A5014866366- Prostate Cancer Treatment and Research
- Cancer, Lipids, and Metabolism
- Head and Neck Cancer Studies
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Prostate Cancer Diagnosis and Treatment
- Bladder and Urothelial Cancer Treatments
- Cancer-related gene regulation
- Molecular Biology Techniques and Applications
- Esophageal Cancer Research and Treatment
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Radiomics and Machine Learning in Medical Imaging
- Renal cell carcinoma treatment
- Urinary Bladder and Prostate Research
- CAR-T cell therapy research
- Immunodeficiency and Autoimmune Disorders
- Cancer-related Molecular Pathways
- Fibroblast Growth Factor Research
- Hepatitis B Virus Studies
Baylor College of Medicine
2015-2025
Michael E. DeBakey VA Medical Center
2009-2023
Dan L Duncan Comprehensive Cancer Center
2020-2023
Hospital Universitario Infanta Cristina
2015-2021
United States Department of Veterans Affairs
2014
SUNY Downstate Health Sciences University
2011
State University of New York
2011
The University of Texas MD Anderson Cancer Center
1998-2005
Veterans Health Administration
2004
No single cancer immunotherapy will likely defeat all evasion mechanisms of solid tumors, including plasticity tumor antigen expression and active immune suppression by the environment. In this study, we increase breadth, potency, duration anti-tumor activity chimeric receptor (CAR) T cells using an oncolytic virus (OV) that produces cytokine, checkpoint blockade, a bispecific tumor-targeted cell engager (BiTE) molecule. First, constructed BiTE molecule specific for CD44 variant 6 (CD44v6),...
Bulk and single-cell RNA sequencing do not provide full characterization of tissue spatial diversity in cancer samples, currently available
Abstract BACKGROUND Senescent cells accumulate in tissues with age and show changes protein expression that may influence the function of adjacent contribute to development tissue pathologies associated aging. Benign prostatic hyperplasia (BPH) is an extremely common disease older men characterized by increased growth epithelial stromal cells. In BPH, there Il‐1α results elevated FGF7 cells, which turn strongly correlated proliferation. METHODS Human BPH primary cultures were analyzed...
BACKGROUND Benign prostatic hyperplasia (BPH) is characterized by increased tissue mass in the transition zone of prostate, which leads to obstruction urine outflow and significant morbidity majority older men. Plasma markers oxidative stress are men with BPH but it unclear whether and/or DNA damage causal pathogenesis BPH. METHODS Levels 8‐OH deoxyguanosine (8‐OH dG), a marker stress, were measured prostate tissues from normal ELISA. dG was also detected immunohistochemistry staining...
// Michael Brooks 1, * , Qianxing Mo 2, 6, Ross Krasnow 1 Philip Levy Ho 3 Yu-Cheng Lee 4 Jing Xiao Antonina Kurtova Seth Lerner Gui Godoy Weiguo Jian Patricia Castro 5, 6 Fengju Chen 2 David Rowley 4, Ittmann Keith Syson Chan 3, 7, 8 Scott Department of Urology, Baylor College Medicine, One Plaza, Houston, Texas, 77030 Kelsey-Seybold Clinic, Texas Molecular & Cellular Biology, 5 Pathology and Immunology, E. DeBakey VAMC, Dan L Duncan Cancer Center, 7 Center for Cell, Gene Therapy, Drug...
Abstract Background Human papillomavirus (HPV)–associated oropharyngeal squamous cell carcinoma (OPSCC) has excellent control rates compared to nonvirally associated OPSCC. Multiple trials are actively testing whether de-escalation of treatment intensity for these patients can maintain oncologic equipoise while reducing treatment-related toxicity. We have developed OP-TIL, a biomarker that characterizes the spatial interplay between tumor-infiltrating lymphocytes (TILs) and surrounding cells...
African-American (AA) men are more likely to be diagnosed with and die from prostate cancer than European American (EA) men. Despite the central role of androgen receptor (AR) transcription factor in cancer, little is known about contribution epigenetics observed racial disparities. We performed AR chromatin immunoprecipitation sequencing on primary tumors AA EA men, finding that sites greater binding intensity relative enriched for lipid metabolism immune response genes. Integration...
Abstract A considerable body of evidence indicates that alterations fibroblast growth factors (FGFs) and their receptors contribute to prostate cancer progression. Recently, a new family regulators FGF activity has been identified. The Sprouty gene negatively regulates signaling in variety systems could potentially limit the biological FGFs cancer. Immunohistochemical analysis normal neoplastic tissues using tissue microarrays revealed Sprouty1 protein is down-regulated approximately 40%...
