A5101788969- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- Herpesvirus Infections and Treatments
- Immunotherapy and Immune Responses
- Poxvirus research and outbreaks
- SARS-CoV-2 detection and testing
- Animal Virus Infections Studies
- Pleistocene-Era Hominins and Archaeology
- Advanced biosensing and bioanalysis techniques
- Geology and Paleoclimatology Research
- Archaeology and Rock Art Studies
- Protease and Inhibitor Mechanisms
- COVID-19 epidemiological studies
- Climate Change and Health Impacts
- Microbial infections and disease research
- Polymer Surface Interaction Studies
- Inflammasome and immune disorders
- MXene and MAX Phase Materials
- Bacteriophages and microbial interactions
- Biochemical and Structural Characterization
- Vector-Borne Animal Diseases
- TGF-β signaling in diseases
- vaccines and immunoinformatics approaches
- Dental Research and COVID-19
- Viral Infections and Outbreaks Research
University of Electronic Science and Technology of China
2024
Nanchang University
2023-2024
First Affiliated Hospital of Jiangxi Medical College
2024
Institute of Microbiology
2020-2023
Chongqing Cancer Hospital
2023
Chongqing University
2023
Shenzhen Technology University
2023
Shangdong Agriculture and Engineering University
2023
University of Chinese Academy of Sciences
2022
Beijing Normal University
2021
Monkeypox has been declared a public health emergency by the World Health Organization. There is an urgent need for efficient and safe vaccines against monkeypox virus (MPXV) in response to rapidly spreading epidemic. In age of COVID-19, mRNA have highly successful emerged as platforms enabling rapid development large-scale preparation. Here, we develop two MPXV quadrivalent vaccines, named mRNA-A-LNP mRNA-B-LNP, based on intracellular mature specific proteins (A29L M1R) extracellular...
Coronavirus disease 2019 (COVID-19) was first identified in late Wuhan, Hubei Province, China and spread globally months, sparking worldwide concern. However, it is unclear whether super-spreading events occurred during the early outbreak phase, as has been observed for other emerging viruses. Here, we analyse 208 publicly available SARS-CoV-2 genome sequences collected phase. We combine phylogenetic analysis with Bayesian inference under an epidemiological model to trace person-to-person...
mRNA-based therapy has emerged as the most promising nucleic acid in fight against COVID-19. However, a safe and efficacious systemic delivery remains challenge for mRNA therapy. Lipid nanoparticles (LNPs) are currently widely used vehicles. Here, series of ionizable LNPs is rationally designed. YK009-LNP an optimal platform to carry mRNA. exhibits higher efficiency, more favorable biodistribution pattern, better safety than approved MC3-LNP. In addition, encoding severe acute respiratory...
With the large number of atypical cases in mpox outbreak, which was classified as a global health emergency by World Health Organization (WHO) on 23 July 2022, rapid diagnosis and diseases with similar symptoms to such chickenpox respiratory infectious early stages viral infection is key controlling spread outbreak. In this study, antibodies against monkeypox virus A29L protein were efficiently rapidly identified combining mRNA immunization high-throughput sequencing individual B cells. We...
Antimicrobial peptides (AMPs) have gained increasing attention, as they can overcome recurring microbial invasions. However, their poor antimicrobial activity and potential cytotoxicity remain impediments to clinical applications novel therapeutic agents. To enhance the cell selectivity of AMPs, a series amphiphilic based on leucocin A were designed by substituting noncharged hydrophilic residues with arginine leucine. Of engineered peptides, peptide 7 (WRL3) (WLRAFRRLVRRLARGLRR-NH2)...
Abstract Messenger RNA (mRNA) transfection is the prerequisite for application of mRNA‐based therapeutics. In hard‐to‐transfect cells, such as macrophages, effective mRNA remains a long‐standing challenge. Herein, smart DNA‐based nanosystem reported containing ribosome biogenesis‐promoting siRNA, realizing efficient in macrophages. Four monomers are copolymerized to form nanoframework (NF), including N ‐isopropylacrylamide (NIPAM) skeleton and acrydite‐DNA initiator trigger cascade assembly...
