A5015154794- Pluripotent Stem Cells Research
- Telomeres, Telomerase, and Senescence
- Renal and related cancers
- DNA Repair Mechanisms
- Retinoids in leukemia and cellular processes
- Antioxidant Activity and Oxidative Stress
- MicroRNA in disease regulation
- Genetic Syndromes and Imprinting
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Glutathione Transferases and Polymorphisms
- Angiogenesis and VEGF in Cancer
- Cancer Cells and Metastasis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cancer Immunotherapy and Biomarkers
- CRISPR and Genetic Engineering
- Cancer, Lipids, and Metabolism
- Spine and Intervertebral Disc Pathology
- Circular RNAs in diseases
- Electrodeposition and Electroless Coatings
- Autophagy in Disease and Therapy
- Psoriasis: Treatment and Pathogenesis
- Salivary Gland Disorders and Functions
- Molecular Biology Techniques and Applications
Nanjing University
2025
Indiana University – Purdue University Indianapolis
2024
Newcastle University
2011-2023
Tianjin Medical University General Hospital
2016-2023
Salk Institute for Biological Studies
2021-2022
North Sichuan Medical University
2021
People’s Hospital of Rizhao
2018
Nantong University
2018
Tianjin Medical University
2016
Kansas State University
2004-2015
Short-term, systemic expression of the Yamanaka reprogramming factors (Oct-3/4, Sox2, Klf4 and c-Myc [OSKM]) has been shown to rejuvenate aging cells promote tissue regeneration in vivo. However, mechanisms by which OSKM promotes are unknown. In this work, we focus on a specific demonstrate that local OSKM, specifically myofibers, induces activation muscle stem or satellite (SCs), accelerates young mice. contrast, expressing directly SCs does not improve regeneration. Mechanistically,...
Mammals have limited regenerative capacity, whereas some vertebrates, like fish and salamanders, are able to regenerate their organs efficiently. The regeneration in these species depends on cell dedifferentiation followed by proliferation. We generate a mouse model that enables the inducible expression of four Yamanaka factors (Oct-3/4, Sox2, Klf4, c-Myc, or 4F) specifically hepatocytes. Transient vivo 4F induces partial reprogramming adult hepatocytes progenitor state concomitantly...
Background Our previous study demonstrated that CCL26 secreted by cancer-associated fibroblasts (CAF) promoted the invasive phenotype of oral squamous cell carcinoma (OSCC), however, more comprehensive clinical expression patterns and its role in immunotherapy remains ambiguous. Methods levels different cancer normal tissues were analyzed validated 67 OSCC patients through immunohistochemical staining (IHC). The spatial distribution pattern tumor microenvironment was determined, outcomes...
Cells accumulate single-stranded DNA (ssDNA) when telomere capping, replication, or repair is impeded. This accumulation leads to cell cycle arrest through activating the DNA–damage checkpoints involved in cancer protection. Hence, ssDNA could be an anti-cancer mechanism. However, has above a certain threshold activate checkpoints. What determines this checkpoint-activation important, yet unanswered question. Here we identify Rif1 (Rap1-Interacting Factor 1) as threshold-setter. Following...
During bent bone grinding, the robot often needs to adjust its grinding depth and angle in real time ensure surgical quality. Optical- or vision-based navigation methods are no longer applicable due interference caused by splashing cooling water operating field. Inspired orthopedic surgeons, this article, we present a tactile perception-based method solve problem. The effect of parameters on cutter's current vibration is analyzed, signals further processed predict angle. Depth estimation...
MicroRNAs (miRNAs) are endogenous non-coding RNAs of 19-25 nucleotides in length. Abnormal miRNA expression has been identified various types cancer. However, the aberrant miR-1244 not reported acute myeloid leukemia.First, miR-144-3p was explored bone marrow and peripheral blood AML patients healthy control. Then, we also evaluated level HL-60 cells. The possible target gene predicted using TargetScan. Dual luciferase reporter assay applied to validate miR-144-3p. Cell viability apoptosis...
Type 2 diabetes (T2D) is on the rise worldwide and associated with various complications of oral cavity. Using an adult-onset preclinical model, we demonstrated profound periodontal alterations in T2D mice, including inflamed gingiva, disintegrated ligaments (PDL), marked alveolar bone loss, unbalanced remodeling due to decreased formation increased resorption. Notably, observed elevated levels Wnt signaling inhibitor sclerostin mice. Motivated by these findings, investigated whether a...
The objective of the present research work was to evaluate anticancer properties luteolin against SH-SY5Y neuroblastoma tumor cell line. Cell viability evaluated by MTT assay after treatment. Lactate dehydrogenase (LDH) used extent death induced luteolin. Flow-cytometry examine effect on cycle progression and mitochondrial membrane potential (ΛΨm) in cells. Phase-contrast microscopy detected morphological changes cells Our results demonstrated that dose-dependent as well time-dependent...
Telomere attrition is linked to cancer, diabetes, cardiovascular disease and aging. This because telomere losses trigger further genomic modifications, culminating with loss of cell function malignant transformation. However, factors regulating the transition from cells short telomeres, profoundly altered genomes, are little understood. Here, we use budding yeast engineered lack telomerase other forms maintenance, screen for such factors. We show that initially, different DNA damage...
