A5101766266- Salivary Gland Disorders and Functions
- Liver Diseases and Immunity
- Liver Disease Diagnosis and Treatment
- Systemic Lupus Erythematosus Research
- Rheumatoid Arthritis Research and Therapies
- Immune Cell Function and Interaction
- Diabetes and associated disorders
- Chemokine receptors and signaling
- Immunotherapy and Immune Responses
- Cancer-related molecular mechanisms research
- Spondyloarthritis Studies and Treatments
- Systemic Sclerosis and Related Diseases
- Galectins and Cancer Biology
- Metabolism, Diabetes, and Cancer
- Inflammatory Myopathies and Dermatomyositis
- Inflammatory Bowel Disease
- Celiac Disease Research and Management
- Health, Environment, Cognitive Aging
- Urticaria and Related Conditions
- Tuberculosis Research and Epidemiology
- Diagnosis and treatment of tuberculosis
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Lymphatic System and Diseases
- Cutaneous lymphoproliferative disorders research
- Microscopic Colitis
Peking Union Medical College Hospital
2014-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2024
China-Japan Friendship Hospital
2023
National Clinical Research Center for Digestive Diseases
2022
Ministry of Education of the People's Republic of China
2022
Academy of Medical Sciences
2012
Epigenetics, especially DNA methylation, plays an important role in the pathogenesis of primary Sjogren syndrome (pSS). Our study aimed to reveal methylation peripheral monocytes pSS patients.A total 11 patients and five age-matched healthy controls (HCs) were included this study. Monocytes isolated from blood mononuclear cells using magnetic microbeads. profiles generated Human Methylation 850K BeadChips.In patients, we identified 2819 differentially methylated positions (DMPs), comprising...
Objective The pathogenesis of primary Sjögren’s syndrome (pSS) remains incompletely understood. N6-methyladenosine (m6A) RNA modification, the most abundant internal transcript has close associations with multiple diseases. This study aimed to investigate role m6A in patients pSS. Materials and methods enrolled 44 pSS, 50 age- gender-matched healthy controls (HCs), 11 non-SS sicca. We detected messenger (mRNA) levels elements (including METTL3, WTAP, RBM15, ALKBH5, FTO, YTHDF1, YTHDF2,...
The relationship between the cluster of differentiation 226 (CD226)/T cell Ig and ITIM domain (TIGIT) immune checkpoint primary biliary cholangitis (PBC) pathogenesis is unknown. Herein, PBC patients (n=42) showed significantly higher proportions peripheral CD8+ T CD4+ cells expressing either CD226 or TIGIT than disease (n=25) healthy (n=30) controls. percentage CD8+TIGIT+ was negatively associated with total bilirubin, direct bile acid, γ-glutamyl transpeptidase, alkaline phosphatase, but...
Primary Sjögren's syndrome (pSS) is a chronic systemic autoimmune disease characterized by B cell hyperactivation and hypergrammaglobulinemia. Currently, the role of metabolic pathways in cells pSS patients poorly defined. Here, we showed that upon cytosine phosphate-guanine (CpG)/sCD40L/IL-4 stimulation, proportionally increased glycolysis oxygen consumption, compared with from healthy controls (HCs), exhibited higher capacity maximal oxidative respiration (OXPHOS). We also found glucose...
Abstract Primary Sjögren’s syndrome (pSS) is a prevalent autoimmune disorder wherein CD4 + T cells play pivotal role in its pathogenesis. However, the underlying mechanisms driving hyperactivity of pSS remain poorly understood. This study aimed to investigate potential immunometabolic alterations pSS. We employed Seahorse XF assay evaluate metabolic phenotype cells, conducted flow cytometry assess effector function and differentiation measured level intracellular reactive oxygen species...
Background Neuromyelitis optica spectrum disorder (NMOSD) is a neurological complication of primary Sjögren's syndrome (pSS). Objective We aimed to explore potential serological differences between pSS patients with and without NMOSD. Methods There were 4 NMOSD 8 enrolled as the screening group for two-dimensional difference gel electrophoresis (DIGE) analysis. Then differential expressed protein spots groups identified by MALDI-TOF/TOF MS. The levels biomarkers verified ELISA in second...
The need for reliable biomarkers distinguishing Crohn disease (CD) from ulcerative colitis (UC) is increasing. This study aimed at evaluating the diagnostic potential of anti-GP2 antibodies as a biomarker in Chinese patients with CD. In addition, variety autoantibodies, including anti-saccharomyces cerevisiae (ASCA), perinuclear anti-neutrophil cytoplasmic (PANCA), anti-intestinal goblet cell autoantibodies (GAB), and anti-pancreatic (PAB), were evaluated. A total 91 subjects prospectively...
Here, we investigated the pathogenesis of primary biliary cirrhosis (PBC) by using 2D-DIGE to analyze serological differences between anti-mitochondrial antibody (AMA)-positive and -negative PBC patients. The study comprised 30 patients with PBC; 20 AMA-positive ten AMA-negative matched for age, sex, pathological stage. A screening group (four four patients) was used 2D-DIGE. Protein spots that were differently abundant two groups identified via dye intensity MS. Nine candidate proteins from...
Objectives: S100 calcium binding protein A12 (S100A12) has been supposed to be a pro-inflammatory factor associated with non-infectious inflammatory diseases. However, whether S100A12 is involved in the process of primary biliary cirrhosis (PBC) not shown.Methods: The levels mRNA transcripts peripheral mononuclear blood cells (PBMCs) 66 Chinese patients (PBC), 62 healthy controls (HC) and 55 chronic hepatitis B (CHB) were measured by qRT-PCR. serum concentrations 34 PBC ELISA.Results: PBMCs...
The CD155-CD226/T-cell Ig and immunoreceptor tyrosine-based inhibitory motif (ITIM) domain (TIGIT) pathway plays a critical role in regulating T-cell responses is being targeted clinically. However, research on the of this autoimmune diseases limited. This study aimed to investigate expression tissue-specific roles CD155-CD226/TIGIT molecules inflamed muscles patients with idiopathic inflammatory myopathies (IIMs).Immunohistochemistry, Western blot analysis, polychromatic immunofluorescence...
To investigate the prevalence of GAD antibody (GADA) in general adult population and to evaluate its predictive value for diabetes China.We searched PUMCH-HM database identified 36,731 subjects with GADA test results from 2012 2015. We then established a retrospective cohort 4835 nondiabetic at baseline complete annual health evaluation records through 2019. The median follow-up time was 4.8 (3.0-7.3) years.The overall 0.53% higher diabetic (1.25%) than (0.47%). found decrease body mass...
Abstract Background The aim of this study was to elucidate the expression profile and potential role long non-coding RNA (LncRNA) in peripheral blood mononuclear cells primary Sjögren’s syndrome (pSS) patients. Methods RNA-seq technology used detect differentially expressed LncRNAs mRNAs between five paired pSS patients healthy control PBMCs. selected were detected validation by RT-qPCR 16 controls. GO, KEGG, co-localization, co-expression analysis performed enrich gene functions pathways....