenesis has not been elucidated. In addition, the role of stroma in regulating clinical responses to DNA methyltransferase inhibitors (DNMTi) is also poorly understood. this study, we conducted a methylome analysis marrow–derived stromal cells from myelodysplastic syndrome (MDS) patients and observed widespread aberrant cytosine hypermethylation occurring preferentially...
<p>Supplementary Methods</p>
<p>Supp Fig 1 : SFRP1 methylation with coculture Supp 2 Validation of changes stroma and leukemic cell 3: FRZB upregulation in MDS after 5-Azacytidine treatment SuppFig 4: knockdown leads to partial inhibition erythroid differentiation induced by stromawith 5-Aza 5: The expression WNT signature genes control marrow derived CD34+ cells</p>
<p>Supp Table 1: MDS Patient Disease Characteristics Supp 2: Pathways affected by hypermethylation in stroma 3: Genes that are methylated and underexpressed 4. patient characteristics for primary MSC sample isolation table 5: WNT Targets</p>
<p>Supplemental Figure 1. Overall Survival Responders vs Non-Responders Supplemental 2. Among Groups</p>
<div>AbstractPurpose:<p>Thrombocytopenia is a serious complication of myelodysplastic syndromes (MDS) associated with an increased bleeding risk and worse prognosis. Eltrombopag (ELT), thrombopoietin receptor agonist, can increase platelet counts reverse anti-megakaryopoietic effects lenalidomide (LEN) in preclinical studies. We hypothesized ELT would reduce the incidence thrombocytopenia MDS.</p>Patients Methods:<p>We conducted Phase II multicenter trial LEN adult...
<div>AbstractPurpose:<p>Thrombocytopenia is a serious complication of myelodysplastic syndromes (MDS) associated with an increased bleeding risk and worse prognosis. Eltrombopag (ELT), thrombopoietin receptor agonist, can increase platelet counts reverse anti-megakaryopoietic effects lenalidomide (LEN) in preclinical studies. We hypothesized ELT would reduce the incidence thrombocytopenia MDS.</p>Patients Methods:<p>We conducted Phase II multicenter trial LEN adult...
<p>Supplemental Figure 1. Overall Survival Responders vs Non-Responders Supplemental 2. Among Groups</p>
<div>Abstract<p>The bone marrow microenvironment influences malignant hematopoiesis, but how it promotes leukemogenesis has not been elucidated. In addition, the role of stroma in regulating clinical responses to DNA methyltransferase inhibitors (DNMTi) is also poorly understood. this study, we conducted a methylome analysis marrow–derived stromal cells from myelodysplastic syndrome (MDS) patients and observed widespread aberrant cytosine hypermethylation occurring preferentially...