A5101630255- Drug-Induced Hepatotoxicity and Protection
- Nephrotoxicity and Medicinal Plants
- Cancer Cells and Metastasis
- Mast cells and histamine
- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Retinal Development and Disorders
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Allergic Rhinitis and Sensitization
- Cancer Genomics and Diagnostics
- Animal Virus Infections Studies
- Metal complexes synthesis and properties
- Neural dynamics and brain function
- Hormonal Regulation and Hypertension
- DNA and Nucleic Acid Chemistry
- Heavy Metals in Plants
- Viral gastroenteritis research and epidemiology
- Asthma and respiratory diseases
- Receptor Mechanisms and Signaling
- EEG and Brain-Computer Interfaces
- Virus-based gene therapy research
- Functional Brain Connectivity Studies
- Respiratory Support and Mechanisms
- Metastasis and carcinoma case studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
Fujian Medical University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2017-2024
Fujian University of Traditional Chinese Medicine
2024
Chinese Academy of Agricultural Sciences
2023
Huazhong Agricultural University
2010-2020
Tianjin Medical University General Hospital
2011-2020
Medical College of Wisconsin
1990-2019
Tianjin Medical University
2011-2019
University of Michigan–Ann Arbor
2019
American Society of Anesthesiologists
2019
In cancer, epithelial-mesenchymal transition (EMT) is associated with metastasis. Characterizing EMT phenotypes in circulating tumor cells (CTCs) has been challenging because epithelial marker-based methods have typically used for the isolation and detection of CTCs from blood samples. The aim this study was to use optimized CanPatrol CTC enrichment technique classify using markers different types cancers. first step isolate via a filter-based method; then, an RNA situ hybridization...
// Ren Zhao 1, * , Zhen Cai 2, Sheng Li 3, Yong Cheng 4 Hua Gao 5 Fang Liu 6 Shiyang Wu Suyan Yan Dong Lei Zheng 2 Wenbin Zhang 7 Xiaojun 8 Xueqing Yao 3 1 Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School Medicine, Shanghai, China Laboratory Sino-UK Circulating Biomarkers' Exploration and Detection Center, Nanfang Southern Medical University, Guangzhou, 3Department surgery, Guangdong Hospital & Academy Sciences, Gastrointestinal First Affiliated Chongqing...
Em2007, a porcine reproductive and respiratory syndrome virus (PRRSV) variant with unique 68 aa deletion in Nsp2, was recently isolated China. Phylogenetic molecular evolutionary analyses indicated that Em2007 is natural recombinant between vaccine strain of PRRSV circulating virus. We also tested its pathogenicity piglets.
The rat CYP2D5 gene encodes a cytochrome P450 and is expressed in liver cells. Its expression commences few days after birth, maximal mRNA levels are achieved when animals reach puberty. Transfection DNA binding studies were performed to investigate the mechanism controlling developmentally programmed, liver-specific of CYP2D5. using series upstream chloramphenicol acetyltransferase fusion constructs identified segment between nucleotides -55 -156 that conferred transcriptional activity...
Enumeration and characterization of circulating tumor cells (CTCs) can provide information on patient prognosis treatment efficacy. However, CTCs are rare, making their isolation a major technological challenge. We developed technique for enrichment, subsequent based efficient depletion human leukocytes.The (CanPatrolTM CTC enrichment) we is red blood cell lysis to remove erythrocytes, followed by CD45+ leukocytes using magnetic bead separation method, virtue larger size, compared with...
The role of the trans-acting factor HNF-1α in activating CYP2E1 gene expression was confirmed by transient co-transfection an plasmid and promoter fused to chloramphenicol acetyl transferase (CAT) reporter gene. Only HNF-1α, not HNF-1β, HNF-4, C/EBPα, C/EBPβ, or DBP, able activate promoter. extent activation proportional number copies HNF-1 binding sequence upstream Removal mutation led inactivation response HNF-1α. Gel-shift Western blot analysis using a synthetic derived from rat liver...
