A5049216072- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Long-Term Effects of COVID-19
- SARS-CoV-2 detection and testing
- Genomics and Phylogenetic Studies
- Glycosylation and Glycoproteins Research
- Respiratory viral infections research
- Inflammasome and immune disorders
- interferon and immune responses
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Transgenic Plants and Applications
- Oral microbiology and periodontitis research
- Cell death mechanisms and regulation
- Viral Infections and Immunology Research
- Drug-Induced Adverse Reactions
- Intestinal and Peritoneal Adhesions
- COVID-19 diagnosis using AI
- Innovations in Medical Education
- Escherichia coli research studies
- Animal Virus Infections Studies
- Microbial Natural Products and Biosynthesis
- Food Allergy and Anaphylaxis Research
- vaccines and immunoinformatics approaches
Chinese University of Hong Kong, Shenzhen
2023-2024
Zhejiang Chinese Medical University
2024
University of Science and Technology of China
2019-2023
Nanjing University of Finance and Economics
2023
Guangdong Academy of Medical Sciences
2020-2023
Guangdong Provincial People's Hospital
2020-2023
Southern Medical University
2023
Anhui Medical University
2016-2023
First Affiliated Hospital of Anhui Medical University
2023
Hefei National Center for Physical Sciences at Nanoscale
2019-2022
Currently, detecting SARS-CoV-2 RNAs is a standard approach for COVID-19 diagnosis.However, there an urgent need reliable and rapid serological diagnostic methods to screen SARS-CoV-2infected people including those who do not have overt symptoms.][3][4] Although detection of SARS-CoV-2-specific IgA in serum has been reported few papers, 5,6 analyses levels larger number patients are still lacking.
Abstract Several studies show that the immunosuppressive drugs targeting interleukin-6 (IL-6) receptor, including tocilizumab, ameliorate lethal inflammatory responses in COVID-19 patients infected with SARS-CoV-2. Here, by employing single-cell analysis of immune cell composition two severe-stage prior to and following tocilizumab-induced remission, we identify a monocyte subpopulation contributes cytokine storms. Furthermore, although tocilizumab treatment attenuates inflammation, cells,...
Abstract Background The pandemic of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is causing great loss. Detecting viral RNAs standard approach for SARS-CoV-2 diagnosis with variable success. Currently, studies describing serological diagnostic methods are emerging, while most them just involve detection SARS-CoV-2-specific IgM and IgG by ELISA or “flow immunoassay” limited accuracy. Methods Diagnostic depends on chemiluminescence immunoanalysis (CLIA) detecting IgA,...
To date, SARS-CoV-2 has caused tremendous loss of human life and economic output worldwide. Although a few COVID-19 vaccines have been approved in several countries, the development effective therapeutics, including targeting antibodies, remains critical.
The spread of Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), resulting in a global pandemic with around four million deaths. Although there are variety nucleic acid-based tests for detecting SARS-CoV-2, these methods have relatively high cost and require expensive supporting equipment. To overcome limitations improve the efficiency SARS-CoV-2 diagnosis, we developed microfluidic platform that collected serum pulling-force...
ABSTRACT Despite the current devastation of COVID-19 pandemic, several recent studies have suggested that immunosuppressive drug Tocilizumab can powerfully treating inflammatory responses occur in this disease. Here, by employing single-cell analysis immune cell composition severe-stage patients and these same post Tocilizumab-treatment remission, we identified a monocyte subpopulation specific to severe disease contributes storms patients. Although treatment attenuated strong response,...
SARS-CoV-2 variants with adaptive mutations have continued to emerge, causing fresh waves of infection even amongst vaccinated population. The development broad-spectrum antivirals is thus urgently needed. We previously developed two hetero-bivalent nanobodies (Nbs), aRBD-2-5 and aRBD-2-7, potent neutralization activity against the wild-type (WT) Wuhan isolated SARS-CoV-2, by fusing aRBD-2 aRBD-5 aRBD-7, respectively. Here, we resolved crystal structures these Nbs in complex receptor-binding...
