A5019445691- Research on Leishmaniasis Studies
- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Trypanosoma species research and implications
- Plant Pathogens and Fungal Diseases
- Nail Diseases and Treatments
- Toxoplasma gondii Research Studies
- Parasites and Host Interactions
- Pneumocystis jirovecii pneumonia detection and treatment
- Infectious Diseases and Mycology
- Herpesvirus Infections and Treatments
- Synthesis and Biological Evaluation
- Cytomegalovirus and herpesvirus research
- Cutaneous lymphoproliferative disorders research
- Mosquito-borne diseases and control
- Parasitic Infections and Diagnostics
- Malaria Research and Control
- Amoebic Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Autoimmune Bullous Skin Diseases
- Antimicrobial Resistance in Staphylococcus
- Digital Imaging for Blood Diseases
- Asthma and respiratory diseases
- Entomopathogenic Microorganisms in Pest Control
- Sinusitis and nasal conditions
Centre Hospitalier Universitaire de Montpellier
2016-2025
Université de Montpellier
2014-2024
Maladies Infectieuses et Vecteurs: Écologie, Génétique, Évolution et Contrôle
2012-2023
Agropolis International
2013-2023
Institut de Recherche pour le Développement
2010-2022
Centre National de la Recherche Scientifique
2010-2022
Hôpital Lapeyronie
2021
Centre Hospitalier Universitaire de Nîmes
2009-2018
Laboratoire National de Référence
2000-2010
Zero to Three
2010
Abstract Alterations in the composition of commensal bacterial populations, a phenomenon known as dysbiosis, are linked to multiple gastrointestinal disorders, such inflammatory bowel disease and irritable syndrome, or infections by diverse enteric pathogens. Blastocystis is one most common single-celled eukaryotes detected human faecal samples. However, clinical significance this widespread colonization remains unclear, its pathogenic potential controversial. To address issue pathogenicity,...
ABSTRACT The objectives of this study were to compare the sensitivities and reliabilities different PCR methods for diagnosis epidemiological canine visceral leishmaniasis (CVL) using dog blood. We chose work with peripheral blood, as type sampling is noninvasive, straightforward, easy repeat. Six compared: three primer pairs target genomic DNA, other kinetoplast (mitochondrial) DNA. Sensitivity, specificity, reproducibility, ease interpretation without hybridization evaluated each method....
The protozoan parasite Leishmania is generally considered to be diploid, although a few chromosomes have been described as aneuploid. Using fluorescence in situ hybridization (FISH), we determined the number of homologous per individual cell L. major (i) during interphase and (ii) mitosis. We show that, Leishmania, aneuploidy appears rule, it affects all that studied. Moreover, every chromosome was observed at least two ploidy states, among monosomic, disomic or trisomic, population. This...
Blastocystis sp. is the most common intestinal parasite of humans. Despite its potential public health impact, epidemiological data regarding prevalence and molecular subtype distribution in Europe are rarely reported. Therefore, first multi-center survey performed was conducted France to diagnose identify risk factors for infection. Stool samples from 788 patients were collected either summer or winter 11 hospitals throughout together with patient data. All stool tested presence by...
During the last decade miltefosine (MIL) has been used as first-line treatment for visceral leishmaniasis in endemic areas with antimonial resistance, but a decline clinical effectiveness is now being reported. While only two MIL-resistant Leishmania infantum strains from HIV co-infected patients have documented, phenotypic MIL-resistance L. donovani not yet identified laboratory. Hence, better understanding of factors contributing to increased MIL-treatment failure necessary. Given paucity...
ABSTRACT We developed a highly sensitive PCR method that enables the diagnosis and posttherapeutic follow-up of visceral leishmaniasis with patient blood. The assay was thoroughly optimized by successive procedural refinements to increase its sensitivity specificity. It compared in vitro cultivation as well direct examination bone marrow serology. Two hundred thirty-seven patients presenting clinical signs compatible were included study. Thirty-six diagnosed having Mediterranean (MVL)....
