A5101966406- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Hearing, Cochlea, Tinnitus, Genetics
- Protein Degradation and Inhibitors
- Adenosine and Purinergic Signaling
- Ion channel regulation and function
- Single-cell and spatial transcriptomics
- Advanced Biosensing Techniques and Applications
- Synthesis and Biological Evaluation
- Antimicrobial Peptides and Activities
- Acoustic Wave Phenomena Research
- Microfluidic and Bio-sensing Technologies
- Neuroscience and Neuropharmacology Research
- Histone Deacetylase Inhibitors Research
- Electrochemical Analysis and Applications
- Multiple Myeloma Research and Treatments
- Natural product bioactivities and synthesis
- Bacterial biofilms and quorum sensing
- Ion Channels and Receptors
- Receptor Mechanisms and Signaling
- Genomics, phytochemicals, and oxidative stress
- Endoplasmic Reticulum Stress and Disease
- Redox biology and oxidative stress
- Metabolomics and Mass Spectrometry Studies
- Connexins and lens biology
Sichuan University
2024-2025
Guizhou University
2023-2024
Tsinghua University
2024
Fourth Affiliated Hospital of Guangxi Medical University
2024
Chinese Academy of Sciences
2023-2024
Beijing Institute of Nanoenergy and Nanosystems
2024
Hong Kong University of Science and Technology
2017-2023
University of Hong Kong
2017-2023
Guangzhou Medical University
2012-2023
Shenzhen Institutes of Advanced Technology
2023
Abstract Targeted protein degradation via “hijacking” of the ubiquitin-proteasome system using proteolysis targeting chimeras (PROTACs) has evolved into a novel therapeutic modality. The design PROTACs is challenging; multiple steps involved in PROTAC-induced make it difficult to establish coherent structure-activity relationships. Herein, we characterize PROTAC-mediated ternary complex formation and by employing von Hippel–Lindau (VHL) recruiting for two different target proteins, SMARCA2...
Abstract Precious metals are core assets for the development of modern technologies in various fields. Their scarcity poses question their cost, life cycle and reuse. Recently, an emerging catalysis employing contact-electrification (CE) at water-solid interfaces to drive redox reaction, called contact-electro-catalysis (CEC), has been used develop metal free mechano-catalytic methods efficiently degrade refractory organic compounds, produce hydrogen peroxide, or leach from spent Li-Ion...
// Ningning Liu 1,2,* , Xiaofen Li 1,* Hongbiao Huang Chong Zhao Siyan Liao Changshan Yang Shouting Wenbin Song 1 Xiaoyu Lu Xiaoying Lan Xin Chen Songgang Yi Xu 1,3 Lili Jiang Canguo Xiaoxian Dong Ping Zhou Shujue 1,4 Shunqing Wang Xianping Shi Q. Dou 1,5 Xuejun 1,6 and Jinbao State Key Lab of Respiratory Disease, Protein Modification Degradation Lab, Department Pathophysiology, Guangzhou Medical University, Guangdong, China 2 Research Institute Cardiovascular the Second Affiliated Hospital,...
Abstract Oxidative DNA damage plays crucial roles in the pathogenesis of numerous diseases including cancer. 8-hydroxy-2′-deoxyguanosine (8-OHdG) is most representative product oxidative modifications DNA, and urinary 8-OHdG potentially best non-invasive biomarker to DNA. Herein, we developed a sensitive, specific accurate method for quantification human urine. The urine samples were pretreated using off-line solid-phase extraction (SPE), followed by ultrahigh performance liquid...
L-carnitine (LC) is generally believed to transport long-chain acyl groups from fatty acids into the mitochondrial matrix for ATP generation via citric acid cycle. Based on Warburg's theory that most cancer cells mainly depend glycolysis generation, we hypothesize that, LC treatment would lead disturbance of cellular metabolism and cytotoxicity in cells. In this study, Human hepatoma HepG2, SMMC-7721 cell lines, primary cultured thymocytes mice bearing HepG2 tumor were used. content was...
Gambogic acid (GA) is a natural compound derived from Chinese herbs that has been approved by the Food and Drug Administration for clinical trials in cancer patients; however, its molecular targets have not thoroughly studied. Here, we report GA inhibits tumor proteasome activity, with potency comparable to bortezomib but much less toxicity. First, acts as prodrug only gains proteasome-inhibitory function after being metabolized intracellular CYP2E1. Second, GA-induced inhibition...
Abstract The successful development of bortezomib-based therapy for treatment multiple myeloma has established proteasome inhibition as an effective therapeutic strategy and both 20S peptidases 19S deubiquitinases (DUBs) are becoming attractive targets cancer therapy. It been reported that metal complexes, such copper inhibit tumor proteasome. However, the involved mechanism action not fully characterized. Here we report (i) pyrithione (CuPT), alternative to tributyltin antifouling paint...
Chloride channel accessory 1 (CLCA1) belongs to the calcium-sensitive chloride conductance protein family, which is mainly expressed in colon, small intestine and appendix. This study was conducted investigate functions mechanisms of CLCA1 colorectal cancer (CRC). The expression level CRC patients evaluated by enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), western blotting analysis. Using CRISPR/Cas9 technology, CLCA1-upregulated (CLCA1-ACT) CLCA1-knockout cells...
