A5103253883- Molecular Biology Techniques and Applications
- Aquaculture disease management and microbiota
- Viral Infections and Immunology Research
- Vibrio bacteria research studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Liver Disease Diagnosis and Treatment
- Viral gastroenteritis research and epidemiology
- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
- Viral Infections and Vectors
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Mosquito-borne diseases and control
- Congenital gastrointestinal and neural anomalies
- Ubiquitin and proteasome pathways
- Digital Media Forensic Detection
- Adipokines, Inflammation, and Metabolic Diseases
- IL-33, ST2, and ILC Pathways
- MicroRNA in disease regulation
- Peptidase Inhibition and Analysis
- Salmonella and Campylobacter epidemiology
- Blood Coagulation and Thrombosis Mechanisms
- Ginseng Biological Effects and Applications
Guangzhou University of Chinese Medicine
2022-2024
Henan University
2024
People's Liberation Army 401 Hospital
2020-2024
Fujian Women and Children Hospital
2023
Fujian Medical University
2022
Shanghai Children's Medical Center
2022
Weifang Medical University
2020
Jackson and Tull (United States)
2020
Guangdong Medical College
2020
General Hospital of Jinan Military Command
2012-2018
MicroRNAs (miRNAs) play an essential role in breast malignant tumor development and progression. The of clinically validated biomarkers for primary cancer (BC) has remained insurmountable task despite other advances the field molecular biology. objective this study is to investigate differential expression miRNAs potential circulating microRNAs as novel biomarkers.Our analyses were performed on 48 tissue 100 serum samples patients with BC a set 20 control healthy women, respectively....
Inability to control autoimmunity is the primary barrier developing a cure for type 1 diabetes (T1D). Evidence that human cord blood-derived multipotent stem cells (CB-SCs) can autoimmune responses by altering regulatory T (Tregs) and islet β cell-specific cell clones offers promise new approach overcome underlying T1D. We developed procedure Stem Cell Educator therapy in which patient's blood circulated through closed-loop system separates lymphocytes from whole briefly co-cultures them...
To investigate the function of interleukin-33 (IL-33) in asthmatic airway remodeling and relationship between IL-33 asthma severity.IL-33 levels, sputum eosinophils percentage (EOS%), pulmonary total immunoglobulin (IgE) were measured for 45 patients with 40 non-allergic controls. Asthma severity was assessed. The expressions reticular basement membrane (RBM) on bronchial biopsy specimens from eight controls observed after hematoxylin-eosin staining (HE) immunohistochemical staining. In...
The prevalence of type 2 diabetes (T2D) is increasing worldwide and creating a significant burden on health systems, highlighting the need for development innovative therapeutic approaches to overcome immune dysfunction, which likely key factor in insulin resistance T2D. It suggests that modulation may be useful tool treating disease. In an open-label, phase 1/phase study, patients (N = 36) with long-standing T2D were divided into three groups (Group A, oral medications, n 18; Group B,...
Abstract A lasting imbalance between fatty acid synthesis and consumption leads to non-alcoholic liver disease (NAFLD), coupled with hepatitis insulin resistance. Yet the details of underlying mechanisms are not fully understood. Here, we unraveled that expression transcription factor Zbtb18 is markedly decreased in livers both patients murine models NAFLD. Hepatic knockout promoted NAFLD features like impaired energy expenditure oxidation (FAO), induced Conversely, hepatic overexpression...
Alcoholic liver disease (ALD) is a serious worldwide health problem. Ginsenoside Rc major active ingredient isolated from Panax ginseng, whose pharmacological effects counteract oxidative stress, inflammation, and lipid accumulation. However, it still unclear whether ginsenoside might exert beneficial on alcohol-induced injury. To this aim, mice primary hepatocytes (MPHs) were challenged with alcohol to test Rc's their intracellular metabolism. C57BL/6J or SIRT6alb-/- chronically fed diet...
Objectives: Farnesoid X receptor (FXR) activation is involved in ameliorating inflammatory bowel disease (IBD), such as ulcerative colitis (UC), and regulation may be its mechanism. Ginsenoside Rc (Rc) a major component of Panax ginseng, it plays an excellent role the anti-inflammatory processes. Our aim to explore alleviative effect on dextran sulfate sodium (DSS)-induced inflammation deficiencies barrier function based FXR signaling. Materials Methods:In vitro, we treated human intestinal...
