A5072458329- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Molecular Biology Techniques and Applications
- CRISPR and Genetic Engineering
- BRCA gene mutations in cancer
- Effects of Radiation Exposure
- Microtubule and mitosis dynamics
- Aortic Thrombus and Embolism
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Food Security and Health in Diverse Populations
- Cardiac Imaging and Diagnostics
- Cell death mechanisms and regulation
- Sarcoma Diagnosis and Treatment
- Advanced Proteomics Techniques and Applications
- Hepatitis Viruses Studies and Epidemiology
- Cardiac tumors and thrombi
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Galectins and Cancer Biology
- Diabetes Management and Research
- Public Health Policies and Education
Hennepin County Medical Center
2021-2024
Hennepin Healthcare Research Institute
2021-2023
Hennepin County
2023
University of Minnesota
2014-2021
University of Minnesota Medical Center
2020-2021
Masonic Cancer Center
2020
Hormel (United States)
2014-2017
Mayo Clinic Health System
2017
Immunoblotting, after polyacrylamide gel electrophoresis with sodium dodecyl sulfate (SDS-PAGE), is a technique commonly used to detect specific proteins. SDS-PAGE often results in the visualization of protein band(s) addition one expected based on theoretical molecular mass (TMM) interest. To determine likelihood additional being nonspecific, we liquid chromatography - spectrometry identify proteins that were extracted from bands apparent (MM) 40 and 26 kD, originating extracts derived...
Sporadic carcinogenesis starts from immortalization of a differentiated somatic cell or an organ-specific stem cell. The immortalized incepts new quasinew organism that lives like parasite in the patient and usually proceeds to progressive simplification, constantly engendering intermediate organisms are simpler than normal cells. Like organismal evolution Mother Nature, this cellular simplification is process Darwinian selection those mutations with growth- survival-advantages, numerous...
Bone mass loss (osteoporosis) seen in postmenopausal women is an adverse factor for implant denture. Using ovariectomized rat model, we studied the mechanism of estrogen-deficiency-caused bone and therapeutic effect Zoledronic acid. We observed that ovariectomized-caused resorption tissue mandible was evident at four weeks had not fully recovered by 12 post-ovariectomized compared with sham-operated controls. Further evaluation a TUNEL assay showed enhanced apoptosis osteoblasts but...
To determine the impact of adverse social and behavioral determinants health (SBDH) on care use in a safety-net community hospital (SNCH) heart failure (HF) population.
Hennepin County Adult Detention Center (Jail) is Minnesota's largest jail. In August 2019, the Minnesota Department of Health declared a statewide hepatitis A outbreak. Within three days, Jail Services made significant changes to vaccination protocols that increased rates from 0.6% 7.1% among detainees, who have greater risk contracting A. We highlight opportunity for jails develop sustainable public health interventions in setting community outbreaks.
The small nucleolar RNA host gene 16 (SNHG16) has recently been shown to be a putative oncogene in gastric cancer (GC) and other types, but how its four lncRNA variants are expressed any physiological pathological situation remains unknown. To investigate the expression function of SNHG16, mainly variant 1, GC, we performed quantitative PCR determine levels 60 GC tissue samples several cell lines. We also studied knocking down SNHG16 with siRNA affected proliferation, apoptosis, cycle...
// Rui Yan 1 , Ju Zhang 2 Lucas Zellmer 3 Lichan Chen Di Wu 4 Siqi Liu Ningzhi Xu 5 and Joshua D. Liao 6 Nephrology Department, Guizhou Medical University Hospital, Guiyang, P.R. China CAS Key Laboratory of Genome Sciences Information, Beijing Institute Genomics, Chinese Academy Sciences, Beijing, Hormel Institute, Minnesota, Austin, USA Protein Innovation Co., Ltd, Cell Molecular Biology & State Oncology, National Cancer Center/Cancer Peking Union College, Department Pathology,...
Background Previous studies revealed the connection between left atrial appendage peak flow velocity (LAA-PEV) and postoperative persistent fibrillation (AF) recurrence. Yet, this association is not necessarily generalizable to AF patients undergoing initial cryoballoon ablation due current gaps in literature. Methods We prospectively studied 74 consecutive individuals with a for first time January 2018 2020. Before ablation, LAA-PEV was documented by transesophageal echocardiography (TEE)....
Most genes in evolutionarily complex genomes are expressed to multiple protein isoforms, but there is not yet any simple high-throughput approach identify these isoforms. Using an oversimplified top-down LC-MS/MS strategy, we detected, around the 26-kD position of SDS-PAGE, proteins produced from 782 a Cdk4-/- mouse embryonic fibroblast cell line. Interestingly, only 213 (27.24%, about one-fourth) have their with theoretical molecular mass (TMM) 10% smaller or larger than 26 kD, that is,...
We performed polyacrylamide gel electrophoresis of human proteins with sodium dodecyl sulfate, isolated at multiple positions, and then used liquid chromatography tandem mass spectrometry (LC-MS/MS) to determine the protein identities. Although beta-actin (ACTB) glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are 41.7 36 kDa proteins, respectively, LC-MS/MS identified their peptides all positions studied. The National Center for Biotechnology Information (USA) database lists only one ACTB...
We determined RNA spectrum of the human RSK4 (hRSK4) gene (also called RPS6KA6) and identified 29 novel mRNA variants derived from alternative splicing, which, plus NCBI-documented ones five we reported previously, totaled 50 hRSK4 RNAs that, by our bioinformatics analyses, encode 35 protein isoforms 35–762 amino acids. Many mRNAs are bicistronic or tricistronic for hRSK4. The NCBI-normalized NM_014496.5 it encodes designated herein as Wt-1 protein, respectively, whereas NM_001330512.1 long...
Introduction: Higher values of valvulo-arterial impedance (Zva), a marker global hemodynamic load, are associated with worse outcomes in patients aortic stenosis (AS). No studies have evaluated the impact Zva on end stage kidney disease (ESKD) AS. Objectives We aim to evaluate clinical and echocardiographic correlates AS ESKD. Methods: Consecutive ESKD dialysis echocardiography from single center between January 2000 March 2021. severity was defined using AHA guideline recommendations....
Heat shock proteins (HSP) serve as chaperones to maintain the physiological conformation and function of numerous cellular when ambient temperature is increased. To determine how accurate general assumption that HSP gene expression increased in febrile situations is, RNA levels HSF1 (heat transcription factor 1) certain genes were determined three cell lines cultured at 37˚C or 39˚C for days. At 39˚C, HSF1, HSPB1, HSP90AA1 HSP70A1L demonstrated complex changes ratios between different...
Abstract Background Patients with a history of cardiac disease are prone to develop cardiovascular adverse events such as hypotension, hypertension, and tachycardia during anesthesia induction. Therefore, hemodynamic stability is one the most important concerns for induction in patients undergoing surgery. Remimazolam tosilate new, ultra-short-acting benzodiazepine agent, advantages rapid onset, offset, minimal cardiorespiratory depression. We aim compare effect remimazolam etomidate on...