A5100647133- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Microbial infections and disease research
- Plant Pathogenic Bacteria Studies
- Bacterial Infections and Vaccines
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Tea Polyphenols and Effects
- Genomics and Phylogenetic Studies
- Atmospheric chemistry and aerosols
- Infections and bacterial resistance
- Air Quality and Health Impacts
- Food Quality and Safety Studies
- Antimicrobial Resistance in Staphylococcus
- Pneumonia and Respiratory Infections
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Toxin Mechanisms and Immunotoxins
- Milk Quality and Mastitis in Dairy Cows
- vaccines and immunoinformatics approaches
- RNA and protein synthesis mechanisms
- Clostridium difficile and Clostridium perfringens research
- Solid State Laser Technologies
- Forest ecology and management
- Air Quality Monitoring and Forecasting
Nanjing Agricultural University
2013-2025
Anhui Agricultural University
2014-2024
Jinan University
2015-2024
Guangdong-Hongkong-Macau Joint Laboratory of Collaborative Innovation for Environmental Quality
2024
Sanya University
2023-2024
Experimental Center of Tropical Forestry
2023
Chinese Academy of Forestry
2023
Research Institute of Tropical Forestry
2023
Zhengzhou University
2022-2023
China University of Geosciences (Beijing)
2023
A total of 98 compounds including 20 aldehydes, eight arenes, six acids, 17 alcohols, 13 ketones, nine esters, methoxyphenolics, three alkenes, seven alkanes, and other components were tentatively identified in Chinese dark teas (CDTs) using gas chromatography-mass spectrometry. Multivariate statistical analysis revealed that from Yunnan Guangxi provinces could be classified into one group, CDTs belonged to the cluster. The diagnostic volatile being responsible for CDTs' discrimination...
Streptococcus suis (S. suis) is a zoonotic pathogen with multiple serotypes, and thus, multivalent vaccines generating cross-protection against S. infections are urgently needed to improve animal welfare reduce antibiotic abuse. In this study, we established systematic comprehensive epitope prediction pipeline based on immunoinformatics. Ten candidate epitopes were ultimately selected for building the multi-epitope vaccine (MVSS) infections. The ten of MVSS all derived from highly conserved,...
Novel
Tea is one of the most consumed beverages worldwide. The healthy effects tea are attributed to a wealthy different chemical components from tea. Thousands studies on constituents had been reported. However, data these individual reports have not collected into single database. lack curated database related information limits research in this field, and thus cohesive system should necessarily be constructed for deposit further application.The Metabolome (TMDB), manually web-accessible...
Abstract Background Streptococcus agalactiae , also referred to as Group B (GBS), is a frequent resident of the rectovaginal tract in humans, and major cause neonatal infection. In addition, S. known fish pathogen, which compromises food safety represents zoonotic hazard. The complete genome sequence piscine isolate GD201008-001 was compared with 14 other piscine, human bovine strains explore their virulence determinants, evolutionary relationships genetic basis host tropism . Results...
Streptococcus agalactiae is the causative agent of septicemia and meningitis in fish. Previous studies have shown that hyaluronidase (Hyl) an important virulence factor many Gram-positive bacteria. To investigate role S. Hyl during interaction with macrophages, we inactivated gene encoding extracellular hyaluronidase, hylB, a clinical Hyl(+) isolate. The isogenic hylb mutant (Δhylb) displayed reduced survival macrophages compared to wild type stimulated significantly higher release...
Streptococcus agalactiae (Group B Streptococcus, GBS) is a multi-host pathogen, even causing life-threatening infections in newborns. Vaccination with GBS crossed serotypes vaccine one of the best options for long-term infection control. Here we built comprehensive silico epitope-prediction workflow pipeline to design multivalent multiepitope-based subunit containing 11 epitopes against (MVSA). All MVSA came from proteins which were antigenic-confirmed, virulent-associated, surface-exposed...
Prophages play important roles in the transduction of various functional traits, including virulence factors, but remain debatable harboring and transmitting antimicrobial resistance genes (ARGs). Herein we characterize a prevalent family prophages Streptococcus, designated SMphages, which harbor twenty-five ARGs that collectively confer to ten classes, vanG-type vancomycin locus oxazolidinone gene optrA. SMphages integrate into four chromosome attachment sites by utilizing three types...
