A5045345915- Advanced X-ray Imaging Techniques
- Advanced Electron Microscopy Techniques and Applications
- Hearing, Cochlea, Tinnitus, Genetics
- Digital Holography and Microscopy
- Vestibular and auditory disorders
- Neuroscience and Neural Engineering
- Nanopore and Nanochannel Transport Studies
- Force Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- Cryospheric studies and observations
- Advanced Fluorescence Microscopy Techniques
- Hearing Loss and Rehabilitation
- Nuclear Physics and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
University of Göttingen
2020-2025
Multi-scale X-ray phase contrast tomography (XPCT) enables three-dimensional (3D), non-destructive imaging of intact small animal cochlea and apical cochlear turns. Here we report on post-mortem excised non-human primate rodent cochleae at different [Formula: see text]-CT nano-CT synchrotron instruments. We explore sample embeddings, stainings regimes. Under optimized conditions preparation, instrumentation, protocol, retrieval, high image quality detail level can be achieved in 3D...
The cochlea of our auditory system is an intricate structure deeply embedded in the temporal bone. Compared with other sensory organs such as eye, has remained poorly accessible for investigation, example, by imaging. This limitation also concerns further development technology restoring hearing case cochlear dysfunction, which requires quantitative information on spatial dimensions and sensorineural status cochlea. Here, we employed X-ray phase-contrast tomography light-sheet fluorescence...
Improved hearing restoration by cochlear implants (CI) is expected optical (oCI) exciting optogenetically modified spiral ganglion neurons (SGNs) via an pulse generated outside the cochlea. The guided to SGNs inside cochlea flexible polymer-based waveguide probes. fabrication of these probes realized using 6" wafer-level micromachining processes, including lithography processes such as spin-coating cladding layers and a layer in between etch for structuring layer. Further adhesion metal...
We have used time-resolved small-angle X-ray scattering (SAXS) to study the adhesion of lipid vesicles in electrostatic strong-coupling regime induced by divalent ions. The bilayer structure and interbilayer distance dw between adhered was studied for different DOPC:DOPS mixtures varying surface charge density membrane, as well ions, such Ca2+, Sr2+, Zn2+. results are good agreement with strong coupling theory predicting state corresponding like-charge attraction based on ion-correlations....
Assessing the complex three-dimensional (3D) structure of cochlea is crucial to understanding fundamental aspects signal transduction in inner ear and a prerequisite for development novel cochlear implants. X-ray phase-contrast computed tomography offers destruction-free 3D imaging with little sample preparation, thus preserving delicate cochlea. The use heavy metal stains enables higher contrast resolution facilitates segmentation
Assessing the complex three-dimensional (3D) structure of cochlea is crucial to understand fundamental aspects signal transduction in inner ear and a prerequisite for development novel cochlear implants (CIs). Phase-contrast x-ray tomography (XPCT) offers destruction-free 3D imaging with little sample preparation, thus preserving delicate cochlea. In this work, we present XPCT data small-animal human cochleae obtained at dedicated synchrotron radiation (SR) inhouse µ-CT setups. For explore...
Altersbedingter Hörverlust ist die häufigste Form der Schallempfindungsschwerhörigkeit. Studien letzten Jahre an Nagetieren zeigen, dass altersbedingter hauptsächlich mit Synaptopathie von Bandsynapsen, dem Verlust inneren und äußeren Haarzellen (IHC, OHC) Spiralganglionneuronen (SGN) zusammenhängt.
Age-related hearing loss is the most common form of sensorineural loss. Studies from last years in rodents show that age-related mainly associated with synaptopathy ribbon synapses, inner and outer hair cells (IHC, OHC) spiral ganglion neurons (SGN).