A5076313042- Reproductive Biology and Fertility
- Advanced biosensing and bioanalysis techniques
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- COVID-19 Clinical Research Studies
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Winter Sports Injuries and Performance
- Long-Term Effects of COVID-19
- Renal and related cancers
- Ovarian function and disorders
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- Liver Disease Diagnosis and Treatment
- Collaboration in agile enterprises
- Diet, Metabolism, and Disease
- Lymphoma Diagnosis and Treatment
- Welding Techniques and Residual Stresses
- Epigenetics and DNA Methylation
- Mesenchymal stem cell research
- Non-Destructive Testing Techniques
- Chronic Lymphocytic Leukemia Research
- Traditional Chinese Medicine Studies
- Hydrogen embrittlement and corrosion behaviors in metals
- Biological Research and Disease Studies
Ningxia Medical University
2017-2025
Zhoushan Hospital
2025
Ningxia Medical University General Hospital
2024
Huazhong University of Science and Technology
2021-2024
Shanxi Medical University
2023
Chinese PLA General Hospital
2021-2023
Hunan Agricultural University
2022-2023
Hunan Normal University
2023
Changsha Hospital for Maternal and Child Health Care
2023
Qingdao University
2021-2022
Treatments for coronavirus disease 2019 (COVID-19) are limited by suboptimal efficacy. From January 30, 2020 to March 23, 2020, we conducted a non-randomised controlled trial, in which all adult patients with laboratory-confirmed COVID-19 were assigned three groups non-randomly and given supportive treatments: Group A, Lopinavir-Ritonavir; B, Huashi Baidu Formula (a Chinese medicineformula made the China Academy of Medical Sciences treat COVID-19, is now clinical trial period) C, Formula....
Abstract Although CRISPR-Cas12a [clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 12a] combining pre-amplification technology has the advantage of high sensitivity in biosensing, its generality and specificity are insufficient, which greatly restrains application range. Here, we discovered a new targeting substrate for LbaCas12a (Lachnospiraceae bacterium Cas12a), namely double-stranded DNA (dsDNA) with sticky-end region (PAM−SE+ dsDNA). We that...
Diabetic renal injury was associated with dysbiosis of the gut microbiota and intestinal barrier. Punicalagin (PU) from pomegranates potentially impacts microbial ecosystem, barrier, function. Therefore, we hypothesized that PU may improve diabetic by modulating gut-kidney axis. The present study evaluated effect on axis kidney function in a mouse model induced high-fat diet (HFD). Mice were fed HFD without or at doses 50 100 mg kg-1 d-1 for 8 weeks. Targeted metabolomics GC-MS 16S rRNA...
Background: Previous research suggested that Chinese Medicine (CM) Formula Huashibaidu granule might shorten the disease course in coronavirus 2019 (COVID-19) patients. This aimed to investigate early treatment effect of well-managed patients with mild COVID-19. Methods: An unblinded cluster-randomized clinical trial was conducted at Dongxihu FangCang hospital. Two cabins were randomly allocated a CM or control group, 204 COVID-19 participants each cabin. All received conventional over 7 day...
The CRISPR-Cas12a system is a new type of genome editing tool with high efficiency and targeting. However, other sequences in the may also be cleaved nonspecifically, resulting unavoidable off-target effects. Therefore, it necessary to learn more about mechanism recognize target avoid its Here, we show that insertion (DNA bubble) or deletion (RNA dsDNA sequence compared crRNA sequence, can still cleave sequence. We conclude tolerance bubbles closely related location size bubble GC base...
Critical value (CV) management is vital for patient safety and shows the quality of critical care. This study aimed to develop a whole-chain system (WCMS) CV reporting evaluate its impact on clinical practice. A WCMS reporting, considering sample, process population, was developed. quasi-experimental conducted at Zhoushan Hospital. 591 CVs were divided into two groups: postapplication group (n = 298) preapplication 293). quality-related indicators compared between groups, including timely...
Apurinic/apyrimidinic endonuclease 1 (APE1) is a critical enzyme in the base excision repair (BER) pathway, essential for preserving cellular equilibrium. Variations APE1 activity within blood or tissues can provide significant insights clinical cancer screening and disease diagnosis. Consequently, detection of diagnostics. However, there currently deficiency rapid, straightforward, sensitive methods detection. To address this issue, we developed method that integrates Nicking Enzyme...
Abstract Cisplatin (CISP) is an efficacious anticancer agent used in chemotherapy, however, the constraint to its clinical utility stray organ toxicity including testicular damage linked oxidative and inflammatory cascades. This study aimed explore protective effect of nucleosides‐rich extract from Cordyceps cicadae (NRCE) against CISP‐induced rats. Rats were subjected prophylactic oral administration NRCE (50, 100 400 mg/kg body weight/day) for 7 days prior induced by CISP (10 mg/kg, ip)...
