The syntheses of a series novel ester-linked derivatives the icosahedral [closo-B12(OH)12]2- boron cluster (closomer esters) are described using several synthetic methods. reaction bis(tetrabutylammonium)-closo-dodecahydroxy-dodecaborate, [NBu4]21, with carboxylic acid chlorides and anhydrides, vinyl esters Y5(OiPr)13O catalyst 1,1'-carbonyldiimidazole-activated acids yields corresponding dianionic dodeca-ester closomers. method may be employed as general strategy. use elevated temperatures,...

Epithelial-msenchymal transition (EMT) contributes to posterior capsule opacification (PCO) type of cataract. Transcription factors Snail is a key trigger EMT activated by transforming growth factor β (TGF ). This study was done investigate the effect targeting siRNA on TGF 2-induced in human lens epithelial cells. 2 treatment cultured cell line (HLEB3) upregulated expression and relevant molecules such as vimentin α -SMA but downregulated keratin E-cadherin. After stimulation 2, HLEB3 cells...

Interleukin (IL)-17 has been implicated in a variety of inflammatory lung diseases. However, little is known about the expression and biological role IL‑17 acute injury (ALI). Therefore, aim present study was to confirm whether increase following ALI enhances cytokines/chemokines through activation extracellular signal‑regulated kinase (ERK)1/2 nuclear factor (NF)‑κB signaling pathway lipopolysaccharide (LPS)‑induced injury; which, turn, can be blocked by an antagonist. The authors indicated...

Multiple targets RNAi strategy is a preferred way to treat multigenic diseases, especially cancers. In the study, multi-target siRNAs were designed inhibit NET-1, EMS1 and VEGF genes in hepatocellular carcinoma (HCC) cells. And showed better silencing effects on VEGF, compared with single target siRNA. Moreover, siRNA greater suppression proliferation, migration, invasion, angiogenesis induced apoptosis HCC The results suggested that might be for cancer therapy could effective treatments.

Glycogen synthase kinase-3β (GSK-3β) is required in the expression of epithelial junction proteins.It was found downregulated hepatocellular carcinoma (HCC) tissues.The purpose this study to investigate role GSK-3β modulating metastatic behaviors human HCC cell lines vitro.In study, level measured 4 lines, and small interfering RNA (siRNA) vectors against or plasmids encoding were used evaluate responses target cells knockdown overexpression kinase, respectively.Our results showed that...

A recombinant phage display library was generated using splenocyte mRNA isolated from a Balb/c mouse hyperimmunized with hapten conjugate that mimicked the structure of methamidophos, one most acutely toxic organophosphate pesticides. Three single-chain variable fragment (scFv) antibodies highest specificity for termed 28D4, 29D0, and 36B2, were produced via stringent selection protocol. In competitive enzyme-linked immunosorbent assay, IC50 values 36B2 46.25, 35.39, 17.99 ng/mL,...

HAX-1 is an anti-apoptotic factor and overexpressed in many types of cancers.However, the functional role human cutaneous squamous cell carcinoma (cSCC) remains unclear.Our aim was to investigate expression cSCC its relationship with development cSCC.HAX-1 tissues in-vitro models were evaluated by real-time quantitative PCR (RT-qPCR), Western blot immunohistochemistry.And RNAi strategy used observe A431 cells.The mRNA protein level significantly higher compared normal tissue.There...

Purpose. This study was to determine the effect of CTGF-small interfering RNA (siRNA) on TGF- β 2-induced proliferation in human Tenon capsule fibroblasts (HTFs). Methods. HTFs were transfected with four CTGF-siRNAs separately for screening gene silencing efficacy that determined by transcript level measured quantitative real-time PCR (qRT-PCR). Recombinant 2 added into culture stimulate HTFs. The siRNAs evaluated qRT-PCR and immunofluorescence CTGF protein levels. viability cell counting...

The present study explored the expression of tetraspanin 1 (TSPAN1) in esophageal carcinoma (EC) and its association with clinicopathological factors. TSPAN1 small interfering RNA (siRNA) was designed to target gene Eca-109 cells order explore biological function proliferation apoptosis EC. results demonstrated that level EC tissue significantly increased compared adjacent normal (P<0.001). also associated histological differentiation, depth invasion, lymph node metastasis (all P<0.05) Ki-67...

This paper contains the experiment data on emodin, mice and cell survival rate, intestinal tissue H&E TUNEL staining, expression of p53 protein in small intestine, apoptosis, RNA vitro included. Data was worked out through MTT assay, Flow cytometry, Western blot, Real-time PCR Staining.

Abstract Background The purpose of this study is to explore the role and mechanism MMP-9 in EMT process thyroid cancer (TC), so as provide a basis for clinical exploration invasion metastasis TC, looking biological markers tumor molecular intervention therapy.Methods Western Blot RT-PCR were employed detect expression human normal cell line HT-ori3 TC lines IHH-4 (PTC), FTC-133 8505C. Expression levels EMT-related markers: epithelial marker E-cadherin stromal Vimentin TGF-1-induced detected...

Most patients with hepatocellular carcinoma (HCC) die of rapid progression and distant metastasis. Gene therapy represents a promising choice for HCC treatment, but the effective targeted methods are still limited.

RNA interference (RNAi) libraries screens have become widely used for small (sRNA) therapeutic targets development. However, conventional enzymatically libraries, typically prepared using the type 2 restriction enzyme MmeI, produce sRNAs between 18 and 20 bp, much shorter than usual lengths of 19-23 bp. Here we develop a size unbiased representative generated RNAi (SURER) library, which employs 3 modification EcoP15I to ranging from 19 23 bp group rationally designed linkers, can completely...

The occurrence and development of hepatocellular carcinoma (HCC) is a complicate process involved in genetic mutation epigenetic regulation. Successful HCC therapy needs multi-targets be involved. aim this study was to provide triple effective RNA (teRNA) which composed the specific siRNAs targeting NET-1 VEGF dsRNA activating TLR3, explored its anti-HCC roles mechanism. Real-time quantitative PCR (RT-qPCR), Western blot, immunofluorescence staining, MTT, Annexin V-FITC flow cytometry,...

RNA interference (RNAi) technology has been widely used in therapeutics development, especially multiple targeted RNAi strategy, which is a better method for gene suppression. In the study, interfering RNAs (iRNAs) were designed carrying two or three different siRNA sequences secondary structure formats (loop cloverleaf). By using these types of iRNAs, co-inhibition survivin and B-cell lymphoma-2 (Bcl-2) was investigated hepatocellular carcinoma (HCC) cells, we obtained promising silencing...