A5047820723- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- Wound Healing and Treatments
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Genomics and Chromatin Dynamics
- Cancer Mechanisms and Therapy
- Cell death mechanisms and regulation
- Effects of Radiation Exposure
- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- 3D Printing in Biomedical Research
- Epigenetics and DNA Methylation
- Signaling Pathways in Disease
- Cytokine Signaling Pathways and Interactions
- Chemokine receptors and signaling
- Histone Deacetylase Inhibitors Research
- Angiogenesis and VEGF in Cancer
- Cellular Mechanics and Interactions
- Mechanisms of cancer metastasis
- Protist diversity and phylogeny
- Sirtuins and Resveratrol in Medicine
Baylor College of Medicine
2022-2025
Houston Methodist
2014-2022
Cornell University
2018
Methodist Hospital
2018
University of California, San Francisco
2006-2016
Lawrence Berkeley National Laboratory
2011-2014
University of California, Berkeley
2014
Purdue University West Lafayette
2006-2011
University of California, Irvine
2009
University of Chicago
2008
The molecular mechanisms governing acquired tumor resistance during radiotherapy remain to be elucidated. In breast cancer patients, overexpression of HER2 (human epidermal growth factor receptor 2) is correlated with aggressive and increased recurrence. the present study, we demonstrate that expression can induced by radiation in cells a low basal level HER2. Furthermore, HER2-postive tumors occur at much higher frequency recurrent invasive (59%) compared primary (41%). Interestingly,...
c-MYC promotes cisplatin resistance by enabling the increased activity of a DNA repair enzyme.
Abstract β1-integrin induction enhances breast cancer cell survival after exposure to ionizing radiation (IR), but the mechanisms of this effect remain unclear. Although NF-κB initiates prosurvival signaling pathways post-IR, molecular function with other key elements in radioresistance, particularly respect extracellular matrix-induced signaling, is not known. We discovered a typical NF-κB–binding site promoter region, indicating possible regulatory role for NF-κB. Using three-dimensional...
Microhomology-mediated end joining (MMEJ), an error-prone pathway for DNA double-strand break (DSB) repair, is implicated in genomic rearrangement and oncogenic transformation; however, its contribution to repair of radiation-induced DSBs has not been characterized. We used recircularization a linearized plasmid with 3΄-P-blocked termini, mimicking those at X-ray-induced strand breaks, recapitulate DSB via MMEJ or nonhomologous end-joining (NHEJ). Sequence analysis the circularized plasmids...
Background The existence of immunologically ‘cold tumors’ frequently found across a wide spectrum tumor types represents significant challenge for cancer immunotherapy. Cold tumors have poor baseline pan-leukocyte infiltration, including low prevalence cytotoxic lymphocytes, and not surprisingly respond unfavorably to immune checkpoint (IC) inhibitors. We hypothesized that cold harbor mechanism escape upstream independent ICs may be driven by biology rather than differences in mutational...
Abstract Mechanisms governing inducible resistance to ionizing radiation in untransformed epithelial cells pre-exposed low-dose (LDIR; ≤10 cGy) are not well understood. The present study provides evidence that pre-exposure 10 cGy X-rays increases clonogenic survival of mouse skin JB6P+ subsequently exposed 2 Gy doses γ-rays. To elucidate the molecular pathways LDIR-induced adaptive radioresistance, transcription factor nuclear factor-κB (NF-κB) and a group NF-κB–related proteins [i.e., p65,...
The chromatin remodeling factor SMARCAD1, an SWI/SNF ATPase family member, has a role in 5′ end resection at DNA double-strand breaks (DSBs) to produce single-strand (ssDNA), critical step for subsequent checkpoint and repair loading remove damage. However, the mechanistic details of SMARCAD1 coupling damage response pathways remains unknown. Here we report that is recruited DSBs through ATM-dependent process. Depletion reduces ionizing radiation (IR)-induced repairosome foci formation DSB...
