A5101857048- Research on Leishmaniasis Studies
- Toxin Mechanisms and Immunotoxins
- Trypanosoma species research and implications
- vaccines and immunoinformatics approaches
- Transgenic Plants and Applications
- Synthesis and Biological Evaluation
- Intensive Care Unit Cognitive Disorders
- Vaccine Coverage and Hesitancy
- Meningioma and schwannoma management
- Neonatal Respiratory Health Research
- Insect Pest Control Strategies
- Monoclonal and Polyclonal Antibodies Research
- Vestibular and auditory disorders
- Infection Control in Healthcare
- Respiratory Support and Mechanisms
- Ophthalmology and Eye Disorders
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- Emergency and Acute Care Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Nosocomial Infections in ICU
Rajendra Memorial Research Institute of Medical Sciences
2005-2022
Indian Council of Medical Research
2005
University of Southern California
1997
University of Illinois Chicago
1990
Harvard University
1972
Massachusetts General Hospital
1972
Kumar, A.; Falke, K. J.; Geffin, B.; Aldredge, C. F.; Laver, M. LOWENSTEIN, E.; PONTOPPIDAN, H. Author Information
Visceral leishmaniasis (VL) is one of the most neglected tropical diseases for which no vaccine exists. In spite extensive efforts, successful available against this dreadful infectious disease. To support development, an immunoinformatics approach was applied to screen potential MHC class-II-restricted epitopes that can activate immune cells. Initially, 37 derived from six stage-dependent, overexpressed antigens were predicted, presented by at least 26 diverse class-II allele. Based on a...
We evaluated 85 human immunodeficiency virus (HIV)—negative patients with tuberculosis for clinical features and CD4 cell counts. Thirty-seven had low counts (mean ± SD, 341 116 cells/µL), 48 normal 830 254 cells/µL). were most strongly correlated total lymphocyte (r = 0.84). If count was excluded, depressed significantly associated serum albumin levels, extensive pulmonary disease, body-mass index, hematocrit. Of these four variables, multivariate linear discriminant analysis revealed that...
Human visceral leishmaniasis (VL) or kala-azar remains a major cause of mortality, particularly in the developing world. The disease is common internal regions north–eastern India, which have tropical sub-tropical climate. In recent study on VL this region, relationship between incidence and certain physio–environmental factors was explored, using combination geographical information system (GIS), satellite imagery data collected 'on ground'. Some eco–environmental parameters were then used...
Abstract To explore new protective measure against visceral leishmaniasis, reverse vaccinology approach was employed to identify key immunogenic regions which can mediate long-term immunity. In-depth computational analysis revealed nine promiscuous epitopes possibly be presented by 46 human leukocyte antigen, thereby broadening the worldwide population up 94.16%. This is of reasonable significance that most shared 100% sequence homology with other Leishmania species and could evoke a common...
Although, the precise host defence mechanism(s) is not completely understood, T cell-mediated immune responses believed to play a pivotal role in controlling parasite infection. Here we target stage dependent over expressed gene. Here, consensus based computational approach was adopted for screening of potential major histocompatibility complex class I restricted epitopes. Based on analysis and previously published report, set 19 antigenic proteins derived from Leishmania donovani were...
Although the precise host-defence mechanisms are not completely understood, T-cell-mediated immune responses believed to play a pivotal role in controlling parasite infection. In this study, potential HLA*A2 restricted peptides were predicted and ability of bind HLA-A*02 was confirmed by MHC stabilization assay. Two tested stabilized HLA-A*02: (a) LLATTVSGL (P1) (b) LMTNGPLEV (P3). The generate protective response evaluated patients with treated visceral leishmaniasis as well healthy control...
Available reports suggest that, Leishmania donovani antigen KMP-11 may be significant in the modulation of immune responses visceral leishmaniasis (VL). This study evaluated vaccine prospect presentation through murine dendritic cells against VL infected BALB/c mice. We report here that immunization with delivered bone marrow derived can lead to killing L. Furthermore, strategy use as DCs stimulate production IFN-g, IL-12, IL-2R and TNF-α concomitant down-regulation IL-10 IL-4....
A simple and highly reproducible dot-immunoblot assay was developed to detect leishmanial antigen in Phlebotomus argentipes that were naturally infected with Leishmania donovani. The test sensitive as little 10 ng of antigenic protein (equivalent the gut content one laboratory-infected sandfly) also appeared be specific, it gave a positive result some P. (the primary vector L. donovani India) but not papatasi or other pathogens.When used investigate large number sandflies collected from two...
Visceral leishmaniasis is one of the most neglected tropical diseases for which no vaccine exists.In spite extensive efforts, successful available against this dreadful infectious disease.To support development, immunoinformatics approach was applied to search potential MHC-classII restricted epitopes that can activate immune cells.Initially, a total 37 derived from six, stage dependent over expressed antigens were predicted, presented by at least 26 diverse MHC class II alleles including:...
Vaccination is considered the most appropriate way to control visceral leishmaniasis (VL). With this background, r-LdODC protein as well its derived HLA-DRB1-restricted synthetic peptides (P1: RLMPSAHAI, P2: LLDQYQIHL, P3: GLYHSFNCI, P4: AVLEVLSAL, and P5: RLPASPAAL) were validated in BALB/c mice against leishmaniasis. The study was initiated by immunization of cocktail with adjuvants (r-CD2 MPL-A) different groups, separately. Splenocytes isolated from challenged differentially immunized...