A5069800720- Cancer, Lipids, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Bladder and Urothelial Cancer Treatments
- Cancer, Hypoxia, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Brain Metastases and Treatment
- Drug Transport and Resistance Mechanisms
- Liver Disease Diagnosis and Treatment
- Digestive system and related health
- RNA modifications and cancer
- Nanoplatforms for cancer theranostics
- Diet and metabolism studies
- Immunotherapy and Immune Responses
- Metabolism and Genetic Disorders
- Gut microbiota and health
- Chemical Reactions and Isotopes
- Esophageal Cancer Research and Treatment
- Click Chemistry and Applications
- Amino Acid Enzymes and Metabolism
- Birth, Development, and Health
- Radiopharmaceutical Chemistry and Applications
- Cancer-related gene regulation
- Pharmacogenetics and Drug Metabolism
Baylor College of Medicine
2017-2025
Weatherford College
2024
Dan L Duncan Comprehensive Cancer Center
2018
Abstract There is a critical need to improve our understanding of the pathogenesis melanoma brain metastases (MBM). Thus, we performed RNA sequencing on 88 resected MBMs and 42 patient-matched extracranial metastases; tumors with sufficient tissue also underwent whole-exome sequencing, T-cell receptor IHC. demonstrated heterogeneity immune infiltrates that correlated prior radiation post-craniotomy survival. Comparison identified significant immunosuppression enrichment oxidative...
Abstract Purpose: The perturbation of metabolic pathways in high-grade bladder cancer has not been investigated. We aimed to identify a signature by integrating unbiased metabolomics, lipidomics, and transcriptomics predict patient survival discover novel therapeutic targets. Experimental Design: performed high-resolution liquid chromatography mass spectrometry (LC-MS) bioinformatic analysis determine the global metabolome lipidome cancer. further investigated effects impaired using vitro...
Epithelial-mesenchymal transition (EMT) contributes significantly to interstitial matrix deposition in diabetic kidney disease (DKD). However, detection of EMT tissue is impracticable, and anti-EMT therapies have long been hindered. We reported that phosphatase tensin homolog (PTEN) promoted transforming growth factor beta 1 (TGF-β), sonic hedgehog (SHH), connective (CTGF), interleukin 6 (IL-6), hyperglycemia-induced when PTEN was modified by a MEX3C-catalyzed K27-linked polyubiquitination...
Abstract Avascular necrosis of the femur head (AVNFH) is a debilitating disease caused due to use alcohol, steroids, following trauma or unclear (idiopathic) etiology, affecting mostly middle aged population. Clinically AVNFH associated with impaired blood supply femoral resulting in bone and collapse. Although Homocysteine (HC) has been implicated AVNFH, levels homocysteine its pathway metabolites have not characterized. We demonstrate elevated concomitantly reduced vitamins B 6 12 , plasma...
Background African Americans (AAs) experience a disproportionally high rate of bladder cancer (BLCA) deaths even though their incidence rates are lower than those other patient groups. Using metabolomics approach, this study investigated how AA BLCA may differ molecularly from European (EAs) BLCA, and it examined serum samples patients with the aim identifying druggable metabolic pathways in patients. Methods Targeted was applied to measure more 300 metabolites 2 independent cohorts EA...
Abstract Our early-life environment has a profound influence on developing organs that impacts metabolic function and determines disease susceptibility across the life-course. Using rat model for exposure to an endocrine disrupting chemical (EDC), we show causes dysfunction in adulthood reprograms histone marks liver accelerate acquisition of adult epigenomic signature. This reprogramming persists long after initial exposure, but many reprogrammed genes remain transcriptionally silent with...
The first global lipidomic profiles associated with urothelial cancer of the bladder (UCB) and its clinical stages progression were identified.
Abstract Smoking is a major risk factor for the development of bladder cancer; however, functional consequences carcinogens in tobacco smoke and cancer–associated metabolic alterations remain poorly defined. We assessed profiles cancer smokers non-smokers identified key their metabolism. LC/MS bioinformatic analysis were performed to determine metabolome associated with further validated cell line models. Smokers found have elevated levels methylated metabolites, polycyclic aromatic...
Abstract Background: The current system to predict the outcome of smokers with bladder cancer is insufficient due complex genomic and transcriptomic heterogeneities. This study aims identify serum metabolite-associated genes related survival in this population. Methods: We performed LC/MS-based targeted metabolomic analysis for >300 metabolites obtained from two independent cohorts never smokers, healthy smokers. A subset differential was validated using Biocrates absoluteIDQ p180...
Metabolomics is a discovery tool for novel associations of metabolites with disease. Here, we interrogated the metabolome human breast tumors to describe whose accumulation affects tumor biology.We applied large-scale metabolomics followed by absolute quantification and machine learning-based feature selection using LASSO identify that show robust association biology disease outcome. Key observations were validated analysis an independent dataset cell culture experiments.LASSO-based revealed...
