A5068051790- Mycotoxins in Agriculture and Food
- Plant Pathogens and Fungal Diseases
- Plant and fungal interactions
- Plant tissue culture and regeneration
- Pesticide Residue Analysis and Safety
- Wheat and Barley Genetics and Pathology
- Plant Gene Expression Analysis
- Carcinogens and Genotoxicity Assessment
- Phytochemicals and Antioxidant Activities
- Plant biochemistry and biosynthesis
- Plant Toxicity and Pharmacological Properties
- Escherichia coli research studies
- Transgenic Plants and Applications
- Neurological Disease Mechanisms and Treatments
- Microbial infections and disease research
- Genetic and Environmental Crop Studies
- Botanical Research and Chemistry
- Agricultural safety and regulations
- Folate and B Vitamins Research
- Plant nutrient uptake and metabolism
- Ginkgo biloba and Cashew Applications
- Insect and Pesticide Research
- Plant Disease Resistance and Genetics
- Fungal Biology and Applications
- Enzyme Production and Characterization
Sciensano (Belgium)
2019
Veterinary and Agrochemical Research Centre
2002-2018
Instituut voor Landbouw en Visserijonderzoek
1980-1982
A multi-analyte method for the liquid chromatography-tandem mass spectrometric determination of mycotoxins in food supplements is presented. The analytes included and B trichothecenes (nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, neosolaniol, fusarenon-X, diacetoxyscirpenol, HT-2 toxin T-2 toxin), aflatoxins (aflatoxin-B1, aflatoxin-B2, aflatoxin-G1 aflatoxin-G2), Alternaria toxins (alternariol, alternariol methyl ether altenuene), fumonisins (fumonisin-B1,...
Abstract An LC-MS/MS method was developed and validated for the simultaneous determination of deoxynivalenol, zearalenone, T-2-toxin, HT-2-toxin metabolites, including 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, deoxynivalenol-3-glucoside, α-zearalenol, β-zearalenol, zearalenone-4-glucoside, α-zearalenol-4-glucoside, β-zearalenol-4-glucoside zearalenone-4-sulfate in maize, wheat, oats, cornflakes bread. Extraction performed with acetonitrile/water/acetic acid (79/20/1, v/v/v) followed...
The aim of this study was to determine the toxicokinetic characteristics ZEN and its modified forms, α-zearalenol (α-ZEL), β-zearalenol (β-ZEL), zearalenone-14-glucoside (ZEN14G), zearalenone-14-sulfate (ZEN14S), including presystemic systemic hydrolysis in pigs. Crossover pig trials were performed by means intravenous oral administration forms. Systemic plasma concentrations administered toxins their metabolites quantified further processed via tailor-made compartmental models. Furthermore,...
Cross-reactivity of antibodies in AGRAQUANT, DON EIA, VERATOX, ROSA LF-DONQ, and MYCONTROLDON designed for deoxynivalenol (DON) determination food feedstuffs was evaluated against nivalenol, 3-acetylDON, 15-acetylDON, de-epoxy metabolite 1 DON, DON-3β-glucoside, T2-toxin, HT2-toxin, fusarenone X, diacetoxyscirpenol, verrucarol, zearalenone. measurements were run water using the 50% reduction absorbance blank ELISA kits or through direct upon standards mycotoxins via LF-DONQ MYCONTROLDON. For...
In vivo metabolism of masked or conjugated mycotoxins is poorly documented as standards are not commercially available and indirect analysis using hydrolytic enzymes difficult to validate cumbersome. We synthesised zearalenone-14-glucoside (ZEA-14G) chemically. Deoxynivalenol-3-glucuronide (DON-3GlcA) glucuronides 3- 15-acetyl-deoxynivalenol (3- 15-ADON-GlcAs), de-epoxydeoxynivalenol, zearalenone (ZEA), α- β-zearalenol (α- β-ZOL) were rat microsomes. For the first time three ADON-GlcAs...
Pyrrolizidine alkaloids (PAs) are widely distributed plant toxins with species dependent hepatotoxic, carcinogenic, genotoxic and pneumotoxic risks. In a recent European Food Safety Authority (EFSA) opinion, only two data sets from one country were received for honey, while feed set was included. No available food or samples the Belgian market. We developed an LC-MS/MS method, which allowed detection quantification of 16 PAs in broad range matrices sub ng g−1 range. The method validated...
Seven commercially available deoxynivalenol (DON) and zearalenone (ZEN) immunoaffinity columns (IACs) were tested for cross-reactivity to conjugated forms (3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, DON-3-glucoside, DON-3-glucuronide, ZEN-glucosides, ZEN-glucuronide) metabolites (de-epoxydeoxynivalenol, α-zearalenol, β-zearalenol) nivalenol (NIV), using a semi-quantitative multi-mycotoxin ultra-performance liquid chromatography-tandem mass spectrometry method. The DON IACs showed...
Mycotoxins are harmful food contaminants. Currently, human exposure assessment to these toxins is often based on calculations combining mycotoxin occurrence data in with population consumption. Because of limitations inherent that approach, biomarkers have been proposed as a suitable alternative whereby more accurate at the individual level can be performed. The BIOMYCO study designed assess using urinary exposure. Over different seasons 2013 and 2014, morning urine gathered representative...
Over a 4-year period (2010–13), survey aiming at determining the occurrence of Fusarium spp. and their relations to mycotoxins in mature grains took place southern Belgium. The most prevalent species were F. graminearum, avenaceum, poae culmorum, with large variations between years locations. An even proportion mating type found for cerealis tricinctum is usually sign ongoing sexual recombination. In contrast, an unbalanced was no MAT1-2 allele present langsethiae population. Genetic...
Ochratoxin A (OTA), one of the most toxic mycotoxins, can contaminate a wide range food and feedstuff. To date, data on its conjugates via glucuronidation request clarification consolidation. In present study, combined approaches ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), UHPLC-Orbitrap-high resolution (HRMS) chromatography-multiple stage (LC-MSn) were utilized to investigate metabolic profile OTA in rat liver microsomes. Three conjugated products...
Enniatins, a large group of cyclodepsipeptides, are widely distributed contaminants different crops intended for human and animal consumption. Enniatin B is one the principal analogues in species genus Fusarium , known to have ionophoric, antibiotic, insecticidal activity. Regardless considerable cytotoxic effects observed vitro enniatins been characterised as compounds with low acute toxicity vivo. The biotransformation enniatin has previously elucidated liver microsomes, 12 metabolites (M1...
Mycotoxins may cause deleterious effects (among others nephrogenic, hepatogenic, carcinogenic, teratogenic, neurogenic) in animals and humans, therefore they have been intensely studied monitored over the years. For citrinin (CIT), a nephrotoxic mycotoxin, however, this has not yet case. According to latest European Food Safety Authority report, correct risk assessment of CIT was possible due lack occurrence data. Besides, traces or its metabolite, dehydrocitrinone are widely (in up 90%...