A5022382649- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- T-cell and B-cell Immunology
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Asthma and respiratory diseases
- Long-Term Effects of COVID-19
- Cancer Immunotherapy and Biomarkers
- Immunodeficiency and Autoimmune Disorders
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- SARS-CoV-2 detection and testing
- Respiratory Support and Mechanisms
- Mast cells and histamine
- Metabolism, Diabetes, and Cancer
- Diabetes Treatment and Management
- Influenza Virus Research Studies
- Renal Transplantation Outcomes and Treatments
- Immune cells in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Heparin-Induced Thrombocytopenia and Thrombosis
- vaccines and immunoinformatics approaches
- Genomics, phytochemicals, and oxidative stress
Massachusetts General Hospital
2021-2024
Harvard University
2020-2024
Pontifícia Universidade Católica do Rio Grande do Sul
2015-2021
Brigham and Women's Hospital
2020-2021
Instituto do Cérebro
2017
Universidade Franciscana
2015
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are syndromes of hypoxemic failure resulting from a variety direct indirect injuries to the gas exchange parenchyma lungs. During ALI, we have an increase release proinflammatory cytokines high reactive oxygen species (ROS) formation. These factors responsible for activation neutrophil‐derived proteases formation neutrophil extracellular traps (NETs). The excessive in NETs cause damage tissue. Recent studies involving...
Long-term organ transplant survival remains suboptimal, and life-long immunosuppression predisposes recipients to an increased risk of infection, malignancy, kidney toxicity. Promoting the regulatory arm immune system by expanding Tregs may allow minimization improve long-term graft outcomes. While low-dose IL-2 treatment can expand Tregs, it has a short half-life off-target expansion NK effector T cells, limiting its clinical applicability. Here, we designed humanized mutein with high Treg...
COVID-19 manifests as a milder disease in children than adults, but the underlying mechanisms are not fully characterized. Here we assess difference cellular or humoral immune responses of pediatric and adult patients to see if these factors contribute severity dichotomy. Children's non-specific profile is dominated by naive lymphocytes HLA-DR
Abstract In asthma, there are high levels of inflammatory mediators, reactive oxygen species (ROS), and eosinophil extracellular traps (EETs) formation in airway. Here, we attempted to investigate the ROS involvement EETs release airway inflammation OVA‐challenged mice. Before intranasal challenge with ovalbumin (OVA), animals were treated two inhibitors, N ‐acetylcysteine (NAC) or diphenyleneiodonium (DPI). We showed that NAC treatment reduced cells lung. DPI treatments peroxidase (EPO),...
Abstract Studies have shown autophagy participation in the immunopathology of inflammatory diseases. However, role asthma and eosinophil extracellular traps (EETs) release is poorly understood. Here, we attempted to investigate involvement EETs lung inflammation an experimental model. Mice were sensitized with ovalbumin (OVA), followed by OVA challenge. Before challenge OVA, mice treated inhibitor, 3‐methyladenine (3‐MA). We showed that 3‐MA treatment decreases number eosinophils, peroxidase...
Introduction Studies have shown reduced antiviral responses in kidney transplant recipients (KTRs) following SARS-CoV-2 mRNA vaccination, but data on post-vaccination alloimmune and against the Delta (B.1.617.2) variant are limited. Materials methods To address this issue, we conducted a prospective, multi-center study of 58 adult KTRs receiving mRNA-BNT162b2 or mRNA-1273 vaccines. We used multiple complementary non-invasive biomarkers for rejection monitoring including serum creatinine,...
It has been reported that glucagon-like peptide-1 (GLP-1) agents have associated with both the increased risk of cancer and inhibition tumor growth metastases. The aim this study is to evaluate effect liraglutide on hepatocellular carcinoma cells - HepG2. Cytometry was used mechanism related decreased cell proliferation. Nuclear staining morphometric analysis were also verify process taking place after treatment liraglutide, in order better understand mechanism, TGF-β1 performed. HepG2...
The programmed death 1/programmed ligand 1 (PD-1/PD-L1) pathway is a potent inhibitory involved in immune regulation and potential therapeutic target transplantation. In this study, we show that overexpression of PD-1 on T cells (PD-1 Tg) promotes allograft tolerance fully MHC-mismatched cardiac transplant model when combined with costimulation blockade CTLA-4–Ig. also protected against chronic rejection single MHC II–mismatched model, whereas the still allowed generation an effective...
Respiratory viral infections are the leading cause of asthma exacerbations. Eosinophil activation results in formation eosinophil extracellular traps (EETs), which release web-like structures DNA and proteins that bind, disarm extracellularly kill pathogens.We investigated whether respiratory syncytial virus (RSV) vitro could induce EETs bronchoalveolar lavage fluid eosinophils a murine model asthma.BALB/cJ mice (6-8 weeks old) were sensitized with 2 subcutaneous injections ovalbumin (20 μg)...
Abstract Background Available SARS-CoV-2 vaccines have reduced efficacy against the Omicron variant in immunocompetent individuals. Kidney transplant recipients (KTRs) diminished antiviral responses to wild-type after vaccination, and data on variants, including variant, are limited. Methods We conducted a prospective, multi-center cohort study of 51 adult KTRs who received three doses BNT162b2 or mRNA-1273. Blood urine samples were collected before four weeks third vaccine dose. The primary...
Limb transplantation is a life-changing procedure for amputees. However, limb recipients have 6-fold greater rejection rate than solid organ transplant recipients, related in part to immunogenicity of the skin. Here, we report detailed immunological and molecular characterization individuals who underwent bilateral at our institution. Circulating Th17 cells are increased over time. Molecular 770 genes skin biopsies reveals upregulation T cell effector immune molecules chemokines,...
Respiratory syncytial virus (RSV) is the major cause of lower respiratory tract infections in children under 1 year. RSV vaccines are currently unavailable, and suffering from multiple reinfections by same viral strain fail to develop protective responses. Although RSV-specific antibodies can be detected upon infection, these have limited neutralizing capacity. Follicular helper T (Tfh) cells specialized providing signals B help production affinity maturation antibodies, mainly via...
The incidence of hepatocellular carcinoma (HCC) keeps rising year by year, and became the second leading cause cancer-related death. Some studies have found that liraglutide, a GLP-1 analog, may decrease tumor cells proliferation. Due to this, aim this work is investigate antiproliferative potential exenatide, another analog. Cell proliferation was assessed direct count with Trypan blue dye exclusion. Flow cytometry used determinate autophagy nuclear staining. Morphometric analysis verify...
Leucine (Leu) is an essential branched-chain amino acid, present in dairy products, which has been investigated for its important role cell signaling. The effects of Leu on several kinds cells have studied, altough little known action upon bone and proliferation. Thus, the aim this study to investigate supplementation proliferation pre-osteoblasts from MC3T3-E1 lineage. were kept Alpha medium supplemented with 10% fetal bovine serum 1% antibiotic-antimitotic. Cells treated during 48 h by...
Purpose Eosinophils are one of the main cells responsible to inflammatory response in asthma by release molecules such as cytokines, reactive oxygen species (ROS), cytotoxic granule, eosinophil extracellular trap (EET), and lipid mediators cysteinyl leukotriene (cysLT). The interconnections between these not fully understood. Here, we attempted investigate cysLT participation mechanisms EET formation an model OVA challenge.Materials methods Before intranasal challenge with OVA, BALB/cJ mice...