The value of 2 PCR methods, targeting genomic and kinetoplast minicircle DNA respectively, was investigated for both diagnosis prevalence studies canine visceral leishmaniasis (CVL). first method (R) 5000-fold less sensitive than the second (method KRV). Both were tested CVL in 44 sick dogs with confirmed disease using different biological samples. Method R highly efficient when invasive samples, but use KRV proved necessary a 100% peripheral blood. This applied to blood skin samples 263...
Visceral leishmaniasis (VL) is an opportunistic infection that can occur among patients infected with human immunodeficiency virus type 1 (HIV-1) in areas where both infections are endemic. Highly active antiretroviral therapy has decreased the incidence of VL southern Europe HIV-1-infected patients, but still observed low CD4 cell counts, and most coinfected receiving highly experienced relapse, despite initial treatment liposomal amphotericin B.Through long-term monitoring 10 HIV-1 and/or...
We have compared various sample preparation methods for the PCR diagnosis of visceral leishmaniasis (VL) using peripheral blood samples and tested influence these protocols upon sensitivity. Four lysis-DNA extraction were used with two types samples: whole (WB) buffy coat (BC). Comparisons first carried out seeded at parasite concentrations. At high concentrations (> or = 1,000 parasites/ml), there no significant differences in sensitivity among tested. < 100 parasites/ml, proteinase K...
Although miltefosine and paromomycin were only recently introduced to treat visceral leishmaniasis, increasing numbers of treatment failures occasional primary resistance both drugs have been reported. Understanding alterations in parasite behaviour linked drug is essential assess the propensity for emergence spread resistant strains, particularly since a positive effect on fitness has reported antimony-resistant parasites. This laboratory study compared drug-susceptible parent WT clinical...
The taxonomy of Aspergillus species has recently been revolutionized with the introduction cryptic and section concepts. However, their species-level identification in routine laboratories remains a challenge. aim this study was to prospectively assess accuracy various using mass spectrometry (MSI) platform, an independent freely accessible online database. Over 12-month period, when select set MSI users identified species, they were contacted requested send isolates our laboratory for...
Abstract Background While invasive fusariosis and lomentosporiosis are known to be associated with fungemia, overall data on mold-related fungemia limited, hampering early management. This study aimed describe the epidemiology of mold-positive blood cultures. Methods Epidemiological clinical cultures from 2012 2022 were obtained RESSIF database. Pseudofungemia was excluded using modified Duthie Denning criteria. Univariable multivariable Firth logistical regression used factors 90-day...
ABSTRACT Brazil faces unique challenges in managing invasive fungal infections (IFIs) due to diverse ecosystems, a rural workforce, and prevalent health conditions. In Europe, IFIs are primarily associated with transplantation, intensive care, chronic diseases. Inspired by initiatives the Caribbean Latin America 2019, efforts map global diagnostic treatment resources expanded Africa, Asia/Pacific. This study conducts comparative analysis, mainly drawing data from investigate IFI epidemiology...
ABSTRACT The PCR diagnosis of toxoplasmosis suffers from lack standardization. Interlaboratory comparative studies methods have been performed, but intralaboratory comparisons are scarce. Here, we optimized and compared the technical performances two primer systems widely used for Toxoplasma detection. differences between were visible only at low parasite concentrations (≤1 genome per reaction tube). Nevertheless, when clinical samples tested, both significantly differed in their...
Objectives The aim of the study was to describe a new evolutionary form visceral leishmaniasis observed in immunocompromised patients. Methods We carried out long‐term clinical and biological follow‐up 10 HIV‐1/ Leishmania ‐coinfected patients presenting numerous secondary episodes despite treatment, with time ranging from 0.5 years. Results Analysis polymerase chain reaction (PCR) blood culture results demonstrated continuous multiplication circulation parasites both during asymptomatic...
Background. Cutaneous leishmaniasis (CL) is a disfiguring but not life-threatening disease. Because antileishmanial drugs are potentially toxic, the World Health Organization (WHO) recommends simple wound care or local therapy as first-line treatment, followed replaced by systemic if fails cannot be performed. Methods. To determine feasibility and impact of recommended approach, we analyzed results centralized referral treatment program in 135 patients with parasitologically proven CL....