The intestine requires a high amount of energy to maintain its health and function; thus, deficits in intestinal mucosa may lead damage. Asparagine (Asn) is precursor for many other amino acids such as aspartate, glutamine glutamate, which can be used supply enterocytes. In the present study, we hypothesise that dietary supplementation Asn could alleviate bacterial lipopolysaccharide (LPS)-induced injury via improvement status. A total twenty-four weaned piglets were assigned one four...

Ovarian cancer (OC) is a highly malignant with great metastatic potential. Here we aimed to investigate the role of Piezo1, gene related mechanical environment tumor, in promoting metastasis OC. We performed Piezo1 knockdown A-1847 cells using small hairpin RNAs, and were inoculated subcutaneously nude mice. decreased tumor growth rate OC xenografts mice reduced cell migration vitro. Metastasis lung was also attenuated after as revealed by HE staining tissues, which concomitant...

Vitamin D deficiency (VDD) increases the risk for type 2 diabetes mellitus (T2DM), which might be related to insulin resistance (IR). We aimed explore association between triglyceride-glucose (TyG) index, a reliable indicator of IR, and VDD in patients with T2DM.There were 1034 participants T2DM enrolled Second Xiangya Hospital Central South University. The TyG index was calculated as ln (fasting triglyceride (TG, mg/dL) × fasting blood glucose (mg/dL)/2). defined 25-hydroxyvitamin [25(OH)D]...

Respiratory syncytial virus (RSV) causes the most common type of severe lower respiratory tract infection worldwide, and fusion (F) protein is a target for neutralizing antibodies vaccine development. This study aimed to investigate immunogenicity efficacy an mRNA-based RSV with F sequence. We designed mRNA construct encoding modified protein, which was further developed into LNP-encapsulated (LVRNA007). LVRNA007 administered mice cotton rats, followed by analysis viral challenge studies....

Primary glaucoma patients faced many difficulties that affected their treatment during the COVID-19 pandemic. Glaucoma often starts with prescription eye-drops. However, delays in ophthalmic therapy occur due to poor awareness of dangers glaucoma, which subsequently leads irreversible visual field defects and eventual blindness. This study aimed explore social, personal medical factors contributed barriers primary pandemic, overarching goal providing insights develop measures can identify...

Placental CYP27B1 may contribute to circulating maternal calcitriol concentrations across gestation, but determinants of and CYP24A1 expression in term human placental tissue are not well established.We hypothesized that higher protein would be associated with increased during gestation impacted by substrate availability.This was a prospective, longitudinal study.The study completed an urban, prenatal clinic located Rochester, New York.The undertaken cohort 70 pregnant adolescents (≤18 y...

Age‑associated diseases, including vascular are on the rise with increase in aging population. However, mechanisms of and age‑associated dysfunction remain to be fully elucidated. Replicative senescence smooth muscle cells (VSMCs) contributes as well diseases. Rapamycin may delay aging‑associated diseases via inhibition mammalian target rapamycin (mTOR), but its role VSMC has remained elusive. The present study investigated involvement mTOR signaling replicative VSMCs. was induced human...

Abstract Long noncoding RNAs (lncRNAs) have been investigated as novel regulatory molecules involved in diverse biological processes. Our previous study demonstrated that lncRNA‐ES3 is associated with the high glucose–induced calcification/senescence of human aortic vascular smooth muscle cells (HA‐VSMCs). However, mechanism calcification/aging remained largely unknown. Here, we report expression basic helix‐loop‐helix family member e40 (Bhlhe40) was decreased significantly HA‐VSMCs treated...

Hyperhomocysteinemia (HHcy) has been linked to several clinical manifestations including chronic kidney disease.However, it is not known whether HHcy a role in the development of acute injury (AKI).In present study, we reported that mice developed more severe renal after cisplatin injection and ischemia-reperfusion shown as tubular damage higher serum creatinine.In response cisplatin, showed prevalent cell apoptosis decreased proliferation.Mechanistically, heightened ER stress reduced Akt...

