A5005509086- Environmental Toxicology and Ecotoxicology
- Immune Cell Function and Interaction
- Aquaculture Nutrition and Growth
- Parvovirus B19 Infection Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Aquaculture disease management and microbiota
- Enzyme function and inhibition
- Dietary Effects on Health
- Metabolomics and Mass Spectrometry Studies
- Reproductive biology and impacts on aquatic species
- SARS-CoV-2 and COVID-19 Research
- Escherichia coli research studies
- Toxin Mechanisms and Immunotoxins
- Sepsis Diagnosis and Treatment
- Adipose Tissue and Metabolism
- Respiratory viral infections research
- Biochemical Analysis and Sensing Techniques
- Synthesis and Catalytic Reactions
- Pharmaceutical and Antibiotic Environmental Impacts
- Advanced Chemical Sensor Technologies
- Clostridium difficile and Clostridium perfringens research
- Olfactory and Sensory Function Studies
- Genomics and Phylogenetic Studies
- Asthma and respiratory diseases
- Mercury impact and mitigation studies
Washington University in St. Louis
2021-2024
Sidra Medical and Research Center
2021
AXYS Technologies (Canada)
2016
University of Victoria
2016
University of Ottawa
2016
Ontario Genomics
2016
University of California, Davis
1994-2014
Fisheries and Oceans Canada
2007-2010
University of British Columbia
2007-2010
Life-threatening pulmonary influenza can be caused by inborn errors of type I and III IFN immunity. We report a 5-yr-old child with severe at 2 yr. She is homozygous for loss-of-function IRF9 allele. Her cells activate gamma-activated factor (GAF) STAT1 homodimers but not IFN-stimulated gene 3 (ISGF3) trimers (STAT1/STAT2/IRF9) in response to IFN-α2b. The transcriptome induced IFN-α2b the patient's much narrower than that control cells; however, induction subset transcripts remains...
Four endemic human coronaviruses (HCoVs) are commonly associated with acute respiratory infection in humans. B cell responses to these "common cold" viruses remain incompletely understood. Here we report a comprehensive analysis of CoV-specific antibody repertoires 231 children and 1168 adults using phage immunoprecipitation sequencing. Seroprevalence antibodies against HCoVs ranged between approximately 4% 27% depending on the species cohort. We identified at least 136 novel linear...
CstII from bacterium Campylobacter jejuni strain OH4384 has been previously characterized as a bifunctional sialyltransferase having both alpha2,3-sialyltransferase (GM3 oligosaccharide synthase) and alpha2,8-sialyltransferase (GD3 activities which catalyze the transfer of N-acetylneuraminic acid (Neu5Ac) cytidine 5'-monophosphate (CMP)-Neu5Ac to C-3' galactose in lactose C-8 Neu5Ac 3'-sialyllactose, respectively (Gilbert M, Karwaski MF, Bernatchez S, Young NM, Taboada E, Michniewicz J,...
Sensitive and quantitative protocols for characterizing low-dose effects are needed to meet the demands of 21st century chemical hazard assessment. To test hypothesis that xenobiotic exposure at environmentally relevant concentrations produces specific biochemical fingerprints in organisms, metabolomic perturbations zebrafish (Danio rerio) embryo/larvae were measured following 24 h exposures 13 individual chemicals covering a wide range contaminant classes. Measured metabolites (208 total)...
The sense of smell helps us navigate the environment, but its molecular architecture and underlying logic remain understudied. spatial location odorant receptor genes (Olfrs) in nose is thought to be independent structural diversity odorants they detect. Using transcriptomics, we create a genome-wide 3D atlas mouse olfactory mucosa (OM). Topographic maps differentially expressed space reveal that both Olfrs non-Olfrs are distributed continuous overlapping fashion over at least five broad...
Trans-sialidases catalyze the transfer of a sialic acid from one sialoside to an acceptor form new sialoside. α2,3-Trans-sialidase activity was initially discovered in parasitic protozoan Trypanosoma cruzi, and more recently found multifunctional Pasteurella multocida sialyltransferase PmST1. α2,8-Trans-sialidase also described for Campylobacter jejuni CstII. We report here discovery α2,6-trans-sialidase previously reported recombinant truncated bacterial α2,6-sialyltransferase...
