A5101609323- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Wound Healing and Treatments
- Cervical Cancer and HPV Research
- RNA modifications and cancer
- Plant Disease Resistance and Genetics
- Proteoglycans and glycosaminoglycans research
- Electrospun Nanofibers in Biomedical Applications
- Connexins and lens biology
- Bioactive natural compounds
- Advanced biosensing and bioanalysis techniques
- Gut microbiota and health
- Cancer Genomics and Diagnostics
- Thermoregulation and physiological responses
- RNA Interference and Gene Delivery
- Fibroblast Growth Factor Research
- Plant and Fungal Interactions Research
- RNA Research and Splicing
- Cardiovascular Disease and Adiposity
- melanin and skin pigmentation
- Nanoplatforms for cancer theranostics
- Breast Cancer Treatment Studies
- Gene expression and cancer classification
- Cancer, Stress, Anesthesia, and Immune Response
Ningde Normal University
2025
First Affiliated Hospital Zhejiang University
2020-2024
Zhejiang University
2006-2024
Beihai People's Hospital
2020
Google (United States)
2018
Shaoxing People's Hospital
2017
Universidad Nacional Experimental Francisco de Miranda
2009
The development of multifunctional nanoformulations (NFs) include several features in a single nanosystem for these devices to overcome the disadvantages inefficiency and undesirable toxicity traditional therapies provide new opportunities management tumors. Herein, CaO2@Mn-PDA NFs with core-shell structure, integrating photothermal conversion properties Mn-PDA, chemodynamic doped Mn ions, relieving hypoxia tumor microenvironment (TME) were developed. as-fabricated embedded microneedles...
Tumor-associated macrophages (TAMs) are pivotal components of the breast cancer (BC) tumor microenvironment (TME), significantly influencing progression and response to therapy. However, heterogeneity specific roles TAM subpopulations in BC remain inadequately understood. We performed an integrated analysis single-cell RNA sequencing (scRNA-seq) bulk (RNA-seq) data from patients comprehensively characterize heterogeneity. Utilizing MetaTiME computational framework consensus clustering, we...
Abstract Background Matrine is a traditional Chinese medicine with significant inhibitory activity against malignant tumors. Its effects on the invasiveness and metastasis of tumors have rarely been reported. Aim To investigate whether matrine can inhibit metastasis‐related activities human melanoma cell line A375 in vitro . Methods 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay Annexin‐V–fluorescein isothiocyanate/propidium iodide (Annexin‐V‐FITC/PI) affinity were...
Aim: To prepare RGD-modified long circulating liposome (LCL) loading matrine (RGD-M-LCL) to improve the tumor-targeting and efficacy of matrine.Methods: LCL which was prepared with HSPC, cholesterol, DSPE-PEG2000 DSPE-PEG-MAL modified an RGD motif confirmed by high performance liquid chromatography (HPLC).The encapsulation efficiency RGD-M-LCL also detected HPLC.MTT assay used examine effects on proliferation Bcap-37, HT-29 A375 cells.The percentage apoptotic cells morphological changes in...
Breast cancer, which derives from the epithelium of mammary glands, is one most common cancers diagnosed in women globally.To date, authors many studies have reported that deregulation microRNAs (miRNAs) plays a crucial role occurrence, development, and metastasis tumors.Here, we discovered miR-660-5p was upregulated breast cancer cell lines MCF7 MDA-MB-231 compared with normal control line CCD-1095Sk.We then inhibited expression to investigate its biological function progression,...
Although microRNAs have been validated to play prominent roles in the occurrence and development of human bladder cancer (BC), alterations function many (miRNAs) invasion are not fully explored yet. miR-146b was reported be a tumor suppressor or oncomiRNA various types cancer. However, its accurate expression, function, mechanism remain unclear. Here we discovered that frequently upregulated tissues compared with adjacent non-cancerous tissues. Inhibition resulted significant inhibitory...
In this study, we propose an on-site electrochemical platform for sensitive simultaneous genotyping of the two major EGFR mutations (19del and L858R) through plasma ctDNA based on tetrahedral DNA nanostructure decorated screen-printed electrodes.
The expression profile of miRNAs and their function in condyloma acuminatum (CA) remains unknown. In this study, we aimed to detect the effects miR-143 miR-145, most downregulated CA samples using high-throughput sequencing, on cell proliferation apoptosis, determine a novel therapeutic target for recurrence. RT-qPCR was used validate lower miR-145 larger size samples, NRAS significantly higher than self-controls as determined by western blotting assay. Luciferase assay performed confirm...
Although the diversity and abundance of skin microbiome are mainly determined by intrinsic factors, including gender, age, anatomical site ethnicity, we question whether facial could be affected long-term exposure to airborne pollution. Using 16S rRNA gene amplicon sequencing, analyzed bacterial healthy young Chinese women (25~35-ys-old) between two districts with different AQI (air quality index) in Zhejiang Province. The overall structure was obviously these districts. It revealed an...
Aim: to investigate effects of Losartan on expression connexin 40 and 43 (Cx40 Cx43), in arteries at the early stage atherosclerosis a rabbit model.Methods: A total 28 male New Zealand white rabbits were divided into following groups: control group, high fat diet group (10 mg/kg/day).Losartan was administrated food for two weeks.Iliac obtained immunohistochemistry, transmission electron microscopy, Western blot, reverse transcriptase-polymerase chain reaction (RT-PCR).Results: Transmission...
Background Condyloma acuminatum (CA) is caused by low-risk human papillomavirus, and characterized high recurrence after treatment. The RNA modification N6-methyladenosine (m6A) plays an important role during diverse viral infections, including high-risk HPV infection in cervical cancer. However, it unclear whether changes the m6A methylation CA. Methods High-throughputm6A-sequencing was performed to profile transcriptome-wide mRNA modifications of CA tissues infected LR-HPVs paired normal...
Abstract Objective To identify differentially expressed long noncoding RNAs (lncRNAs) in condyloma acuminatum (CA) and to explore their probable regulatory mechanisms by establishing coexpression networks. Methods High-throughput RNA sequencing was performed assess genome-wide lncRNA expression CA paired adjacent mucosal tissue. The of candidate lncRNAs target genes larger specimens validated using real-time quantitative reverse transcriptase polymerase chain reaction (RT‒qPCR). Furthermore,...
Hydrogels possessing both conductive characteristics and notable self-healing to achieve high wound closure effectiveness is highly desirable in clinical applications. In this work, the stretchable hydrogel dressings were designed through dual-dynamic-bond cross-linking. A stable tricomplex molecule (PA@Fe) could be formed by pH-sensitive coordinate bond (catechol-Fe) between catechol groups PA ferric ions (Fe3+). And hydrogels with properties fabricated cross-linking of aldehyde PA@Fe...