Early detection of pancreatic cancer has been shown to improve patient survival rates. However, effective early tools detect do not currently exist. The Avantect Pancreatic Cancer Test, leveraging the 5-hydroxymethylation [5-hydroxymethylcytosine (5hmC)] signatures in cell-free DNA, was developed and analytically validated address this unmet need. We report a comprehensive analytical validation study encompassing precision, sample stability, limit detection, interfering substance studies,...
Parthenogenetic embryonic stem cells ( P g ES ) might advance cell replacement therapies and provide a valuable in vitro model system to study the genomic imprinting. However, differential potential of was limited. It could result from relative low heterology compared with fertilization (f ), which produce different expression most imprinted genes. Here, we described establishment by aggregating parthenogenetic embryos at 8‐cell stage (a cells), may increase heterozygy. We found that...
Abstract Background Over the years, long non-coding RNAs (lncRNAs) have been clarified in malignancies, this research was focused on role of lncRNA cartilage injury-related (lncRNA-CIR) osteosarcoma cells. Methods LncRNA-CIR expression tissues and cells, adjacent normal osteoblasts determined, then relations between lncRNA-CIR clinicopathological features, prognosis patients were analyzed. Moreover, MG63 143B cells treated with silenced or overexpressed lncRNA-CIR, proliferation, invasion,...
Monocrotaline (MCT), a pyrrolizidine alkaloid extracted from the shrub Crotalaria spectabilis, induces in lungs of many mammalian species severe hypertension and fibrosis. Previous work with MCT-induced lung disease rats has shown that some steps to progressive fibrosis can be interrupted or decreased by intervention retinoic acid (RA) angiotensin converting enzyme inhibitor, captopril. This report emphasizes pathology cytokines present MCT model 8 treatment groups, six per group: (1)...
We have previously demonstrated that cigarette smoke is associated with a significant reduction of retinoic acid in rat lungs and the formation tracheal precancerous lesions. However, underlying mechanism cancer risk induced by vitamin A deficiency unclear. The purpose this study was to determine whether smoke-induced depletion related changes lung risk-related molecular markers.We investigated roles receptors (RARs) as well other biomarkers for potential rats exposed smoke. Twenty-four male...
Replicative senescence is a permanent cell cycle arrest in response to extensive telomere shortening. To understand the mechanisms behind arrest, we screened for factors affecting replicative budding yeast lacking elongation pathways. Intriguingly, found that DNA polymerase epsilon (Pol ε) acts synergistically with Exo1 nuclease maintain senescence. In contrast, Pol ε-associated checkpoint and replication protein Mrc1 facilitates escape from this paradox, which DNA-synthesizing cooperate...
Shikonin is a naphthoquinone compound extracted from the Chinese herb purple gromwell. has broad antibacterial, anti-inflammatory, and antitumor activities. The tumor necrosis factor-α (TNF-α)-induced proliferation invasion of vascular smooth muscle cells (VSMCs) an important factor that contributes to atherosclerosis. effects shikonin on apoptosis VSMCs have been reported; however, function TNF-α-mediated growth during atherosclerosis remains unclear. In this study, we used Western blot,...
In the present study, third‑generation autologous‑inactivated bone morphogenic protein 2 (BMP2), BMP4, BMP6, BMP7, BMP9 and Wnt3a lentiviral vectors were constructed integrated into genome of MC3T3‑E1 murine mesenchymal stem cells (MMSCs) to produce osteoinductive factor gene‑modified MMSCs. The transfection efficiency each was then determined by detecting expression levels runt related transcription (Runx2) mRNA. cotransfection with combinations two lentiviruses performed,...
Research has indicated that excessive vitamin A can have deleterious impacts on bone. Retinoic acid (RA), the most active metabolite of A, been tested in clinical trials for treatment lung cancer and emphysema. These are not measuring Bone Mineral Content (BMC) or Density (BMD). In this study, we used an animal model to determine potential effects all-trans RA bone mass when as a means protect against treat cigarette smoke-induced injuries, also evaluate BMC early indicator osteoporosis...
Rif1 mediates telomere length, DNA replication, and damage responses in budding yeast. Previous work identified several posttranslational modifications of Rif1, however none these was shown to mediate the molecular or cellular damage, including damage. We searched for such using immunoblotting methods cdc13-1 tlc1Δ models found that is phosphorylated during serines 57 110 within a novel phospho-gate domain (PGD) are important this modification, cells. The phosphorylation appeared inhibit its...
Previously we found that cigarette smoke depleted vitamin A in the lungs and induced tracheal precancerous lesions.To understand molecular consequences underlying smoke-induced depletion its associated lung cancer risk, this study investigated cancer-related genes smoke-exposed rats with or without dietary retinoic acid, active metabolite of A. Twenty-four male weanling were fed either a control acid supplemented diet.Half each group was exposed to 40 commercial cigarettes/ d, 5 d/ wk.After...