The amnion membrane is developed from embryo-derived cells, and amniotic cells have been shown to exhibit multidifferentiation potential. These represent a desirable source for stem variety of reasons. However, date very few molecular analyses amnion-derived reported, efficient markers isolating the remain unclear. This paper assesses characterization as by examining stemness marker expressions epithelial mesenchymal flow cytometry, immunocytochemistry, quantitative PCR. Flow cytometry...

Human amnion-derived cells are considered to be a promising alternative cell source for their potential clinical use in tissue engineering and regenerative medicine because of proliferation differentiation ability. The can easily obtained from human amnion, offering without medical intervention. It has been proven that express immunosuppressive factors CD59 HLA-G, implying they may have an function. To assess the activity, we investigated effect on NK monocyte Amnion-derived inhibited...

Abstract The development of new three‐dimensional (3D) cell culture system that maintains the physiologically relevant signals hepatocytes is essential in drug discovery and tissue engineering research. Conventional two‐dimensional (2D) yields growth, proliferation, differentiation. However, gene expression signaling profiles can be different from vivo environment. Here, we report fabrication a 3D using an artificial scaffold our custom‐made inkjet bioprinter as strategy for studying...

For hepatic failure, stem cell transplantation has been chosen as an alternative therapy, especially for mesenchymal cells (MSCs). The aim of this study was to investigate the effect eugenol (EUG) on in vivo antifibrotic activity adipose tissue-derived MSCs (AT-MSCs) and underlying mechanism. After characterization MSCs, rats were divided into five groups, Group 1 (normal control), 2 (CCl4), 3 (CCl4 + AT-MSCs), 4 EUG) 5 AT-MSCs EUG). Biochemical histopathological investigations performed....

Background Freshly isolated human amniotic mesenchymal (fHAM) cells contain somatic stem possessing proliferative ability and pluripotency, including a chondrogenic lineage. However, little is known about the biology of amnion-derived (MSCs) because fHAM can barely survive to expand under culture conditions in vitro for long time. Methods In this study, we separated seeded them isolate MSCs analyze its character. addition, suitable growth factor was determined by pellet culture, their...

<b>Objectives</b> Cerebrospinal fluid (CSF) leakage is an undesirable complication of skull base surgery. We used dried human amniotic membrane (AM) as a patch graft for dural repair to determine its efficacy in preventing CSF leakage. <b>Design</b> Frontoparietal craniotomy and removal dura were performed 20 Wistar rats. A AM was placed cover the defect without suturing 16 animals. In four animals, expanded polytetrafluoroethylene implanted. At 2 weeks 1, 3, 6 months, histological...

Severe burn injuries create large skin defects that render the host susceptible to bacterial infections. Burn wound infection often causes systemic sepsis and severe septicemia, resulting in an increase mortality of patients with injuries. Therefore, appropriate care is important prevent improve patient outcomes. However, it difficult heal a third-degree injury. The aim this study was investigate whether hyperdry human amniotic membrane (HD-AM) could promote early granulation tissue...

Mesenchymal stem cells (MSCs) have considerable therapeutic abilities in various disorders, including hepatic fibrosis. They may be affected with different culture conditions. This study investigated, on molecular basics, the effect of pretreatment eugenol characteristics adipose tissue-derived MSCs (ASCs) vitro and implication preconditioning vivo ASCs against CCl4-induced fibrosis rats. The was assessed using viability, scratch migration sphere formation assays. Expressions genes proteins...

Endometrial cancer (EC) is the most common familiar gynecologic malignant tumor identified in female reproductive system and has been increasing yearly. In this study, we will identify surface markers stem cell related with cells (CSCs) of EC. Tissue samples were obtained from endometrial patients during surgical procedures. Single isolated tissues for culturing, transfection into nude mice, histopathology analysis. RT-PCR demonstrated that cultured strongly expressed stemness-related genes,...

Objective: Extracellular matrix (ECM) derived from human amniotic mesenchymal cells (HAMs) has various biological activities. In this study, we developed a novel HAM-derived ECM-coated polylactic-co-glycolic acid (ECM-PLGA) scaffold, examined its property on cells, and investigated potential as cell-free scaffold for cartilage repair. Materials Methods: ECM-PLGA scaffolds were by inoculating HAM PLGA. After decellularization irradiation, accumulated ECM was examined. Exogenous cell growth...

Human amniotic epithelial cells (HAEs) have a low immunogenic profile and possess potent immunosuppressive properties. HAEs also several characteristics similar to stem cells, they are discarded after parturition. Thus, could potentially be used in cell therapy with fewer ethical problems. short life, so our aim is establish characterize immortalized human (iHAEs). were introduced viral oncogenes E6/E7 telomerase reverse transcriptase (hTERT) create iHAEs. These iHAEs proliferated around 200...

Human amniotic membrane (AM) has been used widely as graft biomaterial for a variety of clinical applications. But, there are some persistent problems related to the preparation, storage, and sterilization. To resolve these problems, we developed hyperdry AM (HD-AM) using far-infrared rays, depression air, microwaves then sterilized by γ-ray irradiation. elucidate benefit HD-AM biological materials, compare with physical histological properties freeze-dried (FD-AM) typical methods, evaluate...

Regenerative therapy is a new strategy for the end-stage heart failure; however, ideal cell source has not yet been established this therapy. We expected that amnion might be an cardiac regenerative and differentiation potency of human mesenchymal cells (hAMCs) could improved by overexpression Oct4, key factor maintains undifferentiated state. A plasmid vector was made insertion Oct4 open reading frame (ORF) under control cytomegalovirus (CMV) promoter (pCMV-hOct4) transfected into hAMCs...

Yokoyama, K., Miyatake, S-I., Kajimoto, Y., Kawabata, S., Doi, A., Yoshida, T., Okabe, M., Kirihata, Ono, K. and Kuroiwa, T. Analysis of Boron Distribution In Vivo for Neutron Capture Therapy using Two Different Compounds by Secondary Ion Mass Spectrometry. Radiat. Res. 167, 102–109 (2007).The efficiency boron neutron capture therapy (BNCT) malignant gliomas depends on the selective absolute accumulation 10B atoms in tumor tissues. Only two compounds, BPA BSH, currently can be used...

To overcome the lack of autografts in revision ear surgery, usefulness human amniotic membrane (AM) patches as a dressing substitute for fascia grafts temporal muscle (temporalis graft) was investigated canal wall down tympanoplasty.Retrospective chart review.In 11 ears patients, AM attached over bony surface mastoid cavity (AM group). In nine temporalis graft (fascia The times epithelization were compared both groups.In groups, complete took place all patients. time group 32 days, whereas...

Adult cardiomyocytes have little ability to regenerate, thus cardiac regeneration therapy represents a potential method for treating severe heart failure. Human amniotic mesenchymal cells (hAMCs) the be useful cell source therapy. We attempted isolate stem from hAMCs and differentiate them into cardiomyocytes. Nanog promoter-Cre plasmid cytomegalovirus (CMV) promoter-loxP-STOP-loxP-Red-puro(r) were co-transfected immortalized (iHAMs). Nanog-positive iHAMs treated with 5-azacytidine (5-aza),...