A5107970501- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Toxin Mechanisms and Immunotoxins
- Vector-borne infectious diseases
- Signaling Pathways in Disease
- Plant pathogens and resistance mechanisms
- Biochemical and Molecular Research
- Parasites and Host Interactions
- Insect and Pesticide Research
- Calcium signaling and nucleotide metabolism
- Zoonotic diseases and public health
- Caveolin-1 and cellular processes
- Ion channel regulation and function
- Viral Infections and Outbreaks Research
- Extracellular vesicles in disease
- Bacteriophages and microbial interactions
- Insect Pest Control Strategies
- Synthesis and Biological Evaluation
- Surgical Sutures and Adhesives
- Parkinson's Disease Mechanisms and Treatments
- Reconstructive Surgery and Microvascular Techniques
- Cancer Mechanisms and Therapy
- Galectins and Cancer Biology
- Cancer, Stress, Anesthesia, and Immune Response
- Viral Infectious Diseases and Gene Expression in Insects
Universidade de São Paulo
1996-2024
Institute of Biomedical Science
2020-2024
Universidade Cidade de São Paulo
2020
University of Maryland, College Park
2011-2013
Yale University
2011
Universidade Federal de São Paulo
2002-2009
University of Antofagasta
2008
Hospital São Paulo
1996
Rapid repair of plasma membrane wounds is critical for cellular survival. Muscle fibers are particularly susceptible to injury, and defective sarcolemma resealing causes muscular dystrophy. Caveolae accumulate in dystrophic muscle caveolin cavin mutations cause pathology, but the underlying mechanism unknown. Here we show that other cell types by a involving Ca2+-triggered exocytosis lysosomes, release acid sphingomyelinase, rapid lesion removal caveolar endocytosis. Wounding or exposure...
Upon host cell contact, the protozoan parasite Trypanosoma cruzi triggers cytosolic Ca2+ transients that induce exocytosis of lysosomes, a process required for invasion. However, exact mechanism by which lysosomal mediates T. internalization remains unclear. We show entry mimics plasma membrane injury and repair involves Ca2+-dependent delivery acid sphingomyelinase (ASM) to outer leaflet membrane, rapid form endocytosis internalizes lesions. Host cells incubated with trypomastigotes are...
During its life cycle, Leishmania undergoes extreme environmental changes, alternating between insect vectors and vertebrate hosts. Elevated temperature decreased pH, conditions encountered after macrophage invasion, can induce axenic differentiation of avirulent promastigotes into virulent amastigotes. Here we show that iron uptake is a major trigger for the amazonensis amastigotes, independently pH changes. We found depletion from culture medium triggered expression ferrous transporter...
ABSTRACT Upon oral infection, Trypanosoma cruzi metacyclic trypomastigotes invade and replicate in the gastric mucosal epithelium, being apparently uniquely specialized for adhesion to mucin invasion. Here we investigated involvement of gp82, metacyclic-stage-specific surface glycoprotein implicated host cell entry, both invasion epithelium upon challenge. Metacyclic forms, preincubated with a control monoclonal antibody (MAb) or MAb 3F6 directed were administered orally BALB/c mice,...
The disassembly of host cell actin cytoskeleton as a facilitator Trypanosoma cruzi invasion has been reported by some authors, while other workers claim that it instead inhibits internalization the parasite. In this study we aimed at elucidating basis discrepancy. We performed experiments with metacyclic trypomastigotes T. strains G and CL, which differ markedly in infectivity enter target cells engaging surface molecules gp35/50 gp82, respectively, have signaling activity. Treatment HeLa...
Trypanosoma cruzi, the etiological agent of Chagas disease, consumes glucose and amino acids depending on environmental availability each nutrient during its complex life cycle. For example, are major energy carbon sources in intracellular stages T. cruzi parasite, but their consumption produces an accumulation NH4+ environment, which is toxic. These parasites do not have a functional urea cycle to secrete excess nitrogen as low-toxicity waste. Glutamine synthetase (GS) plays central role...
Virulent protozoans named Leishmania in tropical and subtropical areas produce devastating diseases by exploiting host immune responses. Amastigotes of amazonensis stimulate macrophages to express CD200, an immunomodulatory ligand, which binds its cognate receptor (CD200R) inhibits the inducible nitric oxide synthase (iNOS/NO) signaling pathways, thereby promoting intracellular survival. However, mechanisms underlying CD200 induction remain largely unknown. Here, we show that...
Triple-negative breast cancer (TNBC) is an aggressive tumor with limited treatment options and poor prognosis. We applied the in vivo phage display technology to isolate peptides homing immunosuppressive cellular microenvironment of TNBC as a strategy for non-malignant target discovery. identified cyclic peptide (CSSTRESAC) that specifically binds vitamin D receptor, protein disulfide-isomerase A3 (PDIA3) expressed on cell surface tumor-associated macrophages (TAM), targets syngeneic TNBC,...
Trypanosoma cruzi infection is known to confer resistance tumor development in mice, and in-vitro studies have shown the toxic effects of parasite extracts on cancer cell cultures. Investigations which T. molecules exhibit antitumor activity just begun. Here, we used a tumorigenic line Tm5, derived from mouse melanocytes melan-a, test effect J18, recombinant protein based surface molecule gp82 fused glutathione-S-transferase (GST). J18 induced actin cytoskeleton disruption Tm5 but not...
Abstract Cutaneous leishmaniasis (CL) is a neglected disease that promotes destructive lesions. Difficulties in treatment are related to accessibility of drugs, resistance and toxicity. Antimicrobial photodynamic therapy (APDT) has been emerging as promising for CL. In this work, we evaluated methylene blue (MB)‐mediated APDT (MB‐APDT) on Leishmania amazonensis vitro vivo by bioluminescence technique. , MB‐APDT was performed using red LED (λ = 660 ± 11 nm, 100 mW cm −2 ) MB (100 µ m at...
Pseudomonas aeruginosa is a multidrug-resistant opportunistic pathogen associated with life-threatening nosocomial and community-acquired infections. Antibiotic resistance an immediate threat to public health demands urgent action discovering new antimicrobial agents. One of the best alternatives for pre-clinical tests animal models greater wax moth Galleria mellonella. Here, we evaluated antipseudomonal activity silver nanoparticles (AgNPs) using G. mellonella larvae. The AgNPs were...
The authors' experience of 51 posterior interosseous forearm island flaps is presented. Its main indications were inadequate scar or contraction and complex trauma with gross skin loss, either acute postprimary. flap healed uneventfully in 45 cases (88%), but six it was partially subtotally lost due to necrosis. Late complications included hair growth the first web palm, redundancy hypertrophic at donor site. produces excellent coverage difficult raise tends develop oedema occasionally
ABSTRACT Trypanosoma cruzi metacyclic trypomastigotes invade and replicate in the gastric mucosal epithelium after oral infection. In this study we analyzed process of infection by T. isolates deficient expression gp82, stage-specific surface glycoprotein implicated target cell entry vitro promoting mice challenge. Mice infected route with forms gp82-deficient isolate 569 or 588 developed patent parasitemia but at greatly reduced levels compared to those gp82-expressing CL. Metacyclic both...
Parasitological cure for Chagas disease is considered extremely difficult to achieve because of the lack effective chemotherapeutic agents against Trypanosoma cruzi at different stages infection. There are currently only two drugs available. These have several limitations and can produce serious side effects. Thus, new targets much sought after. Among T. components involved in key processes such as parasite proliferation host cell invasion, Ca2+-dependent molecules play an important role....