For patients with head and neck squamous cell carcinoma (HNSCC), failure of definitive radiation combined cisplatin nearly universally results in death. Although hyperactivation the Nrf2 pathway can drive resistance along suppressed anti-tumor immunity, treatment-refractory HNSCC tumors may retain sensitivity to targeted agents secondary synergistic lethality other oncogenic drivers (e.g., NOTCH1 mutations). We evaluated efficacy PI3K inhibitors (PI3Ki) bypassing Nrf2-mediated HNSCC....
The molecular mechanism by which tumor cells increase their resistance to therapeutic radiation remains be elucidated. We have previously reported that activation of nuclear factor-kappaB (NF-kappaB) is causally associated with the enhanced cell survival MCF+FIR derived from breast cancer MCF-7 after chronic exposure fractionated ionizing radiation. aim present study was reveal context NF-kappaB pathways in adaptive radioresistance. Using lines isolated populations, we found elevated...
The emergence of Vibrio cholerae O139 Bengal in 1993, its rapid spread an epidemic form, which it replaced existing strains V. O1 during 1992 and the subsequent reemergence El Tor biotype Bangladesh since 1994 have raised questions regarding origin reemerged vibrios. We studied 50 vibrio isolated four other countries Asia Africa before 32 after caused by to determine whether vibrios were genetically different from existed O139. Analysis restriction fragment length polymorphisms genes for...
Abstract Introduction Ductal carcinoma in situ (DCIS) is characterized by non-invasive cancerous cell growth within the breast ducts. Although radiotherapy commonly used treatment of DCIS, effect and molecular mechanism ionizing radiation (IR) on DCIS are not well understood, invasive recurrence following remains a significant clinical problem. This study investigated effects IR clinically relevant model Akt-driven identified possible mechanisms underlying progression surviving cells....
The molecular mechanisms underlying resistance to radiotherapy in breast cancer cells remain elusive. Previously, we reported that elevated β1-integrin is associated with enhanced cell survival postirradiation, but how conferred radioresistance was unclear. Ionizing radiation (IR) induced killing correlates the efficiency of DNA double-strand break (DSB) repair, and found nonmalignant epithelial (S1) low expression have a higher frequency S-phase-specific IR-induced chromosomal aberrations...
Vibrio cholerae O139 Bengal emerged in 1992 and rapidly spread an epidemic form, which it replaced existing strains of V. O1 Bangladesh during 1993. The subsequent emergence a new clone the El Tor biotype that transiently displaced vibrios 1994 to 1995 recent reemergence its coexistence with demonstrated temporal changes epidemiology cholera Bangladesh. We studied clonal diversity among isolated from patients environmental surface water since their first appearance until transient...
The high mobility group AT-hook 2 (HMGA2), a DNA architectural protein, is highly regulated during development and plays an important role in tumorigenesis. Indeed, HMGA2 was overexpressed many different kinds of tumors. However, the mechanisms regulating expression remain elusive. Using microarray analysis, we found that HMGA2, along with dozen other genes, co-repressed by ZBRK1, BRCA1, CtIP. BRCA1 exerts its transcriptional repression activity through interaction repressor ZBRK1 central...
The human MOF (hMOF) protein belongs to the MYST family of histone acetyltransferases and plays a critical role in transcription DNA damage response. is essential for cell proliferation; however, its during replication replicative stress unknown. Here we demonstrate that cells depleted under induced by cisplatin, hydroxyurea, or camptothecin have reduced survival, higher frequency S-phase-specific chromosome damage, increased R-loop formation. depletion decreased fork speed and, when...
Hyperthermia inhibits DNA double-strand break (DSB) repair that utilizes homologous recombination (HR) pathway by a poorly defined mechanism(s); however, the mechanisms for this inhibition remain unclear. Here we report hyperthermia decreases H4K16 acetylation (H4K16ac), an epigenetic modification essential genome stability and transcription. Heat-induced reduction in H4K16ac was detected humans,
Pulmonary fibrosis (PF) is a major side effect of radiotherapy and chemotherapy. Recent clinical trials, unfortunately, have failed to identify any therapeutic agent which has the potential reduce consequences this devastating condition. Reactive oxygen species tissue remodeling regulators, such as metalloproteinases (MMPs) their inhibitors (TIMPs), are thought be involved in development PF. We investigated these factors determine protective effects antioxidant alpha-lipoic acid (LA) against...