Abstract BACKGROUND Benign prostatic hyperplasia (BPH) is an extremely common disease of older men characterized by increased growth epithelial and stromal cells. Previously we showed that senescent cells accumulate in the prostate aging secrete interleukin‐1α (IL‐1α). IL‐8 also present at levels BPH tissues induces expression FGF2, a potent factor. Therefore, sought to determine if expressed this secreted plays role pathogenesis BPH. METHODS Expression human tissue primary cultures was...
Abstract Incidence and mortality rates for prostate cancer are higher in African-American (AA) men than European-American (EA) men, but the biologic basis this disparity is unclear. We carried out a detailed analysis of gene expression changes compared with their matched benign tissues cohort AA them existing data from EA men. In manner, we identified MNX1 as novel oncogene upregulated to relatively greater degree Androgen AKT signaling play central role pathogenesis found that both these...
BACKGROUND. Patients with p16+ oropharyngeal squamous cell carcinoma (OPSCC) are potentially cured definitive treatment. However, there currently no reliable biomarkers of treatment failure for OPSCC. Pathologist-based visual assessment tumor multinucleation (MN) has been shown to be independently prognostic disease-free survival (DFS) in its quantification is time intensive, subjective, and at risk interobserver variability.
For patients with head and neck squamous cell carcinoma (HNSCC), failure of definitive radiation combined cisplatin nearly universally results in death. Although hyperactivation the Nrf2 pathway can drive resistance along suppressed anti-tumor immunity, treatment-refractory HNSCC tumors may retain sensitivity to targeted agents secondary synergistic lethality other oncogenic drivers (e.g., NOTCH1 mutations). We evaluated efficacy PI3K inhibitors (PI3Ki) bypassing Nrf2-mediated HNSCC....
Whole transcriptome sequencing (WTS/ RNA-Seq) is a ubiquitous tool for investigating cancer biology. RNA isolated from frozen sources limits possible studies analysis of associations with phenotypes or clinical variables requiring long-term follow-up. Although good correlations are reported in RNA-Seq data paired and formalin fixed paraffin embedded (FFPE) samples, uncertainties regarding quality, methods extraction, reliability hurdles to utilization archival samples. We compared three...
The TMPRSS2/ERG (T/E) fusion gene is present in the majority of all prostate cancers (PCa). We have shown previously that NF-kB signaling highly activated these T/E expressing cells via phosphorylation p65 Ser536 (p536). therefore hypothesize targeting may be an efficacious approach for subgroup PCas carry fusions. Celastrol a well known inhibitor, and thus inhibit PCa cell growth. evaluated Celastrol's effects vitro vivo VCaP cells, which express gene. were treated with different...
Benign prostatic hyperplasia (BPH) is characterized by increased tissue mass in the transition zone of prostate, which leads to obstruction urine outflow and considerable morbidity a majority older men. Senescent cells accumulate human tissues, including with increasing age. Expression proinflammatory cytokines these senescent cells, manifestation senescence-associated secretory phenotype. Multiplex analysis revealed that multiple are BPH, GM-CSF, IL-1α, IL-4, also epithelial vitro. Tissue...
Abstract African American (AA) men exhibit a relatively high incidence and mortality due to prostate cancer even after adjustment for socioeconomic factors, but the biological basis this disparity is unclear. Here, we identify novel region on chromosome 4p16.3 that lost selectively in AA cancer. The negative regulator of G-protein signaling RGS12 was defined as target deletions, although it has not been implicated previously tumor-suppressor gene. transcript levels were reduced cancer, cell...
African American (AA) men have a higher incidence and significantly mortality rates from prostate cancer than white men, but the biological basis for these differences are poorly understood. Few studies been carried out to determine whether there areas of allelic loss or gain in cancers AA that over-represented specific this group. To better understand molecular mechanisms we analyzed 20 with high-density single-nucleotide polymorphism arrays detect genomic copy number alterations. We...
BACKGROUND. African American patients have higher cancer mortality rates and shorter survival times compared with European patients. Despite a significant focus on socioeconomic factors, recent findings strongly argue the existence of biological factors driving this disparity. Most these been described in cancer-type specific context rather than pan-cancer setting.
Abstract Mechanisms of lung squamous cell carcinoma (LSCC) development are poorly understood. Here, we report that JNK1/2 activities attenuate Lkb1 -deficiency-driven LSCC initiation and progression through repressing ΔNp63 signaling. In vivo ablation alone is sufficient to induce by reducing MKK7 levels activities, independent the AMPKα mTOR pathways. positively regulated during development. Pharmaceutically elevated abates dependent formation while compound mutations Jnk1/2 further...
The regenerative potential of the endometrium is attributed to endometrial stem cells; however, signaling pathways controlling its remain obscure. In this study, genetic mouse models and organoids are used demonstrate that SMAD2/3 controls regeneration differentiation. Mice with conditional deletion in uterine epithelium using Lactoferrin-iCre develop hyperplasia at 12-weeks metastatic tumors by 9-months age. Mechanistic studies determine or pharmacological inhibition disrupts organoid...