A5013804525- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Head and Neck Cancer Studies
- Long-Term Effects of COVID-19
- Vestibular and auditory disorders
- Olfactory and Sensory Function Studies
- Anatomy and Medical Technology
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Lung Cancer Research Studies
- Immune Cell Function and Interaction
- Surgical Simulation and Training
- Retinal and Optic Conditions
- Hearing Loss and Rehabilitation
- Cancer Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Peptidase Inhibition and Analysis
- Chemotherapy-induced organ toxicity mitigation
- Protease and Inhibitor Mechanisms
- Oral and Maxillofacial Pathology
- Healthcare Operations and Scheduling Optimization
- COVID-19 and healthcare impacts
- Cardiac, Anesthesia and Surgical Outcomes
- Allergic Rhinitis and Sensitization
Universitätsklinik für Hals-, Nasen- und Ohrenheilkunde
2019-2024
Heidelberg University
2020-2024
University Medical Centre Mannheim
2020-2024
University Hospital Heidelberg
2020-2024
Medizinische Fakultät Mannheim
2021-2024
Universitäts HNO Klinik Mannheim
2021
UPMC Hillman Cancer Center
2017-2020
University of Duisburg-Essen
2017-2019
Essen University Hospital
2018-2019
University of Pittsburgh
2017-2019
Abstract Purpose: Head and neck cancers (HNCs) often induce profound immunosuppression, which contributes to disease progression interferes with immune-based therapies. Body fluids of patients HNC are enriched in exosomes potentially engaged negative regulation antitumor immune responses. The presence content derived from plasma evaluated for the ability dysfunction influence activity. Experimental Design: Exosomes were isolated by size-exclusion chromatography 38 14 healthy donors....
ABSTRACT Tumour‐derived exosomes (TEX) are a subset of extracellular vesicles (EVs) present in body fluids patients with cancer. The role this exosome melanoma progression has been interest ever since ex vivo studies produced by cell lines were shown to suppress anti‐melanoma immune responses. To study the impact melanoma‐derived (MTEX) patients’ plasma on progression, isolation MTEX from total is necessary. We have developed an immunoaffinity‐based method for capture patients. Using mAb...
A method for isolation of exosomes from tumor cell supernatants or cancer patients' plasma is presented. Tumor-derived (TEX) are defined as a subset extracellular vesicles (EVs) sized at 30 to 150 nm and originating multivesicular bodies (MVBs). The utilizes size exclusion chromatography (SEC) recovery cell-line plasma. recovered morphologically intact, aggregate-free, functionally competent. Their molecular content parallels that the parent cells they carry various immunoregulatory ligands...
Exosomes produced by tumor cells have been shown to reprogram functions of human immune cells. Molecular cargos exosomes isolated from supernatants HPV(+) and HPV(-) head neck cancer (HNC) cell lines or HNC patients' plasma were compared. The exosome protein profiles resembled those respective parent Only carried E6/E7, p16 survivin. negative for cyclin D1 low p53 levels. Immunomodulatory molecules (TGF-β, FasL, OX40, OX40L HSP70) exosomes. These co-incubated with T induced apoptosis...
Background: Various symptoms have been associated with COVID-19, but little is known about the impacts of COVID-19 on sensory system, risk factors, and duration symptoms. This study assesses olfactory, gustatory, hearing, vestibular systems after COVID-19. Methods: cross-sectional, single-center involved 50 patients one to six months reports their patient records extent, onset, balance disorders using questionnaires during Sensory were objectively studied following clinical tests Sniffin’...
<b><i>Introduction:</i></b> A 2½ D point cloud registration method was developed to generate digital twins of different tissue shapes and resection cavities by applying a machine learning (ML) approach. This demonstrates the feasibility quantifying soft shifts. <b><i>Methods:</i></b> An ML model trained using simulated surface scan data obtained from tumor resections in pig head cadaver model. It hereby uses 438 scans surface. Tissue shift...
Neutrophils are known to play an important regulatory role during tumor progression in several types of cancer. However, the mechanisms responsible for their tumorigenic bias and extended lifespan cancer not clear date. This study uncovers a previously unknown mechanism by which tumor-derived small extracellular vesicles (sEVs), via serpin cargo, reprogram neutrophils adopt tumor-supporting phenotype. We demonstrated here elevated content plasma sEVs head neck progression, significant cargo...
Human papillomavirus (HPV) is an etiologic factor in head and neck squamous cell carcinoma (HNSCC). HPV(+) cancers respond favorably to therapy potentially due more robust anti-tumor immune responses. We hypothesized that tumor-derived exosomes (TEX) produced by or HPV(-) HNSCCs differentially modulate responses.Proteomes of from HNSCC lines were compared search for proteins putatively involved the communication with system. TEX isolated supernatants (SCC-2, SCC-47, SCC-90) (PCI-13 PCI-30)...
Plasma-derived exosomes of head and neck squamous cell carcinoma (HNSCC) patients carry inhibitory factors mediating immune suppression. Separation tumor-derived (TEX) non-TEX may assist in a better understanding their respective parental cells. Here, we evaluate the impact TEX or hematopoietic-derived on We evaluated apoptosis CD8+ T cells, conversion CD4+ cells to regulatory (Treg), adenosine production by TEX, non-TEX, total exosomes. Exosome protein cargo was significantly higher CD45(−)...
