A5047492762- Cancer, Hypoxia, and Metabolism
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Cancer Research and Treatments
- Mitochondrial Function and Pathology
- Gastric Cancer Management and Outcomes
- Molecular Biology Techniques and Applications
- Helicobacter pylori-related gastroenterology studies
- Cancer Cells and Metastasis
- Genetic factors in colorectal cancer
- Glioma Diagnosis and Treatment
- Cancer Treatment and Pharmacology
- ATP Synthase and ATPases Research
- Brain Metastases and Treatment
- Lung Cancer Treatments and Mutations
- Monoclonal and Polyclonal Antibodies Research
- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- Diet and metabolism studies
- HER2/EGFR in Cancer Research
- Cytokine Signaling Pathways and Interactions
- Digestive system and related health
- Dietary Effects on Health
- FOXO transcription factor regulation
- PARP inhibition in cancer therapy
UCLouvain
2019-2024
Candiolo Cancer Institute
2016-2024
Walloon Excellence in Lifesciences and Biotechnology
2022
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2018
University of Turin
2015-2018
University of Coimbra
2014
Abstract Gastric cancer is the world's third leading cause of mortality. In spite significant therapeutic improvements, clinical outcome for patients with advanced gastric poor; thus, identification and validation novel targets extremely important from a point view. We generated wide, multilevel platform models, comprising 100 patient-derived xenografts (PDX), primary cell lines, organoids. Samples were classified according to their histology, microsatellite stability, Epstein–Barr virus...
To successfully generate distant metastases, metastatic progenitor cells must simultaneously possess mesenchymal characteristics, resist to anoïkis, migrate and invade directionally, redox shear stresses in the systemic circulation, stem cell characteristics. These primarily originate from metabolically hostile areas of primary tumor, where oxygen nutrient deprivation, together with metabolic waste accumulation, exert a strong selection pressure promoting evasion. Here, we followed...
To survive and proliferate in solid tumors, cancer cells adapt evolve rapidly microenvironments where oxygen substrate bioavailability fluctuates over time space. This creates metabolic heterogeneity. Cancer can further cooperate metabolically, for example by swapping glycolytic end-product lactate blood-borne glucose. type of cooperation be targeted therapeutically, since transmembrane exchanges are facilitated lactate-proton symporters the monocarboxylate (MCT) family. Among new drugs,...
At diagnosis, about 35% of pancreatic cancers are at the locally invasive yet premetastatic stage. Surgical resection is not a treatment option, leaving patients with largely incurable disease that often evolves to polymetastatic stage despite chemotherapeutic interventions. In this preclinical study, we hypothesized cancer metastasis can be prevented by inhibiting mitochondrial redox signaling MitoQ, mitochondria-targeted antioxidant. Using four different cell lines, report that, clinically...
Abstract Background Growing evidence supports the use of low-carbohydrate/high-fat ketogenic diets as an adjunctive cancer therapy. However, it is unclear which genetic, metabolic, or immunological factors contribute to beneficial effect diets. Therefore, we investigated on progression and metabolism genetically metabolically heterogeneous melanoma xenografts, well development metastases in mice with a functional immune system. Methods Mice bearing BRAF mutant, NRAS wild-type xenografts...
In oncology, the occurrence of distant metastases often marks transition from curative to palliative care. Such outcome is highly predictable for breast cancer patients, even if tumors are detected early, and there no specific treatment prevent metastasis. Previous observations indicated that cell mitochondria bioenergetic sensors tumor microenvironment produce superoxide promote evasion. Here, we tested whether mitochondria-targeted antioxidant MitoQ capable metastasis in MDA-MB-231 model...
Patient-Derived Xenografts (PDXs), entailing implantation of cancer specimens in immunocompromised mice, are emerging as a valuable translational model that could help validate biologically relevant targets and assist the clinical development novel therapeutic strategies for gastric cancer. More than 30% PDXs generated from carcinoma samples developed human B-cell lymphomas instead These were monoclonal, Epstein Barr Virus (EBV) positive, originated tumorigenic cell cultures displayed...
Treatment of myocardial infarction with mesenchymal stem cells (MSCs) has proven beneficial effects in both animal and clinical studies. Engineered silica nanoparticles (SiO2-NPs) have been extensively used as contrast agents regenerative medicine, due to their resistance degradation ease functionalization. However, there are still controversies on effective biosafety cellular systems. In this perspective, the aims present study are: 1) deeply investigate impact amorphous 50 nm SiO2-NPs...
Distant metastases are detrimental for cancer patients, but the increasingly early detection of tumors offers a chance metastasis prevention. Importantly, cancers do not metastasize randomly: depending on type cancer, metastatic progenitor cells have predilection well-defined organs. This has been theorized by Stephen Paget, who proposed "seed-and-soil hypothesis", according to which colonization occurs only when needs given cell (the seed) match with resources provided organ soil). Here, we...
Abstract Background Gastric cancer (GC) is the third leading cause of cancer-related death worldwide, with a poor prognosis for patients advanced disease. Since oncogenic role KRAS mutants has been poorly investigated in GC, this study aims to biochemically and biologically characterize different KRAS-mutated models unravel differences among response therapy. Methods Taking advantage proprietary, molecularly annotated platform more than 200 GC PDXs (patient-derived xenografts), we identified...
Abstract In oncology, the occurrence of distant metastases often marks transition from curative to palliative care. Such outcome is highly predictable for triple-negative and HER2-positive breast cancer patients, even if tumors are detected early, there no treatment prevent metastasis. Here, considering that mitochondria bioenergetic sensors tumor microenvironment, we report in preclinical mouse models human relapse not a fatality: local recurrence metastatic dissemination can be prevented...
Abstract Hypoxic tumors are radioresistant stemming from the fact that oxygen promotes reactive species (ROS) propagation after water radiolysis and stabilizes irradiation-induced DNA damage. Therefore, an attractive strategy to radiosensitize solid is increase tumor oxygenation at time of irradiation, ideally above a partial pressure 10 mm-Hg which full radiosensitization can be reached. Historically, many attempts vascular O 2 delivery have had limited efficacy, but mathematical models...
Abstract Reactive oxygen species (ROS) are well known to play important roles in cancer. In particular, ROS of mitochondrial origin (mtROS) promote DNA mutations and cell death when produced at high levels, but rather stimulate autophagy metastatic spread subcytotoxic levels [1]. Multiple metabolic pathways oncogenic regulators linked have been studied cancer cells, including MAPK/ERK, PI3K/AKT, PTEN, PKD, BREF2, HIF-1, NF-κB p53 [2]. Abnormalities these can contribute tumor development.By...
Abstract Gastric cancer is the world third leading cause of mortality. In spite significant therapeutic advances, overall clinical outcome for patients with advanced gastric poor, 5-20% 5-year survival. The only targeted therapy approved so far are trastuzumab, and Ramucirumab which have given unsatisfactory results. Around 50% tumors bear genetic alterations affecting tyrosine kinase pathways (mainly EGFR, HER3, FGFR2 MET pathways, besides HER2) but their validation as tumor drivers...