The aim of the present study was to determine whether liraglutide (LRG), a long acting glucagon-like peptide 1 analogue, exerted protective effect on free fatty acid (FFA)‑treated pancreatic β‑cells via activating autophagy. INS‑1 insulinoma islet cell lines were treated with FFA and levels necrosis, apoptosis autophagy detected using an MTT assay, flow cytometry electron microscopy (ECM). A type 2 diabetes mellitus mouse model established through treatment mice high‑fat diet for 8 weeks...
Abstract Background Microglia play a pivotal role in neuroinflammation, while obesity triggers hypothalamic microglia activation and inflammation. Sirt6 is an important regulator of energy metabolism many peripheral tissues anorexic neurons. However, the exact mechanism for controlling high-fat diet-induced remain unknown. Methods expression levels under various nutritional conditions were measured hypothalamus mice. Also, Sirt6-deficient mice provided diets to monitor metabolic changes...
BACKGROUND Papillary thyroid cancer (PTC) is currently the most commonly diagnosed endocrine malignancy. In addition, sex- and age-adjusted incidence of PTC has exhibited a greater increase over last 2 decades than in many other malignancies. Thus, discovering noninvasive specific serum biomarker to distinguish from cancer-free controls its early stages remains an important goal. MATERIAL AND METHODS Serum samples 88 patients 80 were randomly allocated into training or validation sets....
Vibrio alginolyticus is a Gram-negative halophilic bacterium and has been recognized as an opportunistic pathogen in both humans marine animals. It the causative agent of food-borne diseases, such gastroenteritis, it invades through wounds predisposed individuals. In this study, we present completed genome V. ATCC 17749(T) high-throughput sequencing.
Nonalcoholic fatty liver disease (NAFLD) is a prevalent global condition and common precursor to cancer, yet there currently no specific medication available for its treatment. Ginseng, renowned medicinal dietary properties, has been utilized in NAFLD management, although the precise underlying mechanism remains elusive. To investigate effectiveness of ginsenoside Rd, we employed mouse cell models induce using high-fat diets, oleic acid, palmitic acid. We explored confirmed Rd-induced...
Acetaminophen (APAP) intake leads to excessive NAPQI deposition, stimulating inflammatory and oxidative stress causing fatal liver injury. However, the detailed molecular mechanism involved is unknown, effective therapeutic approaches remain insufficient. In this study, we discovered that treatment with ginsenoside Rc can prevent response caused by APAP in mouse primary hepatocytes (MPHs), along corresponding changes related genes. Additionally, Ginsenoside effectively alleviates...
Summary 1. UbcH10 is the cancer‐related E2 ubiquitin‐conjugating enzyme, and its overexpression has been demonstrated in a variety of malignancies. The aim present study to silence gene by RNA interference (RNAi) observe inhibitory effect on colorectal cancer cell growth vitro vivo . 2. We constructed expression vector pGPU6/GFP/Neo/UbcH10‐RNAi (pUbcH10‐RNAi), which contained short hairpin cassette. Then silencing lines LoVo/UbcH10‐RNAi HT‐29/UbcH10‐RNAi were established. Reverse...
Vibrio vulnificus, V. alginolyticus, and parahaemolyticus are commonly opportunistically pathogenic to humans.In this study, a novel multiple touchdown polymerase chain reaction method (MT-PCR) was developed benefit rapid simultaneous detection of the presence three species from enriched clinical environmental samples.The showed sensitivity 104 colony forming units (CFU)/mL for 103 CFU/mL specificity 100% all species. All strains were detected in spiked samples artificially contaminated with...
Objective To determine the prevalence and distribution of human enteroviruses (HEVs) among healthy children in Shenzhen, China. Method Clinical specimens were obtained from 320 under 5 years old China 2010 to 2011. The evaluated using real-time PCR cell cultures. positive further tested reverse transcription-seminested (RT-snPCR). Molecular typing phylogenetic analysis based on sequence determined. Results Among samples, 34 for HEVs (10.6%) 22 different serotypes identified RT-snPCR. PV1 PV2...
Aims. Noncoding RNAs (ncRNAs) play an important role in the occurrence and development of type 2 diabetes mellitus (T2DM). This paper summarized current evidences involvement microRNAs, long noncoding (lncRNAs), circular (circRNAs) differential expressions their interaction with each other T2DM. Methods. The differentially expressed miRNAs, lncRNAs, circRNAs blood circulation (plasma, serum, whole blood, peripheral mononuclear cells) patients T2DM were found PubMed, GCBI, databases....