A5089304985- Polyomavirus and related diseases
- Bacteriophages and microbial interactions
- Psoriasis: Treatment and Pathogenesis
- Full-Duplex Wireless Communications
- Dermatology and Skin Diseases
- Antenna Design and Analysis
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Plant Virus Research Studies
- Inflammatory Myopathies and Dermatomyositis
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Cutaneous lymphoproliferative disorders research
- Eosinophilic Disorders and Syndromes
- Systemic Sclerosis and Related Diseases
- Autoimmune Bullous Skin Diseases
- Chemotherapy-related skin toxicity
- Melanoma and MAPK Pathways
- Radiomics and Machine Learning in Medical Imaging
- Drug-Induced Adverse Reactions
- Allergic Rhinitis and Sensitization
- Nonmelanoma Skin Cancer Studies
- CNS Lymphoma Diagnosis and Treatment
- Immune cells in cancer
Palo Alto University
2020-2024
Stanford University
2012-2024
Stanford Medicine
2020-2023
RELX Group (United States)
2023
Stanford Health Care
2021
VA Palo Alto Health Care System
2021
Palo Alto Institute
2021
University Dermatology
2020
Howard Hughes Medical Institute
2013-2015
Rockefeller University
2007-2014
Background Psoriasis vulgaris is an inflammatory skin disease mediated by Th1 and Th17 cytokines, yet the relative contribution of interferon (IFN)‐γ, interleukin (IL)‐17 IL‐22 on pathogenesis still unknown. Objectives In this study, we sought to identify cytokines produced skin‐resident T cells in normal skin, localize receptors for these examine how alter gene expression profiles bearing cognate receptors. Methods We used intracellular cytokine staining flow cytometry evaluate cell...
Biological agents have dramatically improved treatment options for patients with severe psoriasis. Etanercept (tumor necrosis factor [TNF] receptor-immunoglobulin fusion protein) is an effective many psoriasis patients, and blockade of TNF considered to be its primary action. However, in this clinical trial, we show that etanercept has early inhibitory effects on a newly appreciated type T cells: helper 17 (Th17) cells. reduced the inflammatory dendritic cell products drive Th17...
A genome-wide association study was performed to identify genetic factors involved in susceptibility psoriasis (PS) and psoriatic arthritis (PSA), inflammatory diseases of the skin joints humans. 223 PS cases (including 91 with PSA) were genotyped 311,398 single nucleotide polymorphisms (SNPs), results compared those from 519 Northern European controls. Replications an independent cohort 577 737 controls U.S., 576 PSA patients 480 U.K.. Strongest associations class I region major...
Objective Since dermatomyositis (DM) is associated with an increased risk of malignancy, accurate identification patients likely to harbor cancers important. Using immunoprecipitations from radiolabeled cell lysates, several groups recently showed that anti–transcription intermediary factor 1γ (anti–TIF‐1γ) antibodies are malignancy in DM. We undertook this study develop sensitive, specific assays detect against TIF‐1γ and nuclear matrix protein NXP‐2 evaluate their association Methods To...
The classical Th1/Th2 paradigm previously defining atopic dermatitis (AD) and psoriasis has recently been challenged with the discovery of Th17 T cells that synthesize IL-17 IL-22. Although it is becoming evident many Th1 diseases including have a strong signal, importance in AD still unclear. We examined compared skin biopsies from patients by gene microarray, RT-PCR, immunohistochemistry, immunofluorescence. found reduced genomic expression IL-23, IL-17, IFN-gamma psoriasis. To define...
We used a panel of monoclonal antibodies to characterize DCs in the dermis normal human skin. Staining for CD11c integrin, which is abundant on many kinds DCs, revealed cells upper dermis. These were positive blood DC antigen–1 (BDCA-1; also known as CD1c), HLA-DR, and CD45, markers that are expressed by circulating myeloid DCs. A small subset CD11c+ dermal DEC-205/CD205 DC-lysosomal–associated membrane glycoprotein/CD208 (DC-LAMP/CD208), suggesting some differentiation or maturation. When...
Background Our objective was to develop a consistent molecular definition of psoriasis. There have been several published microarray studies psoriasis, and we compared disease-related genes identified across these different psoriasis with our own in order establish consensus. Methodology/Principal Findings We present transcriptome from group 15 patients enrolled clinical study, assessed its biological validity using set important pathways known be involved also key cytokines that are now...
Abstract Therapeutic modulation of psoriasis with targeted immunosuppressive agents defines inflammatory genes associated disease activity and may be extrapolated to a wide range autoimmune diseases. Cyclosporine A (CSA) is considered “gold standard” therapy for moderate-to-severe psoriasis. We conducted clinical trial CSA analyzed the treatment outcome in blood skin 11 responding patients. In skin, as expected, modulated from activated T cells “type 1” pathway (p40, IFN-γ, STAT-1-regulated...
Understanding the immune responses to SARS-CoV-2 vaccination is critical optimizing strategies for individuals with autoimmune diseases, such as systemic lupus erythematosus (SLE). Here, we comprehensively analyzed innate and adaptive in 19 patients SLE receiving a complete 2-dose Pfizer-BioNTech mRNA vaccine (BNT162b2) regimen compared control cohort of 56 healthy (HC) volunteers. Patients exhibited impaired neutralizing antibody production antigen-specific CD4+ CD8+ T cell relative HC....
Mutations in the caspase recruitment domain, family member 14 (CARD14) gene have recently been described psoriasis patients, and explain susceptibility locus 2 (PSORS2). CARD14 is a scaffolding protein that regulates NF-κB activation, psoriasis-associated mutations lead to enhanced signaling. expressed mainly epidermal keratinocytes, but also unidentified dermal cells. In this manuscript, identity of cell types expressing CARD14, as well potential functional consequence overactive these...
Abstract Systemic sclerosis (SSc) is a disease at the intersection of autoimmunity and fibrosis. However, epigenetic regulation contributions diverse cell types to SSc remain unclear. Here we survey, using ATAC-seq, active DNA regulatory elements eight primary cells in normal skin from healthy controls, as well clinically affected unaffected patients. We find that accessible skin-resident dendritic (DCs) exhibit highest enrichment SSc-associated single-nucleotide polymorphisms (SNPs) predict...