Feeding requires sophisticated orchestration of neural processes to satiate appetite in natural, capricious settings. However, the complementary roles discrete populations orchestrating distinct behaviors and motivations throughout feeding process are largely unknown. Here, we delineate behavioral repertoire mice by developing a machine-learning-assisted behavior tracking system show that is fragmented divergent for food consumption or environment exploration compete process. An iterative...
// Hongbiao Huang 1, * , Yuning Liao Ningning Liu 2 Xianliang Hua 1 Jianyu Cai Changshan Yang Huidan Long Chong Zhao Xin Chen Xiaoying Lan Dan Zang Jinjie Wu Xiaofen Li Xianping Shi Xuejun Wang 3 Jinbao State Key Laboratory of Respiratory Disease, Protein Modification and Degradation Laboratory, Department Pathophysiology, Guangzhou Medical University, Guangdong 511436, People’s Republic China Research Institute Cardiovascular The Second Affiliated Hospital, 510260, Division Basic...
Immune checkpoint inhibitors are increasingly being utilized for the treatment of advanced neoplastic disease, and Sintilimab as a selective anti–PD-1 antibody that inhibits interactions between PD-1 its ligand, is typical representative them. Among all adverse effects(AEs) sintilimab, skin AEs had affected many people. Though exceedingly rare, eruptive keratoacanthomas-like lesion have been associated with use immune before. Here, we report case numerous keratoacanthoma-like lesions arising...
Recently, we have reported a series of new 1,3-symmetrically (R 1 = R 3) substituted xanthines ( 3 and 4) which high affinity selectivity for the human adenosine A 2B receptors (hA(2B)-AdoR). Unfortunately, this class compounds had poor pharmacokinetic properties. This prompted us to investigate effect differential alkyl substitution at N-1 N-3 positions N 1-R not equal 3-R) on A(2B)-AdoR selectivity; dual objectives enhancing A(2B)-AdoR, as well improving oral bioavailability. effort has...
DNA is the well-known molecular target of current platinum-based anticancer drugs; consequently, their clinical use severely restricted by systemic toxicities and drug resistance originating from non-selective damage. Various strategies have been developed to circumvent shortcomings chemotherapy but inherent problem remains unsolved. Here we report that platinum pyrithione (PtPT), a chemically well-characterized synthetic complex platinum, inhibits proteasome function thereby exhibits...
Proteins, as executives of genes' instructions, are responsible for cellular phenotypes. Integratingproteomics with gene microarray, we conducted this study to identify potential protein biomarkers colorectal cancer (CRC). Isobaric tags related and absolute quantitation (iTRAQ) labeling mass spectrometry (MS) was applied screen differentially expressed proteins between paired CRC adjacent normal mucosa. Meanwhile, Affymetrix U133plus2.0 microarrays were used perform microarray analysis....
Significance The mechanotransduction (MT) complex in auditory hair cells converts the mechanical stimulation of sound waves into neural signals. Our findings reveal previously unrecognized physical and functional interactions TMC1 LHFPL5, two critical components MT complex, provide insights molecular mechanism by which D572N mutation causes deafness. also identify a missing link currently known organization micromolecular complex. Furthermore, demonstrating power microbead-based SiMPull...
The channel that governs mechanotransduction (MT) by hair cells in the inner ear has been investigated intensively for 4 decades, but its precise molecular composition remains enigmatic. Transmembrane channel-like protein 1 (TMC1) was recently identified as a component of MT channel, and lipoma HMGIC fusion partner-like 5 (LHFPL5) is considered to be part complex may functionally couple tip link channel. As components complex, TMC1 LHFPL5 are expected localize at lower end cells, notion...
The ubiquitin-proteasome system (UPS) plays a central role in various cellular processes through selectively degrading proteins involved critical functions. Targeting UPS has been validated as novel strategy for treating human cancer, inhibitors of the 20S proteasome catalytic activity are currently clinical use treatment multiple myeloma and other cancers, deubiquitinase associated with is also valid target anticancer agents. Recent studies suggested that zinc pyrithione, an FDA-approved...
Pseudomonas aeruginosa (P. aeruginosa) is a known bacterium that produces biofilms and causes severe infection. Furthermore, P. are extremely difficult to eradicate, leading the development of chronic antibiotic-resistant infections. Our previous study showed cathelicidin-related antimicrobial peptide (CRAMP) inhibits formation markedly reduces biomass preformed biofilms, while mechanism eradicating bacterial remains elusive. Therefore, in this study, potential by which CRAMP eradicates was...
Abstract Triple-negative breast cancer (TNBC) is a heterogeneous malignancy in women. It associated with poor prognosis, aggressive malignant behavior, and limited treatment options. In the ubiquitin‒proteasome system (UPS), deubiquitinases (DUBs) are potential therapeutic targets for various tumors. this study, by performing unbiased siRNA screening, we identified STAMBP, JAMM metalloprotease DUB family, as driver of human TNBC tumor growth. Functionally, knockdown STAMBP inhibited...
Combinations of proteasome inhibitors and histone deacetylases (HDAC) appear to be the most potent produce synergistic cytotoxicity in preclinical trials. We have recently confirmed that L-carnitine (LC) is an endogenous HDAC inhibitor. In current study, anti-tumor effect LC plus inhibitor bortezomib (velcade, Vel) was investigated both cultured hepatoma cancer cells Balb/c mice bearing HepG2 tumor. Cell death cell viability were assayed by flow cytometry MTS, respectively. Gene, mRNA...