A5079960493- Immune cells in cancer
- Stress Responses and Cortisol
- Immune Response and Inflammation
- Adenosine and Purinergic Signaling
- Inflammasome and immune disorders
- Mesenchymal stem cell research
- Genetics and Neurodevelopmental Disorders
- Cancer, Hypoxia, and Metabolism
- Anesthesia and Neurotoxicity Research
- Bipolar Disorder and Treatment
- Ubiquitin and proteasome pathways
- Liver Disease Diagnosis and Treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pancreatic function and diabetes
- Respiratory Support and Mechanisms
- Intensive Care Unit Cognitive Disorders
- Fatty Acid Research and Health
- Genetic and Kidney Cyst Diseases
- PI3K/AKT/mTOR signaling in cancer
- RNA modifications and cancer
- Tannin, Tannase and Anticancer Activities
- Diet, Metabolism, and Disease
- DNA Repair Mechanisms
- Genomics, phytochemicals, and oxidative stress
- Liver physiology and pathology
Institut d'Investigació Biomédica de Bellvitge
2016-2023
Universitat de Barcelona
2016-2023
Pontifícia Universidade Católica do Rio Grande do Sul
2007-2022
Bellvitge University Hospital
2020-2021
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are syndromes of hypoxemic failure resulting from a variety direct indirect injuries to the gas exchange parenchyma lungs. During ALI, we have an increase release proinflammatory cytokines high reactive oxygen species (ROS) formation. These factors responsible for activation neutrophil‐derived proteases formation neutrophil extracellular traps (NETs). The excessive in NETs cause damage tissue. Recent studies involving...
Osteocytes, the most abundant bone cell type, are derived from osteoblasts through a process in which they embedded an osteoid. We previously showed that nutrient restriction promotes osteocyte transcriptional program and is associated with increased mitochondrial biogenesis. Here, we show biogenesis increase reactive oxygen species (ROS) levels consequently, NRF2 activity during osteocytogenesis. osteocyte-specific expression of Dmp1, Mepe, Sost IDG-SW3 cells, primary osteocytes,...
Sepsis is a severe medical condition that ranks among the top 10 causes of death worldwide and which has permanently high incidence rates. Mesenchymal stem cells (MSCs) have been found to be potent modulators immune responses. More importantly, there evidence MSCs beneficial effect on preclinical models polymicrobial sepsis. However, changes caused by in effector host system remain unclear. A mouse model sepsis (male C57BL/6 mice) with three experimental groups was used for experiments vivo:...
Apoptosis and NETosis of neutrophils are two major mechanisms programmed cell death that differ in their morphological characteristics effects on the immune system. can be delayed by presence pathogens or chemical components such as lipopolysaccharide (LPS). Neutrophils have other antimicrobial strategy, called neutrophil extracellular traps (NETs), which contributes to elimination control pathogen. is induced infection, inflammation trauma represents an innate activation mechanism. The...
Sepsis is a major health care problem, with significant mortality rate in intensive units. We evaluated biochemical and inflammatory markers patients severe sepsis septic shock its association of rates.Critically ill diagnoses - group (n=23) (n=25), control (n=17) were recruited within 24h entry into the ICU. Serum levels mediators measured (IL-1β, IL-6, IL-8, IL-10, TNF-α, IL-18 nitric oxide). have also collected clinical parameters laboratorial tests to estimate severity organ dysfunction...
A mutation in the HERC2 gene has been linked to a severe neurodevelopmental disorder with similarities Angelman syndrome. This codifies protein ubiquitin ligase activity that regulates of tumor p53 and is involved important cellular processes such as DNA repair, cell cycle, cancer, iron metabolism. Despite critical role these physiological pathological processes, little known about its relevance vivo. Here, we described mouse targeted inactivation Herc2 gene. Homozygous mice were not viable....
Abstract Protein modifications by phosphorylation or ubiquitylation have been selected throughout evolution as efficient regulatory mechanisms of cellular processes. Cell migration is a complex, highly coordinated process where these must participate in an integrated manner to transmit signaling during migration. In this study, we show that the ubiquitin ligase HERC1 regulates p38 pathway, and regulation mediated MAPK kinase MKK3. Moreover, demonstrate crosstalk between RAF MKK3/p38 pathways...
Metabolic syndrome (MetS) is a group of risk factors that increase the for heart disease. Little known about role IL-10 in severity coronary artery disease (CAD) patients with MetS. We investigated plasma levels and other pro-inflammatory cytokines MetS or without severe CAD. Cross-sectional study healthy individuals. interleukins were analyzed 90 subjects divided into 3 groups: 1 (n = 30), CAD; 2 CAD (history myocardial infarction revascularization performed through surgery percutaneous...
Abstract Bone remodeling is a continuous process between bone-forming osteoblasts and bone-resorbing osteoclasts, with any imbalance resulting in metabolic bone disease, including osteopenia. The HERC1 gene encodes an E3 ubiquitin ligase that affects cellular processes by regulating the ubiquitination of target proteins, such as C-RAF. Of interest, association exists biallelic pathogenic sequence variants neurodevelopmental disorder MDFPMR syndrome (macrocephaly, dysmorphic facies,...
Abstract HERC1 is a ubiquitin ligase protein, which, when mutated, induces several malformations and intellectual disability in humans. The animal model of mutation the mouse tambaleante characterized by: (1) overproduction protein; (2) cerebellar Purkinje cells death by autophagy; (3) dysregulation autophagy spinal cord motor neurons, CA3 neocortical pyramidal neurons; (4) impairment associative learning, linked to altered spinogenesis absence LTP lateral amygdala; and, (5) due delayed...
Septic shock presents as a continuum of infectious events, generating tissue hypoxia and hypovolemia, increased oxidative stress. Chest physiotherapy helps reduce secretion, improving dynamic static compliance, well secretion clearance preventing pulmonary complications. The purpose this study was to evaluate the immediate effect chest on hemodynamic, metabolic, inflammatory, stress parameters in subjects septic shock.We conducted quasi-experimental 30 shock, who underwent physiotherapy,...
As the population ages, osteometabolic diseases and osteoporotic fractures emerge, resulting in substantial healthcare resource utilization impaired quality of life. Many types mechanical stimulation have potential being recognized by bone cells after a sign is transformed into biological one (a process called mechanotransduction). The therapeutic ultrasound (TU) several resources capable promoting cell stimulation. Therefore, main purpose present study was to evaluate effect TU on...
HERC2 gene encodes an E3 ubiquitin ligase involved in several cellular processes by regulating the ubiquitylation of different protein substrates. Biallelic pathogenic sequence variants are associated with Angelman-like syndrome. In variants, complete absence or marked reduction levels observed. The most common pathological variant, c.1781C > T (p.Pro594Leu), unstable protein. A better understanding how pathologic affect intracellular signalling may aid definition potential new therapies for...
The incidence of hepatocellular carcinoma (HCC) keeps rising year by year, and became the second leading cause cancer-related death. Some studies have found that liraglutide, a GLP-1 analog, may decrease tumor cells proliferation. Due to this, aim this work is investigate antiproliferative potential exenatide, another analog. Cell proliferation was assessed direct count with Trypan blue dye exclusion. Flow cytometry used determinate autophagy nuclear staining. Morphometric analysis verify...