Spleen and lymphoid organs are important targets for messenger RNA (mRNA) delivery in various applications. Current nanoparticle methods rely on drainage to lymph nodes from intramuscular or subcutaneous injections. In difficult-to-transfect antigen-presenting cells (APCs), such as dendritic (DCs), effective mRNA transfection remains a significant challenge. this study, lymphatic targeting carrier using DC membranes is developed, that efficiently migrated organs, the spleen nodes. The...
Background: Since the outbreak of mpox in 2022, disease has spread rapidly worldwide and garnered significant public attention. Vaccination is regarded as an effective measure to prevent mpox. The success COVID-19 mRNA vaccine demonstrates that mRNA-based vaccines represent a rapid multifunctional platform with considerable potential, are expected be strategy address spread. Methods: In this study, we screened multi-antigen-tandem bivalent candidate: lipid nanoparticle-encapsulated mRNA-1017...
Abstract The extreme instability of mRNA makes the practical application mRNA‐based vaccines heavily rely on efficient delivery system and cold chain transportation. Herein, a DNA‐based nanomachine, which achieves programmed capture, long‐term storage without cryopreservation, in cells, is developed. polythymidine acid (Poly‐T) functionalized poly( N ‐isopropylacrylamide) (DNA‐PNIPAM) synthesized assembled as central compartment nanomachine. DNA‐PNIPAM nano‐assembly exhibits reversible...
Abstract There is an urgent need for efficient and safe vaccines against the monkeypox virus (MPXV) in response to rapidly spreading epidemic. In age of COVID-19, mRNA have been highly successful emerged as platforms enabling rapid development large-scale preparation. Here, we developed two MPXV quadrivalent vaccines, named mRNA-A-LNP mRNA-B-LNP, based on IMVs (A29L M1R) EEVs (A35R B6R). By administering mRNA-B-LNP intramuscularly twice, mice induced MPXV-specific IgG antibodies potent...
A series of stringent non-pharmacological and pharmacological interventions were implemented to contain the pandemic but continues. Moreover, vaccination breakthrough infection reinfection in convalescent coronavirus disease 2019 (COVID-19) cases have been reported. Further, severe acute respiratory syndrome 2 (SARS-CoV-2) variants emerged with mutations spike (S) gene, target most current vaccines. Importantly, exhibit a trend immune escape from vaccination. Herein scientific question that...
Two doping modes of B, direct on the Ru surface and into CNT, both modulate electron density for high intrinsic activity, while latter further improves charge transfer efficiency to accelerate reaction kinetics.
To investigate the potential of DNA nanoparticles (DNPs) as carriers and adjuvants for mRNA vaccines. Customized oligonucleotides were assembled into tetrahedra (DNA-TH), which subsequently complexed with streptavidin encoding green fluorescent protein (GFP). Various assays conducted to evaluat stability DNPs, their cellular uptake, immune activation potential, GFP transcription efficiency. P53-mutant HSC-3 cells used establish a subcutaneous xenograft tumor model explore effects DNPs in...
Vaccines are used to protect human beings from various diseases. mRNA vaccines simplify the development process and reduce production cost of conventional vaccines, making it possible respond rapidly acute severe diseases, such as coronavirus disease 2019. In this study, a universal integrated platform for streamlined on-demand preparation products directly DNA templates was established. Target were amplified in vitro by polymerase chain reaction module transcribed into sequences, which...
<sec> <title>BACKGROUND</title> The COVID-19 pandemic has caused severe challenges while providing a unique opportunity to drive the rapid evolvement of higher education, including dentistry. Dental education needs transition toward an increasingly flexible and adaptable approach, such as blended teaching, better prepare for current beyond. </sec> <title>OBJECTIVE</title> This study provides perspectives senior undergraduate dental students teaching during post identifies modifiable factors...
Cherry Valley breeder ducks in Shandong province northern China have experienced swollen eyes, lachrymation, pox scabs on glabrous or skin, depression and dysentery since 2021. The spread of this infectious disease has seriously affected the major duck farms China. virus causing clinical signs was isolated by chicken embryo inoculation. We successfully a strain from diseased replication. also conducted experiments for using strains to infect ducklings. By comparison with 22 viruses already...