Abstract Dendrimers are a family of nano‐sized three‐dimensional polymers with unique dendritic branching structures and compact spherical geometries. In recent years, dendrimers have made series breakthroughs in the biomedical field. this review, we introduce synthesis principles, modification methods, new materials designed based on dendrimers; discuss importance cytotoxicity for applications; elaborate their applications field molecular assembly cancer diagnosis treatment. We speculate...
In this work, the protective effect of apple polyphenol extract (APE) on hepatic steatosis was investigated. Thirty-two C57BL/6J mice were assigned randomly to control group, lovastatin and APE group. After 8 weeks intervention, supplementation markedly decreased body weight gain, liver weight, index, epididymal adipose serum, lipid levels. Hematoxylin eosin staining revealed that alleviated histopathological changes steatosis. Western blot downregulated protein levels GRP78, IRE1α, p-IRE1α,...
The Polygonatum cyrtonema Hua rhizomes (also known as Rhizoma Polygonati, RP) are consumed for their health benefits. main source of the RP is wild P. populations in Hunan province China. However, soil Cadmium (Cd) content Huanan increasing, thus increasing risks Cd accumulation which may end up human food chain. To understand mechanism and resistance cyrtonema, we subjected plants to four levels stress [(D2) 1, (D3) 2, (D4) 4, (D5) 8 mg/kg)] compared (D1) 0.5 mg/kg.The increase 4 mg/kg...
With the development of cryopreservation technology, marked progress has been made regarding sperm cryopreservation. However, as conventional agents are not effective at freezing weak sperm, improved in demand. In present study, addition Lycium barbarum polysaccharides to glycerol-egg-yolk-citrate agent was determined improve forward speed, reduce DNA fragmentation index and mitochondrial membrane potential. Furthermore, during thawing cryopreservative increased content Bcl-2 while reducing...
Abstract Background Spermatogonial stem cell (SSC) transplantation technology as a promising option for male fertility preservation has received increasing attention, along with efficient SSC purification necessary technical support; however, the safety of such application in patients tumors remains controversial. Methods In this study, we used green fluorescent protein mouse xenograft model B acute lymphocytic leukemia. We isolated and purified SSCs from testicular tissue mice using density...
DNA methylation is closely related to cancer. It generally accepted that detection crucial in cancer diagnosis, prognosis, and treatment monitoring. Therefore, there an urgent demand for developing a simple, rapid, highly sensitive, specific method detect at sites quantitatively. In this work, we introduce based on MutS methylation-specific PCR, named MutS-based PCR (MB-MSP), which has the advantages of simplicity, speed, high specificity, sensitivity, broad applicability. Utilizing MutS's...
We aimed to determine the impact of high-fructose intake during pregnancy on fetal-placental unit in rats, which may be initial mechanism programming effect fructose. Pregnant Sprague–Dawley rats were randomly assigned three groups and respectively provided tap water (n = 10), 10% (w/v) fructose solution glucose 10) from embryonic day 0 20. Compared with control groups, significantly lower fetal length, weight, placental fetus/placenta ratio found group 20 (all p<0.05). In parallel markedly...
Gene mutations are important biomarkers for the diagnosis, classification, monitoring, and prognosis evaluation of cancers genetic diseases. Both personalized cancer treatment noninvasive prenatal testing require methods to accurately determine abundance mutation. At present, widely adopted convenient measuring mutation mainly based on relative quantification, which requires negative samples strict control analyte amounts. The development DNA-probe-based that can without nor amount is highly...
This study was aimed to investigate the efficacy of haploidentical hematopoietic stem cell transplantation (hi-HSCT) combined with umbilical cord mesenchymal cells (MSC) using modified conditioning regimen for treatment patients refractory and relapsed or high risk malignant hematologic diseases, clinical in 30 malignant, who voluntarily received HSCT analyzed. Among there were 4 cases 26 diseases. The above-mentioned included 15 AML, 9 ALL, 3 pro T lymphoblast lymphoma/leukemia, 1 spleen...
Objective To observe the clinical safety and efficacy of foscarnet prophylaxis pre-emptive therapy for cytomegalovirus (CMV) infection in allogeneic hematopoietic stem cell transplantation (allo-HSCT). Methods Ninety-six patients undergoing allo-HSCT from October 2014 to December 2016 were retrospectively analyzed. Plasma CMV-DNA was monitored with real-time quantitative polymerase chain reaction (RQ-PCR) beginning 180 days after transplantation. Foscarnet used not only but also...
The inhibitory effect of apurinic or apyrimidinic sites on the polymerase provides a novel principle for APE1 detection with high sensitivity.