A5056044582- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Carcinogens and Genotoxicity Assessment
- Tissue Engineering and Regenerative Medicine
- Bladder and Urothelial Cancer Treatments
- CRISPR and Genetic Engineering
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- 3D Printing in Biomedical Research
- Genomics and Chromatin Dynamics
- Advanced biosensing and bioanalysis techniques
- Mesenchymal stem cell research
- Nanoplatforms for cancer theranostics
- T-cell and B-cell Immunology
- Renal and related cancers
- Telomeres, Telomerase, and Senescence
- Graphene and Nanomaterials Applications
- Polyomavirus and related diseases
- Neuroblastoma Research and Treatments
- Genomic variations and chromosomal abnormalities
- Extracellular vesicles in disease
University of Turin
2008-2018
Phosphorylation of histone H2AX (γH2AX) is known to be the earliest indicator DNA double-strand breaks. Recently, it has been shown that mouse embryonic stem cells (mESCs) have very high basal levels γH2AX, even when they not exposed genotoxic agents. As specialized role γH2AX in pluripotent still debated, we investigated whether phosphorylation important maintaining self-renewal these cells. Here, report only mESCs but also mouse-induced (miPSCs), γH2AX. We show decrease upon ESC and iPSC...
Human mesenchymal stem cells (hMSCs) are adult multipotent located in various tissues, including the bone marrow. In contrast to terminally differentiated somatic cells, must persist and function throughout life ensure tissue homeostasis repair. For this reason, they be equipped with DNA damage responses able maintain genomic integrity while ensuring their lifelong persistence. Evaluation of hMSC response genotoxic insults is great interest considering both therapeutic potential...
Abstract Ataxia telangiectasia (A‐T) is a progressive neurodegenerative disease with onset in early childhood, caused by mutations the ATM (ataxia‐telangiectasia mutated) gene. Diagnosis relies on laboratory tests showing high levels of serum alphafetoprotein, cell sensitivity to ionizing radiation (IR) and absence or reduced protein. Many tests, however, are not sufficiently sensitive specific for A‐T, have long turnaround times, require large blood samples. This prompted us develop new...
Highly bright and photostable cyanine dye-doped silica nanoparticles, IRIS Dots, are developed, which can efficiently label human mesenchymal stem cells (hMSCs). The application procedure used to hMSCs is fast (2 h), the concentration of Dots for efficient labeling low (20 μg mL(-1) ), labeled be visualized by flow cytometry, confocal microscopy, transmission electron microscopy. Labeled unaffected in their viability proliferation, as well stemness surface marker expression differentiation...
Aim: To assess functional effects of silica nanoparticles (SiO2-NPs) on human mesenchymal stem cell (hMSC) cardiac integration potential. Methods: SiO2-NPs were synthesized and their internalization hMSCs analyzed with particular emphasis interaction the environment Results: SiO2-NP affected area maturation level hMSC focal adhesions, accounting for increased in vitro adhesion capacity augmented engraftment myocardial tissue upon injection infarcted isolated rat hearts. treatment also...
The biomaterial scaffold plays a key role in most tissue engineering strategies. Its surface properties, micropatterning, degradation, and mechanical features affect not only the generation of construct vitro, but also its vivo functionality. area myocardial still faces significant difficulties challenges design bioactive scaffolds, which allow composition variation to accommodate divergence evolving structure. Here we aimed at verifying if microstructured bioartificial alone can provoke an...
In patients affected by Ataxia-Telangiectasia (A-T), mutations in the ATM gene lead to loss-of-function alleles. Nonsense, splice-site variants, small insertions or deletions (frameshifts) and missense are most commonly found mutations. Large genomic (LGDs) rare (∼1%) but can same phenotype. compound heterozygotes, not detected screening strategies. We analysed 12 unrelated Italian A-T identified all 24 mutated Twelve were novel. Standardized SNP STR haplotyping followed DHPLC of DNA,...
Treatment of myocardial infarction with mesenchymal stem cells (MSCs) has proven beneficial effects in both animal and clinical studies. Engineered silica nanoparticles (SiO2-NPs) have been extensively used as contrast agents regenerative medicine, due to their resistance degradation ease functionalization. However, there are still controversies on effective biosafety cellular systems. In this perspective, the aims present study are: 1) deeply investigate impact amorphous 50 nm SiO2-NPs...
Recent studies have shown that mouse embryonic stem cells (mESCs) rely on a distinctive genome caretaking network. In this review, we will discuss how mESCs functionally respond to DNA damage and describe several modifications in mESC response, which accommodate dynamic cycling preservation of genetic information. Subsequently, the transition from adult stem/progenitor can be involved decline tissue integrity function elderly.
Bladder cancer (BC) has a typical aetiology characterized by multistep carcinogenesis due to environmental exposures, genetic susceptibility, and their interaction. Several lines of evidence suggest that DNA repair plays role in the development progression BC. In particular, study individual susceptibility double strand breaks (DSBs) may provide valuable information on BC risk, help identify those patients at high-risk either recurrence or disease, possibly personalizing both surveillance...
Abstract Background Current chemotherapy of human cancers focuses on the DNA damage pathway to induce a p53-mediated cellular response leading either G1 arrest or apoptosis. However, genotoxic treatments may mutations and translocations that result in secondary malignancies recurrent disease. In addition, about 50% are associated with p53 gene. Nongenotoxic activation apoptosis by targeting specific molecular pathways thus provides an attractive therapeutic approach. Methods Normal leukemic...