A5049051399- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Tissue Engineering and Regenerative Medicine
- Angiogenesis and VEGF in Cancer
- Periodontal Regeneration and Treatments
- Cancer Cells and Metastasis
- Reproductive System and Pregnancy
- Organ and Tissue Transplantation Research
- Sarcoma Diagnosis and Treatment
- Bone Tissue Engineering Materials
- Immune cells in cancer
- Cell Adhesion Molecules Research
- Hematological disorders and diagnostics
- Bone fractures and treatments
- Multiple Myeloma Research and Treatments
- Adipose Tissue and Metabolism
- Wound Healing and Treatments
- RNA Interference and Gene Delivery
- Acute Myocardial Infarction Research
- Coronary Interventions and Diagnostics
- Neuroinflammation and Neurodegeneration Mechanisms
- Proteoglycans and glycosaminoglycans research
- Inflammation biomarkers and pathways
- Electrospun Nanofibers in Biomedical Applications
- Hedgehog Signaling Pathway Studies
Inserm
2014-2024
École Nationale Vétérinaire de Toulouse
2020-2024
Centre National de la Recherche Scientifique
2014-2024
Université de Toulouse
2016-2023
Université Toulouse III - Paul Sabatier
2011-2023
RESTORE
2023
Établissement Français du Sang
2007-2020
Hôpital Purpan
2019
StromaLab
2013-2017
Centre National pour la Recherche Scientifique et Technique (CNRST)
2013
Abstract Adult bone marrow-derived mesenchymal stem cells (MSCs) are multipotent that the subject of intense investigation in regenerative medicine. In addition, MSCs possess immunomodulatory properties with therapeutic potential to prevent graft-versus-host disease (GvHD) allogeneic hematopoietic cell transplantation. Indeed, can inhibit natural killer (NK) function, modulate dendritic maturation, and suppress T-cell response. Here, we report nonclassic human leukocyte antigen (HLA) class I...
Adult bone marrow-derived mesenchymal stem cells (MSCs) are multipotential capable of regenerating injured tissues. In addition to their multipotency, MSCs inhibit natural killer cell cytotoxicity and T-lymphocyte alloproliferation. Several immunosuppressive mechanisms have been described, including indoleamine 2, 3, -dioxygenase-induced depletion tryptophan from the lymphocyte environment, secretion prostaglandin E2 other factors. Here, we review data supporting a new MSC immunoregulation...
Abstract Bone marrow mesenchymal stem cells ( MSC s) are plastic adherent that can differentiate into various tissue lineages, including osteoblasts, adipocytes and chondrocytes. However, this progenitor property is not shared by all within the population. In addition, s vary in their proliferation capacity expression of markers. Because heterogeneity CD 146 population, we compared −/Low High under clonal conditions after sorting non‐clonal cell population to determine whether associated...
Native human subcutaneous adipose tissue (AT) is well organized into unilocular adipocytes interspersed within dense vascularization. This structure completely lost under standard culture conditions and may impair the comparison with native tissue. Here, we developed a 3-D model of white AT reminiscent cellular architecture found in vivo. Starting progenitors derived from stromal-vascular fraction AT, generated spheroids which endogenous endothelial cells self-assembled to form highly...
Human bone marrow mesenchymal stem cells (MSC) generate, via a fibroblast colony-forming unit (CFU-F), osteo-chondroblastic as well adipocytes and stromacytes. To date, these are isolated indirectly using cell culture method phenotyped CD45 negative while the in vivo counterparts undetermined. Our aim was to develop direct selection determine phenotype of MSC this way. Mesenchymal were selected with anti-CD49a and/or anti-CD45 antibodies either flow cytometry or magnetic beads method. All...
Background Osteosarcoma is the most common malignant primary bone tumour in young adult treated by neo adjuvant chemotherapy, surgical tumor removal and multidrug chemotherapy. For correction of soft tissue defect consecutive to surgery and/or treatment, autologous fat graft has been proposed plastic reconstructive surgery. Principal Findings We report here a case late local recurrence osteosarcoma which occurred 13 years after initial pathology 18 months lipofilling procedure. Because such...
Abstract The possibility of using adipose tissue-derived stromal cells (ATSC) as alternatives to bone marrow-derived (BMSC) for repair has garnered interest due the accessibility, high cell yield, and rapid in vitro expansion ATSC. For clinical relevance, their forming potential comparison BMSC must be proven. Distinct differences between ATSC have been observed osteogenic vivo is not clear date. aim current study was compare osteogenesis human xenofree-expanded an ectopic nude mouse model...
RNA delivery is an attractive strategy to achieve transient gene expression in research projects and cell- or gene-based therapies. Despite significant efforts investigating vector-directed transfer, there still a requirement for better efficiency of primary cells vivo. Retroviral platforms drive delivery, yet retrovirus RNA-packaging constraints limit transfer two genome-molecules per viral particle. To improve retroviral we designed dimerization-independent MS2-driven packaging system...
Mesenchymal stem cells (MSCs) are multipotent that can be obtained from several sources such as bone marrow and adipose tissue. Depending on the culture conditions, they differentiate into osteoblasts, chondroblasts, adipocytes, or neurons. In this regard, constitute promising candidates for cell-based therapy aimed at repairing damaged tissues. addition, MSCs display immunomodulatory properties through expression of soluble factors including HLA-G. We here analyse both immunogenicity...
Mesenchymal stem cells (MSCs) and pericyte progenitors (PPs) are both perivascular with similar multipotential properties regardless of tissue origin. We compared the phenotype function 2 cell types derived from same bone-marrow samples but expanded in their respective media – conditions (endothelial growth medium [EGM-2]) for PPs standard (mesenchymal [MSM]) MSCs. After 3 weeks culture, whatever expansion medium, all showed characteristics (MSC markers adipo-osteo-chondroblastic...
The biological effects of indium-tin-oxide (ITO) are considerable importance because workers exposed to indium compounds have been diagnosed with interstitial lung disease or pulmonary alveolar proteinosis; however, the pathophysiology these diseases is undefined. Here, mice intraperitoneally inoculated ITO-nanoparticles (ITO-NPs) resulted in peritonitis dependent NLRP3 inflammasome, neutrophils recruitment and interleukin-1β (IL-1β) production. Withal peritoneal macrophages ex vivo ITO-NPs...
Abstract Similar to other adult tissue stem/progenitor cells, bone marrow mesenchymal stem/stromal cells ( BM MSC s) exhibit heterogeneity at the phenotypic level and in terms of proliferation differentiation potential. In this study such a was reflected by CD 200 protein. We thus characterized pos sorted from whole cultures we investigated molecular mechanisms regulating expression. After sorting, measurement lineage markers showed that osteoblastic genes RUNX 2 DLX 5 were up‐regulated...
Abstract Obesity and type 2 diabetes are becoming a global sociobiomedical burden. Beige adipocytes emerging as key inducible actors putative relevant therapeutic targets for improving metabolic health. However, in vitro models of human beige adipose tissue currently lacking hinder research into this cell biotherapy development. Unlike traditional bottom‐up engineering approaches that aim to generate building blocks, here scalable system is proposed pre‐vascularized functional organoids...
In this work we studied the expression of adhesion molecules on primate human and non-human marrow stromal cells (primary cultures lines) CD34+ hematopoietic normal leukemic precursors. Differential α1 integrin subunit was observed, since molecule intensely expressed by stroma but not detected cells. We used difference to select, in fresh bone samples, α1-positive found that all precursors giving rise colony-forming units-fibroblasts (CFU-F) were present fraction. No colonies α1-negative...