In this study, we investigated the relationship between epithelial-mesenchymal transition phenotype of circulating tumor cells (CTCs) and distant metastasis in breast cancer patients. We analyzed expression epithelial (epithelial cell adhesion molecule, cytokeratin [CK]8, CK18 CK19) mesenchymal (vimentin TWIST1) markers CTCs from a large cohort Chinese patients (N=1083) using Canpatrol™ CTC assays. identified 84.9% (920/1083) enrolled study. Among these 920 patients, 547 showed CTCs, 793...
Background: Shuanghuanglian injection (SHLI) is a famous Chinese medicine used as an intravenous preparation for the treatment of acute respiratory tract infections. In recent years, immediate hypersensitivity reactions induced by SHLI have attracted broad attention. However, mechanism involved in these has not yet been elucidated. The present study aims to explore characteristics and deciphers role RhoA/ROCK signaling pathway reactions. Methods: SHLI-immunized mice or naive were...
Abstract During viral infection, the host cell synthesizes high amounts of proteins, which often causes stress to endoplasmic reticulum (ER). To manage abnormal ER stress, mammalian cells trigger a response called unfolded protein (UPR). Previous studies have indicated that porcine reproductive and respiratory syndrome virus (PRRSV), an Arterivirus has been devastating swine industry worldwide, can induce activate UPR, however, activation pathways biological significance requires further...
Cheqianzi Decoction (CQD) is a Traditional Chinese Medicine (TCM) formula comprising four herbs and recorded in the Ancient Materia Medica "Shengji Zonglu". Individually, these have been shown to reduce uric acid (UA) levels, treat hyperuricemia (HUA), alleviate kidney damage. However, therapeutic efficacy of CQD related mechanism are not yet clear. In this study, high performance liquid chromatography (HPLC) analysis confirmed that contents chemical components herbal medicines were...
Organoruthenium complexes containing 4-anilinoquinazolines show dual-targeting activity, and highly inhibit the growth of HeLa cancer cell line.
Asarum (Xixin), which contains analogues of aristolochic acid (AA), is the only species genus Aristolochia included in Chinese Pharmacopoeia 2020. However, contents and nephrotoxic effects AA analogs (Xixin) its formulations have not been clarified. An automatic, effective solid phase extraction process UPLC-MS/MS method were established for pretreatment quantitative detection commercially available traditional patent medicines. The cytotoxicity DNA damage induced by five evaluated CCK8...
RPE65 is essential for all-trans- to 11-cis-retinoid isomerization, the hallmark reaction of retinal pigment epithelium (RPE). Here, we identify regulatory elements in Rpe65 gene and demonstrate their functional relevance expression. We show that 5′ flanking region mouse gene, like human lacks a canonical TATA box consensus GC CAAT boxes. The genes do share several cis-acting elements, including an octamer, nuclear factor one (NFI) site, two E-box sites, suggesting conserved mode regulation....
Exoenzyme S of Pseudomonas aeruginosa (ExoS) is a member the family bacterial ADP-ribosylating exotoxins (bAREs). Site-directed mutagenesis glutamic acids within catalytic domain ExoS (termed ΔN222) allowed identification preferential inactivation ADP-ribosyltransferase activity by alanine substitution E381. The specific E381A mutant was 0.02% wild-type ΔN222. ΔN222(E381A) retained requirement factor activating exoenzyme (FAS) activation for expression activity. In contrast, E387A, E399A,...
Organometallic ruthenium(II) complexes [(η6-arene)Ru(en)Cl]+ (arene = e.g., biphenyl (1), dihydrophenanthrene, tetrahydroanthracene) show promising anticancer activity both in vitro and vivo are cytotoxic to cisplatin-resistant cancer cells, implying that these monofunctional have a different mechanism of action from bifunctional cisplatin. We demonstrate here complex 1 binds selectively the guanine base 15-mer single-stranded oligodeoxynucleotides (ODNs) 5′-CTCTCTX7G8Y9CTTCTC-3′ [X Y T; X...
Aims and objectives To test the effect of a Health Belief Model‐based nursing intervention on healthcare outcomes in Chinese patients with moderate to severe COPD . Background The H ealth B elief M odel ( HBM ) has been internationally validated variety chronic conditions. However, based is less explored Design A randomised controlled trial. Methods Enrolled were randomly assigned control groups. Patients group received 20‐ 30‐minute ‐based every 2 days during hospitalisation period after...