Abstract Indoleamine 2,3-dioxygenase 1 (IDO1) is an enzyme for tryptophan metabolism, involved in immune cell differentiation/maturation and cancer biology. IDO1 also expressed cardiomyocytes, but its roles the cardiovascular system are not fully understood. Here, we reported functions of during cardiac hypertrophy. Quantitative real-time PCR Western blot experiments demonstrated upregulation mRNA protein levels human hypertrophic mouse hearts, as well angiotensin II (Ang II)-induced rat...

Summary Objectives To assess the relationship between body composition and fracture risk as determined by FRAX ® model in postmenopausal women central south China. Methods A cross‐sectional study was conducted 779 women. Bone mineral density ( BMD ) of left femur total were measured dual X ‐ray absorptiometry. All clinical information available for incorporation into to 10‐year probability. Results In model, when values included, probability major osteoporotic fractures hip 3·4% 1·0%,...

Axillary bromhidrosis is a condition presenting as malodor caused by an interaction between the discharge of apocrine glands and bacteria. Topical agents, liposuction, elective surgery are currently main therapeutic modalities. However, efficiency these treatments incidences side effects various frequent, depend on patient characteristics, surgical technique, other unknown factors.We report retrospective study outcomes in 396 patients treated mini-incision with subdermal vascular...

Abstract Objective Cardiovascular diseases and vascular aging are common in patients with diabetes. High glucose is a major cause of cardiovascular diseases. Premature senescence smooth muscle cells (VSMCs) one the main contributors to aging. Adiponectin has been demonstrated have an anti‐aging effect. The present study explored mechanisms by which adiponectin protects VSMCs against high‐glucose‐induced senescence. Methods Senescence‐associated β‐galactosidase (SA‐β‐gal) staining was used...

Our previous studies have shown that the basic helix-loop-helix family member e40 (Bhlhe40) plays a critical role in regulating calcification and senescence of vascular smooth muscle cells induced by high glucose. In this study, we determined association between serum Bhlhe40 levels subclinical atherosclerosis patients with type 2 diabetes mellitus (T2DM).247 T2DM were included cross-sectional study June 2021 July 2022. The presence was evaluated carotid ultrasonography. Serum concentrations...

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Osteoarthritis (OA) is a prevalent bone and joint disease characterized by degeneration. The dysregulation between chondrocyte synthesis breakdown key factor in OA development. Targeting the degenerative changes cartilage tissue degradation could be potential treatment approach for OA. Previous research has established strong link autophagy regulation of functions. Activating shown promise mitigating Currently, osteoarthritis primarily focuses on symptom management, as there no definitive...

Sepsis-induced cardiac dysfunction remains a major cause of morbidity and mortality in patients suffered from severe trauma. Mesenchymal stem cells (MSCs) -based treatment has been verified as promising approach to mitigate the sepsis-induced dysfunction, but mechanism is still ambiguous. Thus, our study was designed explore potential role MSCs dysfunction. In vivo bioluminescence imaging revealed 80% acute donor cell death bone marrow-derived (BM-MSCs) within 3 days after transplantation....

Abstract Background Fabry disease is a rare hereditary involving multiple organs, and there are few reports on how the initial manifestations renal involvement of these patients with classical late-onset phenotype evolve sexes ages. To improve clinicians’ understanding avoid misdiagnoses by discussing manifestations, first medical specialties visited development in patients. Methods This study collected relevant data from 311 Chinese (200 males, 111 females) descriptive statistical analysis...

Capsular characteristics of pleomorphic adenoma (PA) has various forms. Patients without complete capsule a higher risk recurrence than patients with capsule. We aimed to develop and validate CT-based intratumoral peritumoral radiomics models make differential diagnosis between parotid PA capsule.Data 260 (166 from institution 1 (training set) 94 (test 2) were retrospectively analyzed. Three Volume interest (VOIs) defined in the CT images each patient: tumor volume (VOItumor), VOIperitumor,...

Diabetic kidney disease (DKD) is one of the major microvascular complications diabetes. DKD associated with oxidative stress and inflammation. Versican (VCAN), a chondroitin sulphate proteoglycan, has been proven to participate in This study aimed explore overall sex-based relationship between serum VCAN levels albuminuria patients type 2 diabetes mellitus (T2DM). 428 T2DM 84 healthy individuals were enrolled. Patients separated into normal albuminuria, microalbuminuria, macroalbuminuria...