A5101996344- Metabolomics and Mass Spectrometry Studies
- Ion Transport and Channel Regulation
- Electrolyte and hormonal disorders
- Magnesium in Health and Disease
- Liver Disease Diagnosis and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological Disease Mechanisms and Treatments
- Hepatitis C virus research
- Peroxisome Proliferator-Activated Receptors
- Organ Transplantation Techniques and Outcomes
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- T-cell and Retrovirus Studies
- Renal Transplantation Outcomes and Treatments
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Ion channel regulation and function
- Organ Donation and Transplantation
- NMR spectroscopy and applications
- Biomedical Research and Pathophysiology
- Renal function and acid-base balance
- Estrogen and related hormone effects
- Chronic Kidney Disease and Diabetes
- Vagus Nerve Stimulation Research
- Systemic Lupus Erythematosus Research
Aarhus University
1990-2014
Newcastle University
2002-2010
University of Münster
1990
Hypercalcemia is frequently associated with a urinary concentrating defect and overt polyuria. The molecular mechanisms underlying this are poorly understood. Dysregulation of aquaporin-2 (AQP2), the predominant vasopressin-regulated water channel, known to be range congenital acquired balance disorders including nephrogenic diabetes insipidus states retention. This study examines effect hypercalcemia on expression AQP2 in rat kidney. Rats were treated orally for 7 d dihydrotachysterol,...
Ultracentrifugation in sucrose density gradient remains the most commonly used technique for hRSV purification. However, high viscosity and hyper-osmotic property of can cause damage to extremely labile virus leading loss infectivity. To overcome these limitations, an alternative purification was developed using iodixanol as medium, incorporating MgSO(4) a stabilizing agent EDTA disaggregate prior infectivity assay. Virus particles were banded at 20-36% interface after polyethylene...
In models of genetic hypertension, renal tubular dysfunction could be involved in the increased sodium and water reabsorption. However, molecular basis for retention remains largely undefined spontaneously hypertensive rats (SHR). We hypothesized that dysregulation epithelial channels (ENaC), (co)transporters, or aquaporin-2 (AQP2) pathogenesis hypertension SHR. Six-week-old twelve-week-old SHR corresponding age-matched Wistar-Kyoto control (WKY) were studied. both groups, systolic blood...
Anti-inflammatory treatment affects ischemic damage and neurogenesis in rodent models of cerebral ischemia. We investigated the potential benefit COX-2 inhibition with parecoxib spontaneously hypertensive rats (SHRs) subjected to transient middle artery occlusion (tMCAo). Sixty-four male SHRs were randomized 90 min intraluminal tMCAo or sham surgery. Parecoxib (10 mg/kg) isotonic saline was administered intraperitoneally (IP) during procedure, twice daily thereafter. Nineteen animals...
Background: The effect of an α-melanocyte stimulating hormone (α-MSH) analogue (AP214) on experimentally endotoxin-induced systemic inflammatory response syndrome (SIRS) was studied, because α-MSH in rodent models has shown promise attenuating markers and associated organ damage SIRS. SIRS is with considerable morbidity mortality. Consequently, new treatment modalities are still warranted to address the different aspects pathophysiological process. Methods: induced by lipopolysaccharide...
Abstract A 100 000 g supernatant from human heart muscle, containing cytosolic proteins with some contaminating plasma proteins, was analyzed for fatty acid binding protein (FABP) by two‐dimensional electrophoresis (2‐DE) using isoelectric focusing under nondenaturing conditions in the first dimension. FABP purified muscle found to comigrate a major spot 2‐DE gels of supernatant. This comparable those myoglobins staining intensity. When charged [H]palmitate and subjected 2‐DE, radioactivity...
We are currently investigating microglial activation and neuronal precursor cell (NPC) proliferation after transient middle cerebral artery occlusion (tMCAo) in rats. This study aimed: (1) to investigate differences hippocampal NPC outbred male spontaneously hypertensive rats (SHRs) Sprague-Dawley (SDs) one week tMCAo; (2) present the practical use of optical fractionator 2D nucleator stereological brain tissue analyses; (3) report our experiences with an intraluminal tMCAo model where...
Serum, urine and tissue from a rat model of chronic kidney disease (CKD) were analysed using nuclear magnetic resonance (NMR) spectroscopy-based metabolomics methods, compared with samples sham operated rats. Both serum sampled at multiple timepoints, the results have been reported elsewhere (https://doi.org/10.1007/s11306-019-1569-3[1]). The data could be useful to researchers working human CKD or models disease. In addition, several different types NMR spectra recorded, including 1D NOESY,...
Abstract Aim Evidence suggests the possibility that pre‐existing chronic kidney ( CKD ) disease may result in a more severe outcome of acute injury AKI ). The aim this study was to examine whether enhances inflammatory response kidney, as well other organs, rats. Methods induced by 5/6 nephrectomy Nx and intestinal ischaemia reperfusion IIR Results For 6 weeks following there progressive increase serum creatinine with associated development albuminuria. increment above baseline determination...
Delayed graft function and primary non-function are serious complications following transplantation of kidneys derived from deceased brain dead (DBD) donors. α-melanocyte stimulating hormone (α-MSH) is a pleiotropic neuropeptide its renoprotective effects have been demonstrated in models acute kidney injury. We hypothesized that α-MSH treatment the recipient improves early reduces inflammation DBD transplantation. Eight Danish landrace pigs served as After four hours death both were removed...