A5103134357- Poxvirus research and outbreaks
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Galectins and Cancer Biology
- RNA modifications and cancer
- RNA Research and Splicing
- Herpesvirus Infections and Treatments
- Protein Structure and Dynamics
- Virus-based gene therapy research
- Biochemical and Molecular Research
- Toxin Mechanisms and Immunotoxins
- Neurogenetic and Muscular Disorders Research
- Endoplasmic Reticulum Stress and Disease
- Enzyme Production and Characterization
- Plant Virus Research Studies
- Glycosylation and Glycoproteins Research
- Heat shock proteins research
- Amino Acid Enzymes and Metabolism
- Signaling Pathways in Disease
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Macrophage Migration Inhibitory Factor
- Pharmacological Effects of Natural Compounds
- ATP Synthase and ATPases Research
University of Würzburg
2016-2025
European Molecular Biology Laboratory
2007-2010
Philipps University of Marburg
2000-2006
Max Planck Institute of Biophysics
2002
The crystal structure of 3α-hydroxysteroid dehydrogenase/carbonyl reductase from <i>Comamonas testosteroni</i> (3α-HSDH) as well the its binary complex with NAD<sup>+</sup> have been solved at 1.68-Å and 1.95-Å resolution, respectively. enzyme is a member short chain dehydrogenase/reductase (SDR) family. Accordingly, active center conformation bound nucleotide cofactor closely resemble those other SDRs. reveals one homodimer per asymmetric unit representing physiologically unity....
Abstract Background/objectives Hearing loss (HL) is one of the most common congenital disorders, affecting 1-2 in 1,000 newborns. Modern genetic diagnostics using large gene panels and/or whole exome analysis (WES) can identify disease-causing mutations 25-50 % patients, with higher solve rates individuals earlier onset. Results Here, we used whole-genome sequencing (WGS) to reanalyze 14 index patients/families who remained without diagnosis by WES. We were able cause HL 6 families (...
Sucrose phosphorylases have been applied in the enzymatic production of glycosylated compounds for decades. However, several desirable acceptors, such as flavonoids or stilbenoids, that exhibit diverse antimicrobial, anticarcinogenic antioxidant properties, remain poor substrates. The Q345F exchange sucrose phosphorylase from Bifidobacterium adolescentis allows efficient glucosylation resveratrol, (+)-catechin and (-)-epicatechin yields up to 97 % whereas wild-type enzyme favours hydrolysis....
Accumulating evidences have assigned a central role to parasite-derived proteins in immunomodulation. Here, we report on the proteomic identification and characterization of immunomodulatory excretory-secretory (ES) products from metacestode larva (tetrathyridium) tapeworm Mesocestoides corti (syn. M. vogae). We demonstrate that ES but not larval homogenates inhibit stimuli-driven release pro-inflammatory, Th1-inducing cytokine IL-12p70 by murine bone marrow-derived dendritic cells (BMDCs)....
Poxviruses are large DNA viruses with a linear double-stranded genome circularized at the extremities. The helicase-primase D5, composed of six identical 90 kDa subunits, is required for replication. D5 consists primase fragment flexibly attached to hexameric C-terminal polypeptide (res. 323–785) confirmed nucleotide hydrolase and DNA-binding activity but an elusive helicase activity. We determined its structure by single-particle cryo-electron microscopy. It displays AAA+ core flanked N-...
Here we present a point mutation-triggered domain shift which switches the acceptor preference of sucrose phosphorylase from phosphate to variety large polyphenolic compounds including resveratrol and quercetin, enabling their efficient glucosylation. The variant possesses high affinity for aromatic substrates due newly introduced π-π- hydrophobic interactions in altered active site. brings about substantially enlarged multifunctional site polyphenol glucosylation rare disaccharide...
Malfunction of pre-mRNA processing factors are linked to several human diseases including cancer and neurodegeneration. Here we report the identification a de novo heterozygous missense mutation in SNRPE gene (c.65T>C (p.Phe22Ser)) patient with non-syndromal primary (congenital) microcephaly intellectual disability. encodes SmE, basal component U snRNPs. We show that microcephaly-linked SmE variant is unable interact SMN complex as consequence fails assemble into This results widespread mRNA...
The year 2022 was marked by the mpox outbreak caused human monkeypox virus (MPXV), which is approximately 98% identical to vaccinia (VACV) at sequence level with regard proteins involved in DNA replication. We present production baculovirus-insect cell system of VACV polymerase holoenzyme, consists E9 combination its co-factor, A20-D4 heterodimer. This led 3.8 Å cryo-electron microscopy (cryo-EM) structure DNA-free form holoenzyme. model holoenzyme constructed from high-resolution structures...
A recent publication by Seng et al. in this journal reports the crystallographic structure of refolded, full-length SMN protein and two disease-relevant derivatives thereof. Here, we would like to suggest that at least structures reported study are incorrect. We present evidence one associated datasets is derived from a crystal bacterial Sm-like Hfq second dataset Gab protein. Both proteins frequent contaminants bacterially overexpressed which might have been co-purified during metal...
Formyltransferase catalyzes the reversible formation of formylmethanofuran from N(5)-formyltetrahydromethanopterin and methanofuran, a reaction involved in C1 metabolism methanogenic sulfate-reducing archaea. The crystal structure homotetrameric enzyme Methanopyrus kandleri (growth temperature optimum 98 degrees C) has recently been solved at 1.65 A resolution. We report here structures formyltransferase Methanosarcina barkeri 37 Archaeoglobus fulgidus 83 1.9 2.0 resolution, respectively....