A5100444421- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- Cell Adhesion Molecules Research
- Cytokine Signaling Pathways and Interactions
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- interferon and immune responses
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Wnt/β-catenin signaling in development and cancer
- Inflammasome and immune disorders
- Ferroptosis and cancer prognosis
- Salivary Gland Disorders and Functions
- Pregnancy and preeclampsia studies
- Immune Cell Function and Interaction
- Immune cells in cancer
- T-cell and B-cell Immunology
- Angiogenesis and VEGF in Cancer
- Reproductive System and Pregnancy
- Kruppel-like factors research
- Axon Guidance and Neuronal Signaling
- Iron oxide chemistry and applications
- Macrophage Migration Inhibitory Factor
Guangzhou Medical University
2024-2025
South China University of Technology
2024
Anhui University
2023-2024
Central South University
2020-2024
Chinese National Engineering Research Center for Control and Treatment of Heavy Metal Pollution
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2020-2024
Jinzhou Medical University
2017-2024
Shanghai University
2022-2023
University of Science and Technology of China
2019-2023
Wannan Medical College
2023
Abstract The availability of high-quality RNA-sequencing and genotyping data post-mortem brain collections from consortia such as CommonMind Consortium (CMC) the Accelerating Medicines Partnership for Alzheimer’s Disease (AMP-AD) enable generation a large-scale cis- eQTL meta-analysis. Here we generate cerebral cortical 1433 samples available four cohorts (identifying >4.1 million significant >18,000 genes), well cerebellar 261 874,836 >10,000 genes). We find substantially improved...
Abstract Several studies show that the immunosuppressive drugs targeting interleukin-6 (IL-6) receptor, including tocilizumab, ameliorate lethal inflammatory responses in COVID-19 patients infected with SARS-CoV-2. Here, by employing single-cell analysis of immune cell composition two severe-stage prior to and following tocilizumab-induced remission, we identify a monocyte subpopulation contributes cytokine storms. Furthermore, although tocilizumab treatment attenuates inflammation, cells,...
Abstract Maintaining homeostasis of the decidual immune microenvironment at maternal–fetal interface is essential for placentation and reproductive success. Although distinct cell subpopulations have been identified under normal conditions, systematic understanding spectrum heterogeneity leukocytes recurrent miscarriage in human deciduas remains unclear. To address this, we profiled respective transcriptomes 18,646 primary cells isolated from patients with pregnancy loss (RPL) healthy...
A central question in Wnt signaling is the regulation of beta-catenin phosphorylation and degradation. Multiple kinases, including CKI alpha GSK3, are involved phosphorylation. Protein phosphatases such as PP2A PP1 have been implicated beta-catenin. However, which phosphatase dephosphorylates vivo how specificity dephosphorylation regulated not clear. In this study, we show that regulates degradation vivo. We demonstrate required for Wnt/beta-catenin Drosophila. Moreover, identified PR55...
Wnt signaling plays important roles in development and tumorigenesis. A central question about the pathway is regulation of β-catenin. Phosphorylation β-catenin by CK1α GSK3 promotes binding to β-TrCP, leading degradation through proteasome. The phosphorylation ubiquitination have been well characterized; however, it unknown whether how a deubiquitinase involved. In this study, screening RNA interference (RNAi) libraries, we identified USP47 as that prevents ubiquitination. Inactivation RNAi...
Genome-wide association studies indicate that many disease susceptibility regions reside in non-protein-coding of the genome. Long noncoding RNAs (lncRNAs) are a major component genome, but their biological impacts not fully understood. Here, we performed CRISPR interference (CRISPRi) screen on 2263 epidermis-expressed lncRNAs and identified nine novel candidate regulating keratinocyte proliferation. We further characterized top hit from screen, progenitor renewal associated non-coding RNA (...
Epigenetic and transcriptomic profiling of developing human retina retinal organoids revealed regulators in development.
We aimed to explore the expression level and biological function of lncRNA PVT1 in human trophoblast cells.The levels cancer cell lines, HTR8/SVneo cell, HUVEC maternal placenta GDM patients, PE patients normal pregnancy were detected by qRT-PCR. The culture, transfection, CCK-8 assay, flow cytometry, wound scratch assay transwell carried out determine effects silencing overexpression on line. Nuclear chromatin RNA fraction RNA-sequencing, western blot qRT-PCR conducted preliminarily...