Current SARS-CoV-2 Omicron subvariants impose a heavy burden on global health systems by evading immunity from most developed neutralizing antibodies and vaccines. Here, we identified nanobody (aSA3) that strongly cross-reacts with the receptor binding domain (RBD) of both SARS-CoV-1 wild-type (WT) SARS-CoV-2. The dimeric construct aSA3 (aSA3-Fc) tightly binds potently neutralizes WT Based X-ray crystallography, engineered bispecific dimer (2-3-Fc) fusing aSA3-Fc to aRBD-2, previously...
Peanut allergy is a major health problem worldwide. Detection of food allergens critical aspect safety. The VHH domain single chain antibody from camelids, also known as nanobody (Nb), showed its advantages in the development biosensors because high stability, small molecular size, and ease production. However, no specific to peanut has been developed. In this study, we constructed library with random triplets (NNK) CDR regions camel backbone. We screened Ara h 3 obtained several candidate...
Abstract Monitoring the levels of SARS-CoV-2 specific antibodies such as IgG, M and A in COVID-19 patient is an alternative method for diagnosing infection simple way to monitor immune responses convalescent patients after vaccination. Here, we assessed RBD twenty-seven over 28–99 days hospital discharge. Almost all who had severe or moderate symptoms a high-level IgG during hospitalization showed significant reduction at revisit. The remaining low-level stayed low As expected, IgM almost...
Murine caspase-11 is the centerpiece of non-canonical inflammasome pathway that can respond to intracellular LPS and induce pyroptosis. Caspase-11 contains two components, an N-terminal caspase recruitment domain (CARD) a C-terminal catalytic domain. The aggregation thought promote auto-processing activation caspase-11. However, mechanism remains unclear. In this study, we purified CARD fused MBP tag found it tetramerizes in solution. Crystallographic analysis reveals extensive hydrophobic...
Cytosolic inflammasomes are supramolecular complexes that formed in response to intracellular pathogens and danger signals. However, as date, the detailed description of a homotypic caspase recruitment domain (CARD) interaction between NLRP1 ASC has not been presented. We found CARD-CARD purified NLRP1CARD ASCCARD experimentally filamentous complex formation an vitro proteins solution. Moreover, we determined high-resolution crystal structure death fold human ASCCARD. Mutational structural...
A Gram-stain-negative, rod-shaped, exopolysaccharide-producing, strictly aerobic bacterium with a single polar flagellum, designated strain HL22-2(T), was isolated from phosphate mine situated in suburb of Kunmming Yunnan province south-western China. The taxonomic status this evaluated by using polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that HL22-2(T) related to members the genus Pseudomonas. sequence similarities between and Pseudomonas...
The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is major target for antibody therapeutics. Shark-derived variable domains new antigen receptors (VNARs) are smallest fragments with flexible paratopes that can recognize motifs inaccessible to classical antibodies. This study reported four VNARs binders (JM-2, JM-5, JM-17, and JM-18) isolated from Chiloscyllium plagiosum immunized RBD. Biolayer interferometry showed bound RBD an affinity KD ranging 38.5 2720 nM, their Fc...
Abstract The interferon gamma-inducible protein 16 (IFI16) and its murine homologous p204 function in non-sequence specific dsDNA sensing; however, the exact recognition mechanisms of IFI16/p204, which harbour two HIN domains, remain unclear. In present study, we determined crystal structures HINa HINb are highly similar to those other PYHIN family proteins. Moreover, obtained structure HINab domain complex with provided insights into binding mode. binds mainly through α2 helix HINb, linker...
A yellow-pigmented, Gram-stain-negative, strictly aerobic, rod-shaped, round-ended bacterium, designated strain 18-11HK T , was isolated from a phosphate mine situated in the suburb of Kunming Yunnan province south-western China. The taxonomic status this evaluated by using polyphasic approach. On basis 16S rRNA gene sequence similarity, shown to belong genus Novosphingobium showing highest levels similarity with respect ‘ ginsenosidimutans ’ FW-6 (97.2 %), subterraneum DSM 12447 (96.7...