Cellular senescence is linked to aging and tumorigenesis. The of mesenchymal stem cells (MSCs) may influence the tumor growth, metastasis, angiogenesis by secreting a variety cytokines growth factors. conditioned media adipose derived MSCs (AD-MSCs) stimulated proliferation human LoVo colorectal-cancer cells, replicative senescent had more obvious effects in comparison that premature AD-MSCs. Analysis factors secreted culture determined expressed high levels galectin-3. Galectin-3 expression...
Streptococcus suis (SS) is a zoonotic pathogen that causes severe disease symptoms in pigs and humans. Biofilms of SS bind to extracellular matrix proteins both endothelial epithelial cells cause persistent infections. In this study, the differences protein expression profiles grown either as planktonic or biofilms were identified using comparative proteomic analysis. The results revealed existence 13 varying amounts, among which six upregulated seven downregulated biofilm compared with...
The binding of transcription factors (TFs) to TF sites plays a vital role in the process regulating gene expression and evolution. With development machine learning deep learning, some successes have been achieved predicting sites. In this paper, we develop model, BTFBS, which predicts whether bacterial combine or not. model takes both amino acid sequences nucleotide as inputs, extracts features through convolutional neural network MultiheadAttention. For use two negative sample sampling...
ABSTRACT This work describes a whole-genome sequence of Streptococcus agalactiae strain GD201008-001, pathogen causing meningoencephalitis in cultural tilapia China. The genome provides opportunities to understand the piscine GBS pathogenicity and its genetic basis associated with host tropism.
Swine extraintestinal pathogenic Escherichia coli (ExPEC) is an important pathogen that leads to economic and welfare costs in the swine industry worldwide, occurring with increasing frequency China. By far, various virulence factors have been recognized ExPEC. Here, we investigated genotypes clonal structure of collected strains improve knowledge phylogenetic traits porcine ExPECs China.We isolated 64 Chinese ExPEC from 2013 14 multiplex PCR, distribution isolates belonging groups B1, B2, A...
Clustered regularly interspaced palindromic repeats (CRISPR) and their associated cas genes have been demonstrated to regulate self-genes virulence in many pathogens. In this study, we found that inactivation of cas9 caused reduced adhesion intracellular survival the piscine Streptococcus agalactiae strain GD201008-001 significantly decreased zebrafish mice. Further investigation indicated regR transcriptional regulator was upregulated Δcas9 mutant. As mediates repression hyaluronidase, a...
The clustered regularly interspaced palindromic repeat (CRISPR)-associated (Cas) system functions classically as a prokaryotic defense against invading mobile genetic elements, such phages, plasmids, and viruses. Our previous study revealed that CRISPR deletion caused increased transcription of capsular polysaccharide (CPS) synthesis-related genes severely attenuated virulence in the hypervirulent piscine Streptococcus agalactiae strain GD201008-001. Here, we found deficiency resulted...
Streptococcus suis serotype 2 (SS2) is a zoonotic pathogen that can cause infections in pigs and humans. Bacterial surface proteins are often investigated as potential vaccine candidates biomarkers of virulence. In this study, novel method for identifying bacterial presented, which combines immunoproteomic immunoserologic techniques. Critical to the success new an improved procedure generating two-dimensional electrophoresis gel profiles S. proteins. The identified study include...
Streptococcus agalactiae, long recognized as a mammalian pathogen, is an emerging concern with regard to fish. In this study, we used mouse model and in vitro cell infection evaluate the pathogenetic characteristics of S. agalactiae GD201008-001, isolated from tilapia China. This bacterium was found be highly virulent capable inducing brain damage by migrating into crossing blood–brain barrier (BBB). The phagocytosis assays indicated that could internalized murine macrophages survive...
Streptococcus suis is a prominent pathogen causing septicemia and meningitis in swine humans. Bacitracin used widely as growth promoter animal feed to control the spread of necrotic enteritis most developing countries. This study aimed characterize novel membrane transporter module Sst comprising SstE, SstF, SstG for bacitracin resistance.Comparative genomics protein homology analysis found potential efflux pump SstFEG encoded upstream well-known bacitracin-resistance genes bceAB bceRS. A...
Six D-π-A model compounds (compounds 1-6) were conveniently synthesized and characterized by 1H NMR, 13C MS single crystal X-ray diffraction. One photon absorption emission properties studied using a series of UV-visible fluorescence spectra theoretical calculations applied to investigate the structure-property relationships, which showed that all six possessed an obvious intramolecular charge transfer process could be attributed their optical properties. We simultaneously investigated...