Krüppel-like factor 4 (KLF4) is a zinc finger transcription that plays vital role in regulating cell lineage differentiation during development and maintaining epithelial homeostasis the intestine. In normal intestine, KLF4 predominantly expressed differentiated cells. It has been identified as tumor suppressor colorectal cancer. knockout mice demonstrated decrease number of goblet cells colon, conditional ablation from intestinal epithelium led to altered homeostasis. However, colon cancer...
Abstract The NLRP3 inflammasome regulates innate immune and inflammatory responses by promoting caspase1‐dependent induction of pro‐inflammatory cytokines. However, aberrant activation causes diverse diseases, thus activity must be tightly controlled. Here, we reveal a molecular mechanism underlying the regulation inflammasome. interacts with SUMO‐conjugating enzyme (UBC9), which subsequently promotes small ubiquitin‐like modifier 1 (SUMO1) to catalyze SUMOylation at residue Lys 204 ....
Long noncoding RNA (lncRNA) growth arrest-specific transcript 5 (GAS5) has been revealed to be associated with the progression of various cancers. However, biological roles GAS5 in esophageal cancer (EC) remain unclear. We aimed thoroughly explore functions EC. The results showed that expression was increased EC cells (ECA109, TE-1, TE-3, and EC9706) compared SHEE cells. Knockdown decreased cell viability, migration, invasion induced apoptosis EC9706 Moreover, miR-301a appeared directly...
Abstract Systemic sclerosis (SSc) is a disease at the intersection of autoimmunity and fibrosis. However, epigenetic regulation contributions diverse cell types to SSc remain unclear. Here we survey, using ATAC-seq, active DNA regulatory elements eight primary cells in normal skin from healthy controls, as well clinically affected unaffected patients. We find that accessible skin-resident dendritic (DCs) exhibit highest enrichment SSc-associated single-nucleotide polymorphisms (SNPs) predict...
Although mitotic chromosomes are highly compacted and transcriptionally inert, some active chromatin features retained during mitosis to ensure the proper postmitotic reestablishment of maternal transcriptional programs, a phenomenon termed "mitotic bookmarking." However, dynamics regulation bookmarking have not been systemically surveyed. Using single-cell transposase-accessible sequencing (scATAC-seq), we examined 6538 L02 human liver cells variable stages found that accessibility remained...
Asxl1 loss–induced myeloid malignancies are mediated partially through impaired ASXL1 cohesin interaction and functions.
Background: To explore the association between clinical phenotype of hyperthyroidism in children and exon gene mutation locus children. Methods: This study collected data from 39 with at Guangzhou Women Children’s Medical Center 2023. Apriori rules are used to analyze locus. Results: It is discovered that girls associated mutations TG, LHCGR, TSHR other genes. Hyperthyroidism often occurs school age, age patients related When there no family history hyperthyroidism, mostly GNAS CTLA4...
We compared pairs of 1,226 bacterial strains with whole genome sequences and calculated their average nucleotide identity (ANI) between genomes to determine whether comparison can be directly used for species definition. found that comparisons two from the same (SGC) have a significantly higher ANI than those different (DGC), query reference come species. Bacterial definition based on cut-off value 0.92 matched well (81.5%) current The was shown consistent standard traditional definition, it...
Dendritic cells are the primary antigen-presenting that contact trophoblasts at beginning of pregnancy. Excessive DCs maturity is described in some pregnancy complications, such as pre-eclampsia and fetal growth restriction, which characterized by impaired trophoblast invasion. However, mechanism unclear. The long non-coding RNA DC (lnc-DC) expressed exclusively conventional human induces differentiation maturation promoting signal transducer activator transcription 3 (STAT3)...
Abstract The aim of this study was to elucidate the expression profile and potential role long non-coding RNA (LncRNA) in peripheral blood mononuclear cells primary Sjögren’s syndrome (pSS) patients. RNA-seq technology used detect differentially expressed LncRNAs mRNAs between five age-and sex-matched paired pSS patients healthy control PBMCs. selected were detected validation by RT-qPCR 16 controls. GO, KEGG, co-localization, co-expression analysis performed enrich gene functions pathways....
Cholangiocarcinoma (CCA) development is an extremely complex process with alterations occurring in numerous genes.SNHG6, a validated lncRNA, has been reported to regulate the expression of multiple tumor-related genes hepatocellular carcinoma, colorectal cancer and breast cancer.Here, we elucidated function possible molecular mechanisms SNHG6 human CCA cells.Our results proved that was upregulated tissues cell lines.Ectopic promoted proliferation, cycle progression, migration, angiogenesis...