Abstract Four endemic human coronaviruses (HCoVs) are commonly associated with acute respiratory infection in humans. B cell responses to these “common cold” viruses remain incompletely understood. Here we report a comprehensive analysis of CoV-specific antibody repertoires 231 children and 1168 adults using phage-immunoprecipitation sequencing. Seroprevalence antibodies HCoVs ranged between ~4 27% depending on the species cohort. We identified at least 136 novel linear epitopes. Antibody...
Abstract Sepsis is an aberrant systemic inflammatory response mediated by the acute activation of innate immune system. Neutrophils are important contributors to that controls infection, but harbour risk collateral tissue damage such as thrombosis and organ dysfunction. A better understanding modulations cellular processes in neutrophils other blood cells during sepsis needed can be initiated via transcriptomic profile investigations. To point, growing repertoire publicly accessible datasets...
In addition to its canonical functions, vitamin D has been proposed be an important mediator of the immune system. Despite ample sunshine, deficiency is prevalent (>80%) in Middle East, resulting a high rate supplementation. However, underlying molecular mechanisms specific regimen prescribed and potential factors affecting individual's response supplementation are not well characterized. Our objective describe changes blood transcriptome explore associated with D3 one hundred D-deficient...
Background: We have previously shown coxsackievirus B (CVB) to be a potent inducer of congenital heart disease (CHD) in mice. The clinical relevance these findings humans and the roles other viruses pathogenesis CHD remain unknown. Methods: obtained plasma samples, collected at all trimesters, from 89 subjects (104 pregnancies), 73 healthy controls (88 16 with CHD–affected birth (16 Perinatal Family Tissue Bank (PFTB). performed CVB IgG/IgM serological assays on plasma. also used ViroCap...
Fasting regimens can promote health, mitigate chronic immunological disorders, and improve age-related pathophysiological parameters in animals humans. Several ongoing clinical trials are using fasting as a potential therapy for various conditions. alters metabolism by acting reset energy homeostasis, but the molecular mechanisms underlying beneficial effects of short-term (STF) not well understood, particularly at systems or multiorgan level. We performed RNA-sequencing nine organs from...
Abstract Background and aims The task of identifying a representative yet manageable target gene list for assessing the pathogenesis complicated multifaceted diseases is challenging. Using Inflammatory Bowel Disease (IBD) as an example, we conceived bioinformatic approach to identify novel genes associated with various disease subtypes, in combination known clinical control genes. Methods From available literature, used Acumenta Literature Lab TM (LitLab), network analyses, LitLab Gene...
ABSTRACT The sense of smell helps us navigate the environment, but its molecular architecture and underlying logic remain unknown. spatial location odorant receptor genes ( Olfrs ) in nose is widely thought to be independent structural diversity odorants they detect. Using transcriptomics, we created a genome-wide 3D atlas mouse olfactory mucosa (OM). Topographic maps differentially expressed space reveal that both non- are distributed continuous overlapping fashion over five broad zones OM....
Modulation of individual macronutrients or caloric density is known to regulate host resistance infection in mice. However, the impact diet composition, independent macronutrient and energy content, on susceptibility unclear. We show that two laboratory rodent diets, widely used as standard animal feeds experimental controls, display distinct abilities supporting mice during influenza infection. Mice placed highly processed AIN93G showed increased mortality compared with those a grain-based...
Sepsis results from the dysregulation of host immune system. This highly variable disease affects 19 million people globally, and accounts for 5 deaths annually. In transcriptomic datasets curated public repositories, we observed a consistent upregulation (3.26–5.29 fold) ERLIN1—a gene coding an ER membrane prohibitin regulator inositol 1, 4, 5-trisphosphate receptors sterol regulatory element-binding proteins—under septic conditions in healthy neutrophils, monocytes, whole blood. vitro...
Abstract Prevalence of allergies has reached ~20% population in developed countries and sensitization rate to one or more allergens among school age children are approaching 50%. However, the combination complexity atopic allergy susceptibility/development environmental factors made identification gene biomarkers challenging. The amount publicly accessible transcriptomic data presents an unprecedented opportunity for mechanistic discoveries validation complex disease signatures across...