A5067923572- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Plant Pathogenic Bacteria Studies
- Microbial infections and disease research
- Viral gastroenteritis research and epidemiology
- Antimicrobial Resistance in Staphylococcus
- Animal Virus Infections Studies
- Escherichia coli research studies
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Infections and bacterial resistance
- Genomics and Phylogenetic Studies
- Infective Endocarditis Diagnosis and Management
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Viral Infections and Immunology Research
- Toxin Mechanisms and Immunotoxins
- Bacterial Genetics and Biotechnology
- Animal Disease Management and Epidemiology
- Salmonella and Campylobacter epidemiology
- Aquaculture disease management and microbiota
- Herpesvirus Infections and Treatments
- Bacterial Infections and Vaccines
- Plant Virus Research Studies
- Biochemical and Structural Characterization
Nanjing Agricultural University
2016-2025
GTx (United States)
2019
Ministry of Agriculture and Agro Based Industry
2018
Ministère de l'Agriculture et de la Souveraineté alimentaire
2018
Ministry of Agriculture & Farmers Welfare
2018
Huzhou Central Hospital
2015
Ludwig-Maximilians-Universität München
1991
Abstract Outbreaks in fish of motile Aeromonad septicemia (MAS) caused by Aeromonas hydrophila have a great concern worldwide. Here, for the first time, we provide two complete genomes epidemic A. strains isolated China. To gain an insight into pathogenicity , performed comparative genomic analyses five belonging to sequence type (ST) 251, together with environmental strain ATCC 7966 T . We found that known virulence factors, including III secretion system, VI system and lateral flagella,...
The type VI secretion system (T6SS) is a widespread molecular weapon deployed by many bacterial species to target eukaryotic host cells or rival bacteria. Using dynamic injection mechanism, diverse effectors can be delivered T6SS directly into recipient cells. Here, we report new family of encoded extended Hcps carrying toxin domains. Bioinformatic analyses revealed that these with C-terminal extension toxins, designated as Hcp-ET, exist widely in the Enterobacteriaceae. To verify our...
Porcine epidemic diarrhea has become pandemic in the Asian pig-breeding industry, causing significant economic loss. In present study, 11 complete genomes of porcine virus (PEDV) field isolates from China were determined and analyzed. Frequently occurring mutations observed, which suggested that full understanding genomic epidemiological characteristics is critical fight against PEDV epidemics. Comparative analysis 49 available clustered strains into (PX) classical (CX) groups identified...
Summary The type VI secretion system (T6SS) of bacteria plays a key role in competing for specific niches by the contact‐dependent killing competitors. Recently, Rhs proteins with polymorphic C‐terminal toxin‐domains that inhibit or kill neighboring cells were identified. In this report, we identified novel an MPTase4 (Metallopeptidase‐4) domain (designated as Rhs‐CT1) showed antibacterial effect via T6SS Escherichia coli . We managed to develop strategy matching diagnostic...
Streptococcus suis (S. suis) is a zoonotic pathogen with multiple serotypes, and thus, multivalent vaccines generating cross-protection against S. infections are urgently needed to improve animal welfare reduce antibiotic abuse. In this study, we established systematic comprehensive epitope prediction pipeline based on immunoinformatics. Ten candidate epitopes were ultimately selected for building the multi-epitope vaccine (MVSS) infections. The ten of MVSS all derived from highly conserved,...
ABSTRACT Streptococcus suis is an emerging zoonotic pathogen causing severe infections in pigs and humans. In previous studies, 33 serotypes of S. have been identified using serum agglutination. Here, we describe a novel strain, CZ130302, isolated from outbreak acute piglet meningitis eastern China. Strong pathogenicity caused by strain CZ130302 was reproduced the BALB/c mouse model. The showed high fatality rate (8/10), higher than those for known virulent serotype 2 strains P1/7 (1/10)...
Type VI secretion systems (T6SSs) are involved in the pathogenicity of several Gram-negative bacteria. The VgrG protein, a core component and effector T6SS, has been demonstrated to perform diverse functions. N-terminal domain protein is homologue tail fiber gp27 phage T4, which performs receptor binding function determines host specificity. Based on sequence analysis, we found that two putative T6SS loci exist genome avian pathogenic Escherichia coli (APEC) strain TW-XM. To assess...