Abstract Small extracellular vesicles from saliva (SEVs) have high potential as biomarkers in Head and Neck cancer (HNC). However, there is no common consensus on the ideal method for their isolation. This study compared different ultracentrifugation (UC) methods (durations + /− additional purification) with size exclusion chromatography (SEC) investigated of SEVs diagnostic biological activity NK CD8 T cells. 19 HNC patients 8 healthy donors (HDs) were thoroughly characterized. Transmission...
During the COVID-19 pandemic, adverse neurological effects have been described. In addition to unspecific symptoms, cranial nerve deficits appeared as part of SARS-CoV-2 infection. this case report, we describe a 74-year-old patient who developed bilateral paralysis vocal cords some weeks following his dismissal in stable condition after pneumonia. After ruling out central lesions, peripheral tumors, and other possible causes, therapy was initiated with methylprednisolone, inhalations,...
Background: Head and neck squamous cell carcinomas (HNSCC) lack tumor-specific biomarkers. Exosomes from HNSCC patients carry immunomodulatory molecules, correlate with clinical parameters. We compared miRNA profiles of plasma- saliva-derived exosomes to reveal liquid biomarker candidates for HNSCC. Methods: were isolated by differential ultracentrifugation corresponding plasma saliva samples 11 five healthy donors (HD). Exosomal profiles, as determined nCounter® SPRINT technology, analyzed...
Introduction Checkpoint inhibitors, such as PD1 represent an important pillar in the therapy of advanced malignancies head and neck region. The most relevant complications are immune-related adverse effects (irAEs), which immense burden for patients. Currently, no sufficient stratification measures available to identify patients at increased risk irAEs. aim this retrospective study was examine whether demographic, histopathological, clinical, or laboratory values start CPI a factor later...
Head and neck squamous cell carcinomas (HNSCC) are highly immune suppressive aggressive malignancies. As part of the tumor microenvironment, exosomes contribute to this suppression. The Fc receptor CD16 is widely expressed on monocytes, neutrophils, natural killer (NK) cells involved in antibody-dependent cell-mediated cytotoxicity (ADCC). Here, surface levels total tumor-derived (TEX) from plasma HNSCC patients were analyzed regarding their potential as liquid biomarkers for disease stage....
Human papillomavirus (HPV)(+) and HPV(−) head neck cancer (HNC) cells’ interactions with the host immune system are poorly understood. Recently, we identified molecular functional differences in exosomes produced by HPV(+) vs. cells, suggesting that genetic cargos of might identify novel biomarkers HPV-related HNCs. Exosomes were isolated size exclusion chromatography from supernatants three two HNC cell lines. Paired lysates analyzed for messenger RNA (mRNA) qRT-PCR microRNA (miR) contents...
Head and Neck Cancers (HNCs) have highly immunosuppressive properties. Small extracellular vesicles (sEVs), including exosomes, nanosized mediators of intercellular communication in the blood, carry proteins effectively inhibit anti-tumor immune responses HNCs. This study evaluates markers on sEVs from 40 HNC patients at different disease stages 3- 6-month follow-up after surgery and/or chemoradiotherapy. As controls, normal donors (NDs) are examined. Immunoregulatory surface were detected...
Abstract Background In Head and neck cancer (HNC) angiogenesis is essential for tumor progression metastasis. Small extracellular vesicles (sEVs) from HNC cell lines alter endothelial (EC) functions towards a pro-angiogenic phenotype. However, the role of plasma sEVs retrieved patients in this process not clear so far. Methods Plasma were isolated on size exclusion chromatography columns 32 (early-stage UICC I/II: 8, advanced-stage III/IV: 24), 12 with no evident disease after therapy (NED)...
Although checkpoint inhibitors (CPI) have recently extended the treatment options and improved clinical response of advanced stage head neck squamous cell carcinoma (HNSCC), success remains unpredictable. Programmed death ligand‑1 (PD‑L1) is a key player in immunotherapy. Tumor cells, exosomes derived therefrom, are carriers PD‑L1 efficiently suppress immune responses. The aim present study was to analyze influence established therapies on expression HNSCC lines their exosomes. lines,...
: Hereditary Hemorrhagic Telangiectasia (HHT) is a genetic disorder leading to frequent bleeding in several organs. As HHT diagnosis demanding and depends on clinical criteria, liquid biopsy would be beneficial. Exosomes from biofluids are nano-sized vesicles for intercellular communication. Their cargo characteristics represent biomarkers many diseases. Here, exosomes of patients were examined regarding their biosignature.
The symptoms and severity of SARS-CoV-2 infection vary greatly across the spectrum, from asymptomatic to severe pneumonia with acute respiratory distress syndrome even death. Dizziness is a frequently reported symptom viral infection. However, extent which this results effect on vestibular system remains unclear.In present single-center, prospective cohort study, patients previous underwent assessment consisting Handicap Inventory assess dizziness during after infection, clinical...