A5004939340- Viral Infections and Vectors
- Insect Resistance and Genetics
- Vector-Borne Animal Diseases
- RNA Interference and Gene Delivery
- interferon and immune responses
- Animal Disease Management and Epidemiology
- Cell death mechanisms and regulation
- Antimicrobial Peptides and Activities
- Influenza Virus Research Studies
- TGF-β signaling in diseases
- Aquaculture disease management and microbiota
- Invertebrate Immune Response Mechanisms
- Immune Response and Inflammation
- Viral Infectious Diseases and Gene Expression in Insects
- Antibiotic Resistance in Bacteria
- Viral Infections and Immunology Research
- Monoclonal and Polyclonal Antibodies Research
- Bacterial biofilms and quorum sensing
- Insect Pest Control Strategies
- GDF15 and Related Biomarkers
- Connective Tissue Growth Factor Research
- Biochemical and Structural Characterization
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Virology and Viral Diseases
Southwest University
2013-2024
State Key Laboratory of Silkworm Genomic Biology
2013-2016
Gansu Agricultural University
2007
Apoptosis is a known regulator of morphogenetic events. In mammals, the critical role oxidative stress-induced apoptosis has been well-studied; however, in insects stress not clear. previous study, we showed that apoptosis-related genes are present silkworm Bombyx mori, an important lepidopteran insect model. this evaluated effect H2O2-induced on apoptosis, reactive oxygen species (ROS) levels, mitochondrial response, cytochrome c release and gene expression BmN-SWU1 cell line from B. mori...
Background: Klebsiella pneumoniae is an important human pathogen that causes severe diseases including urinary tract infection, pneumonia, and bacteremia. However, a rapid sensitive detection method remains to be developed. Objectives: This study aimed develop rapid, real-time, visual for K. pneumoniae. primary screening improve the diagnosis of infection save much precious time clinical practice. Methods: was used as antigen produce monoclonal antibodies (mAb). The mAb 1E6 recognizing outer...
Abstract Caspases are a family of cysteinyl aspartate proteinases known for their essential roles in the regulation apoptosis. To date, ≈20 caspases have been identified, although few cloned and characterized Bombyx mori (L.). Two caspase-9 variants from B. embryo cell line (BmE), designated BmDroncL BmDroncS, were cloned. has three classic domains: caspase recruitment domain large small subunits, is homologue Drosophila Dronc, human caspase-9, Aedes fish whereas BmDroncS lacks subunit....
Abstract A histidine (His)-tag is composed of six His residues and typically exerts little influence on the structure solubility expressed recombinant fusion proteins. Purification methods for proteins containing His-tags are relatively well-established, thus widely used in protein recombination technology. We established a one-step enzyme-linked immunosorbent assay (ELISA) His-tagged analyzed variable heavy light chains anti-His-tag monoclonal antibody 4C9 BLAST analyses to determine zones...
Investigate the function of BmICE-2 gene in silkworm cells by constructing a interference vector and analysing its effect. Online RNAi design software was used to predict target sequence Bmice-2. An optimized selected inserted into backbone microRNA. The vector, microRNA-siBmICE-2, control microRNA-siBmICE-2-control were designed. synthesized segment digested Asc I Sac II, PIZ/V5-dsRed vector. PIZ/V5-BmICE-2-miRNA-dsRed PIZ/V5-BmICE-2-Control-miRNA-dsRed designed validated through digestion...
本研究旨在阐明荣昌猪BMP15基因在荣昌猪性成熟前不同发育期的表达特性及其与SMADs信号相关基因的表达关系。采集1月龄、3月龄及5月龄荣昌猪卵巢组织，利用qRT-PCR及石蜡切片免疫荧光染色法分析荣昌猪不同月龄卵巢组织内BMP15基因表达及细胞定位特征；利用5月龄卵巢活组织添加重组人BMP15蛋白及TGF-β受体抑制剂（LY215799和LY2109761），应用qRT-PCR及Western blot方法分析BMP15及SMADs信号通路相关基因SMAD2、SMAD3、SMAD4、SMAD7、TGF-β1、TGF-β2、TGF-β3、TGF-β RⅠ和TGF-β RⅡ表达特征及BMP15/SMADs信号通路。结果表明：从1月龄至5月龄，随着荣昌猪生长发育，卵巢组织内BMP15基因mRNA表达量呈上调表达（P < 0.05）；石蜡切片免疫荧光试验表明，5月龄卵巢组织卵母细胞周围颗粒细胞内存在BMP15蛋白荧光信号；从3月龄至5月龄的卵巢组织内BMP15、SMAD4、TGF-β1及TGF-β RⅡ基因在mRNA水平呈上调表达（P...
An indirect enzyme-linked immunosorbent assay (iELISA) was established to detect the serological prevalence of bluetongue virus (BTV) infection in ruminant populations.A recombinant NS4 (rNS4) protein used as encapsulated antigen.Optimization iELISA included antigen, serum dilution, blocking solution, and working concentration a horseradish peroxidase (HRP)-labeled secondary antibody (Ab) by square-matrix titration test.The results showed that optimal reaction conditions for were: volume...