Streptococcus suis (SS) is an important pathogen of pigs, and it also recognized as a zoonotic agent for humans. SS infection may result in septicemia or meningitis the host. However, little known about genes that contribute to virulence process survival within host blood cerebrospinal fluid (CSF). Small RNAs (sRNA) have emerged key regulators several bacteria, but they not been investigated SS. Here, using differential RNA-sequencing approach from strain P1/7 grown rich medium, pig blood,...
Bacterial cell wall (CW) and extracellular (EC) proteins are often involved in interactions with matrix (ECM) such as laminin (LN) fibronectin (FN), which play important roles adhesion invasion. In this study, an efficient method combining proteomic analysis Far-Western blotting assays was developed to screen directly for bacterial surface LN- FN-binding capacity. With approach, fifteen potential LN-binding five were identified from Streptococcus suis serotype 2 (SS2) CW EC proteins. Nine...
Streptococcus suis is a major bacterial pathogen in pigs and an emerging zoonotic pathogen. Different S. serotypes exhibit diverse characteristics population structure pathogenicity. Surveillance data highlight the significance of serotype 4 (SS4) swine streptococcusis, pathotype causing human infections. However, except for few epidemiologic studies, information on SS4 remains limited. In this study, we investigated structure, pathogenicity, antimicrobial based 126 isolates, including one...
ABSTRACT The use of phages as antibacterial agents is limited by their generally narrow host ranges. aim this study was to make a T4-like phage, WG01, obtain the range another QL01, replacing its host-determinant gene region with that QL01. This process triggered direct expansion WG01 range. offspring obtained ranges both QL01 and well ability infect eight additional bacteria in comparison wild-type strains. WQD had widest range; therefore, corresponding fragments, named QD, could be used...
Streptococcus agalactiae (Group B Streptococcus, GBS) is a multi-host pathogen, even causing life-threatening infections in newborns. Vaccination with GBS crossed serotypes vaccine one of the best options for long-term infection control. Here we built comprehensive silico epitope-prediction workflow pipeline to design multivalent multiepitope-based subunit containing 11 epitopes against (MVSA). All MVSA came from proteins which were antigenic-confirmed, virulent-associated, surface-exposed...
Bovine coronavirus (BCoV) is a major cause of infectious disease in cattle, causing huge economic losses to the beef and dairy industries worldwide. BCoV can infect humans multiple other species animals. A rapid, reliable, simple test needed detect infection suspected farms. In this study, we developed novel multienzyme isothermal rapid amplification (MIRA) lateral flow dipstick (LFD) combination assay, targeting highly conserved region viral nucleocapsid (N) gene for detection. The MIRA-LFD...
Numerous bacterial pathogens have evolved tactics to interfere with the host ubiquitination network evade clearance by innate immune system. Nevertheless, subtle antagonism between a ubiquitinase and deubiquitinase, through which they modify their respective targets within multifaceted network, has yet be characterized. BRCC3 isopeptidase complex (BRISC) is newly identified K63-specific deubiquitinase that plays crucial role in cellular signaling pathways such as inflammation. NleG, type III...
Muramidase-released protein (MRP) of Streptococcus suis serotype 2 (SS2) is an important epidemic virulence marker with unclear role in bacterial infection. To investigate the biologic functions MRP, 3 mutants named Δmrp, Δmrp domain 1 (Δmrp-d1), and (Δmrp-d2) were constructed to assess phenotypic changes between parental strain mutant strains. The results indicated that MRP (MRP-D1, non-conserved region from a virulent strain, a.a. 242–596) played critical adherence SS2 host cells, compared...
Swine extraintestinal pathogenic Escherichia coli (ExPEC) is an important pathogen that leads to economic and welfare costs in the swine industry worldwide, occurring with increasing frequency China. By far, various virulence factors have been recognized ExPEC. Here, we investigated genotypes clonal structure of collected strains improve knowledge phylogenetic traits porcine ExPECs China.We isolated 64 Chinese ExPEC from 2013 14 multiplex PCR, distribution isolates belonging groups B1, B2, A...