For patients with head and neck squamous cell carcinoma (HNSCC), failure of definitive radiation combined cisplatin nearly universally results in death. Although hyperactivation the Nrf2 pathway can drive resistance along suppressed anti-tumor immunity, treatment-refractory HNSCC tumors may retain sensitivity to targeted agents secondary synergistic lethality other oncogenic drivers (e.g., NOTCH1 mutations). We evaluated efficacy PI3K inhibitors (PI3Ki) bypassing Nrf2-mediated HNSCC....
The differentiation and suppressive functions of regulatory CD4 T cells (Tregs) are supported by a broad array metabolic changes, providing potential therapeutic targets for immune modulation. In this study, we focused on the role glycolytic enzymes in Tregs identified phosphoglycerate mutase (PGAM) as being differentially overexpressed associated with highly phenotype. Pharmacologic or genetic inhibition PGAM reduced Treg function while reciprocally inducing markers pro-inflammatory, helper...
The differentiation and suppressive functions of regulatory CD4 T cells (Tregs) are supported by a broad array metabolic changes, providing potential therapeutic targets for immune modulation. In this study, we focused on the role glycolytic enzymes in Tregs identified phosphoglycerate mutase (PGAM) as being differentially overexpressed associated with highly phenotype. Pharmacologic or genetic inhibition PGAM reduced Treg function while reciprocally inducing markers pro-inflammatory, helper...
Abstract Background Exposomes are critical drivers of carcinogenesis. However, how they modulate tumor behavior remains unclear. Extensive clinical data show cigarette smoke to be a key exposome that promotes aggressive tumors, higher rates metastasis, reduced response chemoradiotherapy, and suppressed anti-tumor immunity. We sought determine whether itself can in head neck squamous cell carcinoma (HNSCC) through reprogramming the cellular reductive state. Methods Using established human...
// Venkatrao Vantaku 1, * , Sri Ramya Donepudi 2, Chandrashekar R. Ambati Feng Jin 2 Vasanta Putluri Khoa Nguyen 3 Kimal Rajapakshe 1 Cristian Coarfa Venkata Lokesh Battula Yair Lotan 4 and Nagireddy Department of Molecular Cell Biology, Baylor College Medicine, Houston, TX, USA Dan L. Duncan Cancer Center, Advanced Technology Core, Alkek Center for Discovery, Section Hematology Therapy, Leukemia, Breast Medical Oncology, The University Texas MD Anderson Urology, Southwestern, Dallas, These...
ABSTRACT Cells are internally organized into compartmentalized organelles that execute specialized functions. To understand the functions of individual and their regulations, it is critical to resolve compositions organelles, which relies on a rapid efficient isolation method for specific organellar populations. Here, we introduce robust affinity purification intracellular (e.g. lysosomes, mitochondria peroxisomes) by taking advantage extraordinarily high between twin strep tag streptavidin...
Abstract Autophagy is a degradative pathway for removing aggregated proteins, damaged organelles, and parasites. Evidence indicates that autophagic pathways differ between cell types. In neurons, autophagy plays homeostatic role, compared to survival mechanism employed by starving non-neuronal cells. We investigated if sphingosine kinase 1 (SK1)-associated differs two symbiotic brain types—neurons astrocytes. SK1 synthesizes sphingosine-1-phosphate, which regulates in cells neurons. found...
Abstract African American (AA) men have a 60% higher incidence and two times greater risk of dying prostate cancer (PCa) than European men, yet there is limited insight into the molecular mechanisms driving this difference. To our knowledge, metabolic alterations, cancer-associated hallmark, not been reported in AA PCa, despite their importance tumor biology. Therefore, we measured 190 metabolites across ancestry-verified PCa/benign adjacent tissue pairs (n = 33 each) identified alterations...
Malonic acid (MA), methylmalonic (MMA), and ethylmalonic (EMA) metabolites are implicated in various non-cancer disorders that associated with inborn-error metabolism. In this study, we have slightly modified the published 3-nitrophenylhydrazine (3NPH) derivatization method applied it to derivatize MA, MMA, EMA their hydrazone derivatives, which were amenable for liquid chromatography- mass spectrometry (LC-MS) quantitation. 3NPH was used EMA, multiple reaction monitoring (MRM) transitions...
Methylation aberrations play an important role in many metabolic disorders including cancer.
Racial/ethnic disparities have a significant impact on bladder cancer outcomes with African American patients demonstrating inferior survival over European-American patients. We hypothesized that epigenetic difference in methylation of tumor DNA is an underlying cause this health disparity. analyzed tumors from and using reduced representation bisulfite sequencing (RRBS) to annotate differentially methylated regions. Liquid chromatography-mass spectrometry (LC-MS/MS) based metabolomics flux...
To assess the ability of signature metabolites alone, or in combination with model for end-stage liver disease-Na (MELD-Na) score to predict mortality patients cirrhosis caused by primary biliary cholangitis sclerosing cholangitis.Plasma were detected using ultrahigh-performance liquid chromatography/tandem mass spectrometry 39 cholangitis. Mortality was predicted Cox proportional hazards regression and time-dependent receiver operating characteristic curve analyses.The top five...